Neuroregenerative property of haruan (Channa striatus spp.) traditional extract

Among key neurorestorative strategy in treating neurodegenerative diseases and brain trauma is neurostimulation using non-endogenous substances, thus improving regeneration and repair potential of neurons. The purpose of this work is to treat PC12 cells, an established neuronal differentiation model...

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Main Authors:	Mohd Shafri, Mohd. Affendi, Mat Jais, Abdul Manan, Kyu, Kim Min
Format:	Article
Language:	English
Published:	UiTM Perlis 2011
Subjects:	QP Physiology R Medicine (General) RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/28701/ http://irep.iium.edu.my/28701/ http://irep.iium.edu.my/28701/1/ma_mohd_shafri.pdf

id	iium-28701
recordtype	eprints
spelling	iium-287012013-02-13T08:13:54Z http://irep.iium.edu.my/28701/ Neuroregenerative property of haruan (Channa striatus spp.) traditional extract Mohd Shafri, Mohd. Affendi Mat Jais, Abdul Manan Kyu, Kim Min QP Physiology R Medicine (General) RS Pharmacy and materia medica Among key neurorestorative strategy in treating neurodegenerative diseases and brain trauma is neurostimulation using non-endogenous substances, thus improving regeneration and repair potential of neurons. The purpose of this work is to treat PC12 cells, an established neuronal differentiation model, with a traditional extract of haruan, Channa striatus spp, an indigenous riverine fish of Southeast Asian countries, and analyse PC12 cells’ morphologic and behavioural changes at different concentrations of the extract. PC12 cells were first cultured at 1x103 cells/cm2 density in 12 well plate in an incubator (5% CO2, 37°C), each containing 1ml of complete growth medium (Eagle’s Minimum Essential Medium with 10% foetal bovine serum), for 48 hours, to reach a 70% confluency. Media was removed and changed, and treated with 100μl, 200μl, 300μl and 400μl of haruan traditional extract (HTE). PC12 cells were observed under camera-equipped inverted microscope at 24, 48, 72 and 96 hours. Neurite outgrowth as well as growth behaviour were noted in comparison to negative (1ml complete growth medium) and positive (1ml cyclic AMP) controls. 100μl of HTE was demonstrated as the optimum concentration at which multiaxonal and multipolar cells and longest neurite outgrowth were seen. The result is suggesting that haruan traditional extract (HTE) is able to exert desirable effects in morphology and behaviour of PC12 cells and may potentially be useful therapeutically in the genesis and repair of neuron cells. UiTM Perlis 2011-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/28701/1/ma_mohd_shafri.pdf Mohd Shafri, Mohd. Affendi and Mat Jais, Abdul Manan and Kyu, Kim Min (2011) Neuroregenerative property of haruan (Channa striatus spp.) traditional extract. Jurnal Intelek, 6 (1). pp. 77-83. ISSN 2231-7716 http://jurnalintelek.uitm.edu.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QP Physiology R Medicine (General) RS Pharmacy and materia medica
spellingShingle	QP Physiology R Medicine (General) RS Pharmacy and materia medica Mohd Shafri, Mohd. Affendi Mat Jais, Abdul Manan Kyu, Kim Min Neuroregenerative property of haruan (Channa striatus spp.) traditional extract
description	Among key neurorestorative strategy in treating neurodegenerative diseases and brain trauma is neurostimulation using non-endogenous substances, thus improving regeneration and repair potential of neurons. The purpose of this work is to treat PC12 cells, an established neuronal differentiation model, with a traditional extract of haruan, Channa striatus spp, an indigenous riverine fish of Southeast Asian countries, and analyse PC12 cells’ morphologic and behavioural changes at different concentrations of the extract. PC12 cells were first cultured at 1x103 cells/cm2 density in 12 well plate in an incubator (5% CO2, 37°C), each containing 1ml of complete growth medium (Eagle’s Minimum Essential Medium with 10% foetal bovine serum), for 48 hours, to reach a 70% confluency. Media was removed and changed, and treated with 100μl, 200μl, 300μl and 400μl of haruan traditional extract (HTE). PC12 cells were observed under camera-equipped inverted microscope at 24, 48, 72 and 96 hours. Neurite outgrowth as well as growth behaviour were noted in comparison to negative (1ml complete growth medium) and positive (1ml cyclic AMP) controls. 100μl of HTE was demonstrated as the optimum concentration at which multiaxonal and multipolar cells and longest neurite outgrowth were seen. The result is suggesting that haruan traditional extract (HTE) is able to exert desirable effects in morphology and behaviour of PC12 cells and may potentially be useful therapeutically in the genesis and repair of neuron cells.
format	Article
author	Mohd Shafri, Mohd. Affendi Mat Jais, Abdul Manan Kyu, Kim Min
author_facet	Mohd Shafri, Mohd. Affendi Mat Jais, Abdul Manan Kyu, Kim Min
author_sort	Mohd Shafri, Mohd. Affendi
title	Neuroregenerative property of haruan (Channa striatus spp.) traditional extract
title_short	Neuroregenerative property of haruan (Channa striatus spp.) traditional extract
title_full	Neuroregenerative property of haruan (Channa striatus spp.) traditional extract
title_fullStr	Neuroregenerative property of haruan (Channa striatus spp.) traditional extract
title_full_unstemmed	Neuroregenerative property of haruan (Channa striatus spp.) traditional extract
title_sort	neuroregenerative property of haruan (channa striatus spp.) traditional extract
publisher	UiTM Perlis
publishDate	2011
url	http://irep.iium.edu.my/28701/ http://irep.iium.edu.my/28701/ http://irep.iium.edu.my/28701/1/ma_mohd_shafri.pdf
first_indexed	2023-09-18T20:42:16Z
last_indexed	2023-09-18T20:42:16Z
_version_	1777409443590307840

Neuroregenerative property of haruan (Channa striatus spp.) traditional extract

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