Bromelain production: Current trends and perspective

Bromelain is a mixture of proteolytic enzymes derived from the stems and juice of pineapples. It has been extensively used in food industry; for meat tenderization, baking processes, and in prevention of browning of apple juice. Stem bromelain is a highly accepted phytotherapeutic agent. It has an...

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Main Authors:	Bala, Muntari, Ismail, Nurul Azira, Mel, Maizirwan, Jami, Mohammed Saedi, Salleh, Hamzah Mohd., Amid, Azura
Format:	Article
Language:	English
Published:	Museum Hist Naturelle 2012
Subjects:	Q Science (General) T Technology (General)
Online Access:	http://irep.iium.edu.my/28364/ http://irep.iium.edu.my/28364/1/Bromelain_review.pdf

id	iium-28364
recordtype	eprints
spelling	iium-283642013-04-24T01:13:52Z http://irep.iium.edu.my/28364/ Bromelain production: Current trends and perspective Bala, Muntari Ismail, Nurul Azira Mel, Maizirwan Jami, Mohammed Saedi Salleh, Hamzah Mohd. Amid, Azura Q Science (General) T Technology (General) Bromelain is a mixture of proteolytic enzymes derived from the stems and juice of pineapples. It has been extensively used in food industry; for meat tenderization, baking processes, and in prevention of browning of apple juice. Stem bromelain is a highly accepted phytotherapeutic agent. It has anti-tumor and antiinflammatory effects, use in wound healing, as digestive aids, etc. This review aims at presenting a detailed account on the level of achievement on bromelain production from the conventional methods. Specifically, the isolation, purification and the biochemical properties of bromelain are discussed. The study also focused on the current trends and the progress made in the production of recombinant stem bromelain in Escherichia coli. This involved cloning of stem bromelain gene and its expression, optimization of labscale fermentation conditions for E. coli harboring recombinant bromelain as well as the enzyme thermal and storage stability measured using differential scanning calorimetry. It is hoped that this review would provide relevant information to contemporary researchers who are active in the field of bromelain and other related proteases. Museum Hist Naturelle 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/28364/1/Bromelain_review.pdf Bala, Muntari and Ismail, Nurul Azira and Mel, Maizirwan and Jami, Mohammed Saedi and Salleh, Hamzah Mohd. and Amid, Azura (2012) Bromelain production: Current trends and perspective. Archives Des Sciences, 65 (11). pp. 369-399. ISSN 1661-464X
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) T Technology (General)
spellingShingle	Q Science (General) T Technology (General) Bala, Muntari Ismail, Nurul Azira Mel, Maizirwan Jami, Mohammed Saedi Salleh, Hamzah Mohd. Amid, Azura Bromelain production: Current trends and perspective
description	Bromelain is a mixture of proteolytic enzymes derived from the stems and juice of pineapples. It has been extensively used in food industry; for meat tenderization, baking processes, and in prevention of browning of apple juice. Stem bromelain is a highly accepted phytotherapeutic agent. It has anti-tumor and antiinflammatory effects, use in wound healing, as digestive aids, etc. This review aims at presenting a detailed account on the level of achievement on bromelain production from the conventional methods. Specifically, the isolation, purification and the biochemical properties of bromelain are discussed. The study also focused on the current trends and the progress made in the production of recombinant stem bromelain in Escherichia coli. This involved cloning of stem bromelain gene and its expression, optimization of labscale fermentation conditions for E. coli harboring recombinant bromelain as well as the enzyme thermal and storage stability measured using differential scanning calorimetry. It is hoped that this review would provide relevant information to contemporary researchers who are active in the field of bromelain and other related proteases.
format	Article
author	Bala, Muntari Ismail, Nurul Azira Mel, Maizirwan Jami, Mohammed Saedi Salleh, Hamzah Mohd. Amid, Azura
author_facet	Bala, Muntari Ismail, Nurul Azira Mel, Maizirwan Jami, Mohammed Saedi Salleh, Hamzah Mohd. Amid, Azura
author_sort	Bala, Muntari
title	Bromelain production: Current trends and perspective
title_short	Bromelain production: Current trends and perspective
title_full	Bromelain production: Current trends and perspective
title_fullStr	Bromelain production: Current trends and perspective
title_full_unstemmed	Bromelain production: Current trends and perspective
title_sort	bromelain production: current trends and perspective
publisher	Museum Hist Naturelle
publishDate	2012
url	http://irep.iium.edu.my/28364/ http://irep.iium.edu.my/28364/1/Bromelain_review.pdf
first_indexed	2023-09-18T20:41:51Z
last_indexed	2023-09-18T20:41:51Z
_version_	1777409418125639680

Bromelain production: Current trends and perspective

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