Extraction and evaluation of antibacterial activity from selected flowering plants
The huge diversity of Malaysian flora has various chemical constituents that make them as outstanding natural product candidates for the treatment of infectious diseases. The screening practice for phytochemical compound in them is essential to explore more natural sources to replace synthetic an...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Science Publications
2012
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/29228/ http://irep.iium.edu.my/29228/ http://irep.iium.edu.my/29228/ http://irep.iium.edu.my/29228/1/Abdullah_et_al_American_Medical_J.pdf |
| Summary: | The huge diversity of Malaysian flora has various chemical constituents that make them as
outstanding natural product candidates for the treatment of infectious diseases. The screening practice
for phytochemical compound in them is essential to explore more natural sources to replace synthetic
antibiotics, which generally have side effects such as hypersensitivity, immune-suppression and
allergic reactions. Antibacterial activities have been detected in some of the Malaysian plants and most
of the Malaysian medicinal plants have been screened for this property. However, so far no study has
focus on Malaysian flowering plants yet. In this study, the extraction and determination of antibacterial
property from 19 Malaysian flowering plants were conducted. The plants were extracted with
methanol, ethyl acetate, hexane and distilled water, individually at concentration of 0.1g mL-1. The
extraction process condition was set to 300 rpm agitation for 10 h at room temperature. The crude
extracts of each plant (5 mg/disc) were tested against Bacillus subtilis and Escherichia coli using
agar disc diffusion assay method. The screening results showed that ethyl acetate extract of
Spathiphyllum cannifolium (‘peace lily’) leaves possesses the highest antibacterial activity against B.
subtilis with zone of inhibition of 25 mm. Most of the plant samples extracted with methanol and
ethyl acetate have indicated positive activity toward B. subtilis growth. However, the hexane and
distilled water extracts was ineffective to combat the B. subtilis growth. Unfortunately, all of the
extracts were not active against E. coli. This study suggested that S. cannifolium is highly potential
in antibacterial activity which can be further analyzed for the development of new antibiotic
exclusively for gram positive bacteria. |
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