Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line

Schizophyllum commune is a filamentous fungus widely known and distributed across the world continents. Although S. commune was acknowledged for its medicinal properties in countries like China and Japan, the Malaysian strain of S. Commune has not been fully utilized and developed. These basidiom...

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Main Authors:	Abd rahman, Nur Rahilah, Ahmad Raus, Raha, Mel, Maizirwan
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	TP248.13 Biotechnology TP500 Fermentation industries. Beverages. Alcohol
Online Access:	http://irep.iium.edu.my/27360/ http://irep.iium.edu.my/27360/1/FULL_PAPER_iccbpe.pdf

id	iium-27360
recordtype	eprints
spelling	iium-273602012-12-14T06:17:15Z http://irep.iium.edu.my/27360/ Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line Abd rahman, Nur Rahilah Ahmad Raus, Raha Mel, Maizirwan TP248.13 Biotechnology TP500 Fermentation industries. Beverages. Alcohol Schizophyllum commune is a filamentous fungus widely known and distributed across the world continents. Although S. commune was acknowledged for its medicinal properties in countries like China and Japan, the Malaysian strain of S. Commune has not been fully utilized and developed. These basidiomycetes can be found abundantly in rubber plantations across Malaysia especially after raining season. S. commune excreted an exopolysaccharide known as Schizophyllan which can be identified as a homoGlucan containing a β-(1,3)-linked backbone with single β-(1,6)-linked glucose side chains at every third residue. Schizophyllan is believed to have an antiviral, antimicrobial as well as anticancer activity. The purpose of this study is to observe the cytotoxicity effect of S. commune extract towards the DF-1 cell line. The selected Malaysian strain of S.commune mycelia were cultivated by liquid fermentation using shake flasks in selective media and under certain condition to produce the secondary bioactive compound. Compound was extracted by addition of isopropanol that will be separated later using centrifugation. The analysis of S. commune extract by FTIR showed the weak bond at 890 cm-1 as evidenced of the β-glycosidic linkage characteristic. The cytotoxicity analysis showed only 37 percent of inhibition rate of DF-1 cell line viability for 1 mg/ml of S. commune extract and the value of IC50 is higher 100 mg/ml showed there was no cytotoxic effect. Therefore, the S. commune is safe to be used for further study involving this cell line. 2012-11-21 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/27360/1/FULL_PAPER_iccbpe.pdf Abd rahman, Nur Rahilah and Ahmad Raus, Raha and Mel, Maizirwan (2012) Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line. In: 26th Symposium of Malaysian Chemicals Engineers in conjunction with the 4th International Conference on Chemical and Bioprocess Engineering, 21-23 November 2012, Kota Kinabalu.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology TP500 Fermentation industries. Beverages. Alcohol
spellingShingle	TP248.13 Biotechnology TP500 Fermentation industries. Beverages. Alcohol Abd rahman, Nur Rahilah Ahmad Raus, Raha Mel, Maizirwan Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line
description	Schizophyllum commune is a filamentous fungus widely known and distributed across the world continents. Although S. commune was acknowledged for its medicinal properties in countries like China and Japan, the Malaysian strain of S. Commune has not been fully utilized and developed. These basidiomycetes can be found abundantly in rubber plantations across Malaysia especially after raining season. S. commune excreted an exopolysaccharide known as Schizophyllan which can be identified as a homoGlucan containing a β-(1,3)-linked backbone with single β-(1,6)-linked glucose side chains at every third residue. Schizophyllan is believed to have an antiviral, antimicrobial as well as anticancer activity. The purpose of this study is to observe the cytotoxicity effect of S. commune extract towards the DF-1 cell line. The selected Malaysian strain of S.commune mycelia were cultivated by liquid fermentation using shake flasks in selective media and under certain condition to produce the secondary bioactive compound. Compound was extracted by addition of isopropanol that will be separated later using centrifugation. The analysis of S. commune extract by FTIR showed the weak bond at 890 cm-1 as evidenced of the β-glycosidic linkage characteristic. The cytotoxicity analysis showed only 37 percent of inhibition rate of DF-1 cell line viability for 1 mg/ml of S. commune extract and the value of IC50 is higher 100 mg/ml showed there was no cytotoxic effect. Therefore, the S. commune is safe to be used for further study involving this cell line.
format	Conference or Workshop Item
author	Abd rahman, Nur Rahilah Ahmad Raus, Raha Mel, Maizirwan
author_facet	Abd rahman, Nur Rahilah Ahmad Raus, Raha Mel, Maizirwan
author_sort	Abd rahman, Nur Rahilah
title	Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line
title_short	Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line
title_full	Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line
title_fullStr	Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line
title_full_unstemmed	Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line
title_sort	cytotoxicity study of schizophyllum commune extract towards df-1 cell line
publishDate	2012
url	http://irep.iium.edu.my/27360/ http://irep.iium.edu.my/27360/1/FULL_PAPER_iccbpe.pdf
first_indexed	2023-09-18T20:40:39Z
last_indexed	2023-09-18T20:40:39Z
_version_	1777409342833688576

Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line

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