$In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd$

In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd

Medicinal use of Acrostichum aureum and A. speciosum in wound healing treatment by Malay community was tested scientifically in this project. Wound healing properties of ethanolic, aqueous extracts and the fractions of A. aureum and A. speciosum were evaluated in vitro using scratch wound assay on N...

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Main Authors:	Herman, Hendy Putra, Darnis, Deny Susanti, Saad, Shahbudin, Bakhtiar, M. Taher, Ramli, Norazsida
Format:	Conference or Workshop Item
Language:	English
Published:	Ibnu Sina Institute, UTM 2012
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/27426/ http://irep.iium.edu.my/27426/ http://irep.iium.edu.my/27426/1/ISPC_2012_PROCEEDING_BOOK.pdf

id	iium-27426
recordtype	eprints
spelling	iium-274262017-06-15T06:12:20Z http://irep.iium.edu.my/27426/ In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd Herman, Hendy Putra Darnis, Deny Susanti Saad, Shahbudin Bakhtiar, M. Taher Ramli, Norazsida RS Pharmacy and materia medica Medicinal use of Acrostichum aureum and A. speciosum in wound healing treatment by Malay community was tested scientifically in this project. Wound healing properties of ethanolic, aqueous extracts and the fractions of A. aureum and A. speciosum were evaluated in vitro using scratch wound assay on NIH/3T3 cell line. In total, 22 different extracts and fractions from leaves, stems, and rhizomes of both species were used for this testing. NIH/ 3T3 cell lines were seeded at a density of 5 x 104 cells/ mL in 24-well plate and incubated at 37°C until reaching nearly confluence. The confluent cell monolayer was manually scratched with a sterile pipette tip. This test was done in triplicate. For the treatment, each well was treated with 0.2 mg/ mL of the different extracts, 2 ng/ mL of platelet derived growth factor (PDGF) as positive control and DMEM medium as negative control. The images before treatment and after 12 hour post-treatment were photographed using ProgRes® CapturePro 2.5 software to measure mean migration rate (μm/hour) and open image area (%) using TScratch software. The results showed that cell line treated with aqueous extract of rhizomes and stems of both species have improved the migration rate. The fractions of A. aureum (n-Hexane, n- Hexane: DCM, and ethanol) and fractions of A. speciosum (n-Hexane, n- Hexane: DCM, and DCM) have shown enhancing effect in wound healing. These results strongly suggested the beneficial effects of both plant extracts for wound healing treatment and thus justified their medicinal use. Ibnu Sina Institute, UTM 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/27426/1/ISPC_2012_PROCEEDING_BOOK.pdf Herman, Hendy Putra and Darnis, Deny Susanti and Saad, Shahbudin and Bakhtiar, M. Taher and Ramli, Norazsida (2012) In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd. In: International Science Postgraduate Conference 2012 (ISPC 2012), 27-30 November 2012, Skudai, Johor. http://www.fs.utm.my/ispc2012/index.php/welcoming-message-nspc
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Herman, Hendy Putra Darnis, Deny Susanti Saad, Shahbudin Bakhtiar, M. Taher Ramli, Norazsida In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd
description	Medicinal use of Acrostichum aureum and A. speciosum in wound healing treatment by Malay community was tested scientifically in this project. Wound healing properties of ethanolic, aqueous extracts and the fractions of A. aureum and A. speciosum were evaluated in vitro using scratch wound assay on NIH/3T3 cell line. In total, 22 different extracts and fractions from leaves, stems, and rhizomes of both species were used for this testing. NIH/ 3T3 cell lines were seeded at a density of 5 x 104 cells/ mL in 24-well plate and incubated at 37°C until reaching nearly confluence. The confluent cell monolayer was manually scratched with a sterile pipette tip. This test was done in triplicate. For the treatment, each well was treated with 0.2 mg/ mL of the different extracts, 2 ng/ mL of platelet derived growth factor (PDGF) as positive control and DMEM medium as negative control. The images before treatment and after 12 hour post-treatment were photographed using ProgRes® CapturePro 2.5 software to measure mean migration rate (μm/hour) and open image area (%) using TScratch software. The results showed that cell line treated with aqueous extract of rhizomes and stems of both species have improved the migration rate. The fractions of A. aureum (n-Hexane, n- Hexane: DCM, and ethanol) and fractions of A. speciosum (n-Hexane, n- Hexane: DCM, and DCM) have shown enhancing effect in wound healing. These results strongly suggested the beneficial effects of both plant extracts for wound healing treatment and thus justified their medicinal use.
format	Conference or Workshop Item
author	Herman, Hendy Putra Darnis, Deny Susanti Saad, Shahbudin Bakhtiar, M. Taher Ramli, Norazsida
author_facet	Herman, Hendy Putra Darnis, Deny Susanti Saad, Shahbudin Bakhtiar, M. Taher Ramli, Norazsida
author_sort	Herman, Hendy Putra
title	In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd
title_short	In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd
title_full	In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd
title_fullStr	In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd
title_full_unstemmed	In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd
title_sort	in vitro evaluation of wound healing properties of fractions and extracts of acrostichum aureum linné and a. speciosum willd
publisher	Ibnu Sina Institute, UTM
publishDate	2012
url	http://irep.iium.edu.my/27426/ http://irep.iium.edu.my/27426/ http://irep.iium.edu.my/27426/1/ISPC_2012_PROCEEDING_BOOK.pdf
first_indexed	2023-09-18T20:40:45Z
last_indexed	2023-09-18T20:40:45Z
_version_	1777409348788551680

In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum Aureum Linné and A. Speciosum Willd

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