Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells

The antioxidant behaviour of major dietary carotenoids accumulated at high concentrations in human hepatoma HepG2 cells was evaluated, in comparison with a-tocopherol. The cells that accumulated carotenoids and a-tocopherol at levels higher than the values reported in the human liver were exposed...

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Main Authors:	Jaswir, Irwandi, Kobayashi, Miyuki, Koyama, Toshie, Kotake-Nara, Eiichi, Nagao, Akihiko
Format:	Article
Language:	English
Published:	Elsevier 2012
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/28710/ http://irep.iium.edu.my/28710/ http://irep.iium.edu.my/28710/ http://irep.iium.edu.my/28710/1/Foodchem_%282012%29-AO_behaviour.pdf

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spelling	iium-287102013-03-20T01:37:20Z http://irep.iium.edu.my/28710/ Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells Jaswir, Irwandi Kobayashi, Miyuki Koyama, Toshie Kotake-Nara, Eiichi Nagao, Akihiko QD Chemistry The antioxidant behaviour of major dietary carotenoids accumulated at high concentrations in human hepatoma HepG2 cells was evaluated, in comparison with a-tocopherol. The cells that accumulated carotenoids and a-tocopherol at levels higher than the values reported in the human liver were exposed to mild oxidative stress with tert-butylhydroperoxide. b-Carotene (>2.6 nmol/mg protein) and astaxanthin (>1.8 nmol/mg protein) significantly suppressed lipid peroxidation, while b-cryptoxanthin and lutein did not. a-Tocopherol remarkably suppressed lipid peroxidation with an IC50 value of 0.16 nmol/mg protein. Neither a-tocopherol nor any of the carotenoids except for lycopene showed pro-oxidant action even at high cellular concentrations. The antioxidant behaviours of carotenoids in a cellular milieu were quite different from those previously found in liposomes and homogeneous solutions. Further studies are required to assess the implications of the antioxidant behaviours found in the cultured cells on human health. Elsevier 2012-05-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/28710/1/Foodchem_%282012%29-AO_behaviour.pdf Jaswir, Irwandi and Kobayashi, Miyuki and Koyama, Toshie and Kotake-Nara, Eiichi and Nagao, Akihiko (2012) Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells. Food Chemistry, 132 (2). pp. 865-872. ISSN 0308-8146 http://www.sciencedirect.com/science/article/pii/S0308814611016359# 10.1016/j.foodchem.2011.11.053
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Jaswir, Irwandi Kobayashi, Miyuki Koyama, Toshie Kotake-Nara, Eiichi Nagao, Akihiko Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells
description	The antioxidant behaviour of major dietary carotenoids accumulated at high concentrations in human hepatoma HepG2 cells was evaluated, in comparison with a-tocopherol. The cells that accumulated carotenoids and a-tocopherol at levels higher than the values reported in the human liver were exposed to mild oxidative stress with tert-butylhydroperoxide. b-Carotene (>2.6 nmol/mg protein) and astaxanthin (>1.8 nmol/mg protein) significantly suppressed lipid peroxidation, while b-cryptoxanthin and lutein did not. a-Tocopherol remarkably suppressed lipid peroxidation with an IC50 value of 0.16 nmol/mg protein. Neither a-tocopherol nor any of the carotenoids except for lycopene showed pro-oxidant action even at high cellular concentrations. The antioxidant behaviours of carotenoids in a cellular milieu were quite different from those previously found in liposomes and homogeneous solutions. Further studies are required to assess the implications of the antioxidant behaviours found in the cultured cells on human health.
format	Article
author	Jaswir, Irwandi Kobayashi, Miyuki Koyama, Toshie Kotake-Nara, Eiichi Nagao, Akihiko
author_facet	Jaswir, Irwandi Kobayashi, Miyuki Koyama, Toshie Kotake-Nara, Eiichi Nagao, Akihiko
author_sort	Jaswir, Irwandi
title	Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells
title_short	Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells
title_full	Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells
title_fullStr	Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells
title_full_unstemmed	Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells
title_sort	antioxidant behaviour of carotenoids highly accumulated in hepg2 cells
publisher	Elsevier
publishDate	2012
url	http://irep.iium.edu.my/28710/ http://irep.iium.edu.my/28710/ http://irep.iium.edu.my/28710/ http://irep.iium.edu.my/28710/1/Foodchem_%282012%29-AO_behaviour.pdf
first_indexed	2023-09-18T20:42:16Z
last_indexed	2023-09-18T20:42:16Z
_version_	1777409444346331136

Antioxidant behaviour of carotenoids highly accumulated in HepG2 cells

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