Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients

Frequent loss of heterozygosity (LOH) and mutations of the tumor suppressor gene PTEN (phosphatase and tensin homologue deleted from chromosome 10) have been found in sporadic gliomas. The most documented regions of allelic losses include 9p21, 10q23-25 and 17p13 whereas PTEN aberrations are prefere...

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Main Authors:	Zainuddin, Norafiza, Jaafar, Hasnan, Isa, Mohd. Nizam, Abdullah, Jafri Malin
Format:	Article
Language:	English
Published:	Faculty of Tropical Medicine, Mahidol University 2005
Subjects:	R Medicine (General) RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/28467/ http://irep.iium.edu.my/28467/1/J_Trop_Med.pdf

id	iium-28467
recordtype	eprints
spelling	iium-284672013-09-14T05:37:20Z http://irep.iium.edu.my/28467/ Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin R Medicine (General) RC0254 Neoplasms. Tumors. Oncology (including Cancer) Frequent loss of heterozygosity (LOH) and mutations of the tumor suppressor gene PTEN (phosphatase and tensin homologue deleted from chromosome 10) have been found in sporadic gliomas. The most documented regions of allelic losses include 9p21, 10q23-25 and 17p13 whereas PTEN aberrations are preferentially found in glioblastoma multiformes. This research aimed to detect the incidence of allelic losses on chromosomes 10q, 9p, 17p and 13q and mutations on exons 5, 6 and 8 of PTEN in malignant gliomas. Malignant glioma specimens obtained were classified histopathologically according to the WHO criteria. Each tumor was then subjected to polymerase chain reaction (PCR)-LOH analysis using microsatellite markers and single-stranded conformational polymorphism (SSCP) analysis. Twelve of 23 (52%) malignant glioma cases showed allelic losses whereas 7 of 23 (30%) samples showed aberrant band patterns and mutations of PTEN. Four of these cases showed LOH in 10q23 and mutations of PTEN. The data on LOH indicated the involvement of different genes in the genesis of glioma whereas mutations of PTEN indicated the role of PTEN tumor suppressor gene in the progression of glioma in Malay population. Faculty of Tropical Medicine, Mahidol University 2005-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/28467/1/J_Trop_Med.pdf Zainuddin, Norafiza and Jaafar, Hasnan and Isa, Mohd. Nizam and Abdullah, Jafri Malin (2005) Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients. Southeast Asian Journal of Tropical Medicine and Public Health, 36 (3). pp. 748-756. ISSN 0125-1562
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General) RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	R Medicine (General) RC0254 Neoplasms. Tumors. Oncology (including Cancer) Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients
description	Frequent loss of heterozygosity (LOH) and mutations of the tumor suppressor gene PTEN (phosphatase and tensin homologue deleted from chromosome 10) have been found in sporadic gliomas. The most documented regions of allelic losses include 9p21, 10q23-25 and 17p13 whereas PTEN aberrations are preferentially found in glioblastoma multiformes. This research aimed to detect the incidence of allelic losses on chromosomes 10q, 9p, 17p and 13q and mutations on exons 5, 6 and 8 of PTEN in malignant gliomas. Malignant glioma specimens obtained were classified histopathologically according to the WHO criteria. Each tumor was then subjected to polymerase chain reaction (PCR)-LOH analysis using microsatellite markers and single-stranded conformational polymorphism (SSCP) analysis. Twelve of 23 (52%) malignant glioma cases showed allelic losses whereas 7 of 23 (30%) samples showed aberrant band patterns and mutations of PTEN. Four of these cases showed LOH in 10q23 and mutations of PTEN. The data on LOH indicated the involvement of different genes in the genesis of glioma whereas mutations of PTEN indicated the role of PTEN tumor suppressor gene in the progression of glioma in Malay population.
format	Article
author	Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin
author_facet	Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin
author_sort	Zainuddin, Norafiza
title	Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients
title_short	Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients
title_full	Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients
title_fullStr	Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients
title_full_unstemmed	Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients
title_sort	malignant glioma: the involvement of loss of allelic heterozygosity and pten mutations in a group of malay patients
publisher	Faculty of Tropical Medicine, Mahidol University
publishDate	2005
url	http://irep.iium.edu.my/28467/ http://irep.iium.edu.my/28467/1/J_Trop_Med.pdf
first_indexed	2023-09-18T20:41:58Z
last_indexed	2023-09-18T20:41:58Z
_version_	1777409425549557760

Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients

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