Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes

The effect of the recently developed graphene nanoflakes (GNFs) on the polymerase chain reaction (PCR) has been investigated in this paper. The rationale behind the use of GNFs is their unique physical and thermal properties. Experiments show that GNFs can enhance the thermal conductivity of base fl...

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Main Authors:	Khaliq R., Abdul, Kafafy, Raed, Salleh, Hamzah Mohd., Faris, Waleed Fekry
Format:	Article
Language:	English
Published:	Institute of Physics Publishing 2012
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/28976/ http://irep.iium.edu.my/28976/ http://irep.iium.edu.my/28976/ http://irep.iium.edu.my/28976/1/28976.pdf

id	iium-28976
recordtype	eprints
spelling	iium-289762013-08-16T07:02:12Z http://irep.iium.edu.my/28976/ Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes Khaliq R., Abdul Kafafy, Raed Salleh, Hamzah Mohd. Faris, Waleed Fekry T Technology (General) The effect of the recently developed graphene nanoflakes (GNFs) on the polymerase chain reaction (PCR) has been investigated in this paper. The rationale behind the use of GNFs is their unique physical and thermal properties. Experiments show that GNFs can enhance the thermal conductivity of base fluids and results also revealed that GNFs are a potential enhancer of PCR efficiency; moreover, the PCR enhancements are strongly dependent on GNF concentration. It was found that GNFs yield DNA product equivalent to positive control with up to 65% reduction in the PCR cycles. It was also observed that the PCR yield is dependent on the GNF size, wherein the surface area increases and augments thermal conductivity. Computational fluid dynamics (CFD) simulations were performed to analyze the heat transfer through the PCR tube model in the presence and absence of GNFs. The results suggest that the superior thermal conductivity effect of GNFs may be the main cause of the PCR enhancement. Institute of Physics Publishing 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/28976/1/28976.pdf Khaliq R., Abdul and Kafafy, Raed and Salleh, Hamzah Mohd. and Faris, Waleed Fekry (2012) Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes. Nanotechnology, 23 (45). pp. 1-8. ISSN 0957-4484 http://www.scopus.com/record/display.url?eid=2-s2.0-84867835798&origin=resultslist&sort=plf-f&src=s&st1=Enhancing+the+efficiency+of+polymerase+chain+reaction+using+graphene+nanoflakes&sid=BE4280E0FF992461630A0426C5E8ED79.aXczxbyuHHiXgaIW6Ho7g%3a20&sot=b&s 10.1088/0957-4484/23/45/455106
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Khaliq R., Abdul Kafafy, Raed Salleh, Hamzah Mohd. Faris, Waleed Fekry Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes
description	The effect of the recently developed graphene nanoflakes (GNFs) on the polymerase chain reaction (PCR) has been investigated in this paper. The rationale behind the use of GNFs is their unique physical and thermal properties. Experiments show that GNFs can enhance the thermal conductivity of base fluids and results also revealed that GNFs are a potential enhancer of PCR efficiency; moreover, the PCR enhancements are strongly dependent on GNF concentration. It was found that GNFs yield DNA product equivalent to positive control with up to 65% reduction in the PCR cycles. It was also observed that the PCR yield is dependent on the GNF size, wherein the surface area increases and augments thermal conductivity. Computational fluid dynamics (CFD) simulations were performed to analyze the heat transfer through the PCR tube model in the presence and absence of GNFs. The results suggest that the superior thermal conductivity effect of GNFs may be the main cause of the PCR enhancement.
format	Article
author	Khaliq R., Abdul Kafafy, Raed Salleh, Hamzah Mohd. Faris, Waleed Fekry
author_facet	Khaliq R., Abdul Kafafy, Raed Salleh, Hamzah Mohd. Faris, Waleed Fekry
author_sort	Khaliq R., Abdul
title	Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes
title_short	Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes
title_full	Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes
title_fullStr	Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes
title_full_unstemmed	Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes
title_sort	enhancing the efficiency of polymerase chain reaction using graphene nanoflakes
publisher	Institute of Physics Publishing
publishDate	2012
url	http://irep.iium.edu.my/28976/ http://irep.iium.edu.my/28976/ http://irep.iium.edu.my/28976/ http://irep.iium.edu.my/28976/1/28976.pdf
first_indexed	2023-09-18T20:42:33Z
last_indexed	2023-09-18T20:42:33Z
_version_	1777409461843918848

Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes

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