Site directed mutagenesis to improve phyfauia1 phytase activity for animal feed
Phytate is largely unavailable to monogastric animal such as swine, poultry and fish, as they lack of sufficient endogenous enzymatic activity to hydrolyze phytate. The result is the elimination of precious nutrients that would be beneficial to their growth; furthermore, they will excrete most of th...
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2011
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| Online Access: | http://irep.iium.edu.my/29245/ http://irep.iium.edu.my/29245/1/hamzah_site_directed.pdf |
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iium-292452013-02-27T09:25:22Z http://irep.iium.edu.my/29245/ Site directed mutagenesis to improve phyfauia1 phytase activity for animal feed Mohd. Salleh, Hamzah Samsuddin , Nurhusna TP248.13 Biotechnology TP368 Food processing and manufacture Phytate is largely unavailable to monogastric animal such as swine, poultry and fish, as they lack of sufficient endogenous enzymatic activity to hydrolyze phytate. The result is the elimination of precious nutrients that would be beneficial to their growth; furthermore, they will excrete most of the indigestive phytate which can contribute to phosphorus being over applied to the land. Phosphorus has a beneficial impact on vegetative growth on land as well as marine vegetation, causing an increased growth o weeds. This enhanced vegetation consumes large amounts of oxygen, resulting in the loss of aquatic life and ultimately contributes to water pollution and eutrophication of ground water and aquatic environment. Phytase, a type of histidine acid phosphatase hydrolyzes phytin phosphorus and when present in an animal's digestive tract, benefits the animal while reducing total phosphorus levels in manure. Computer modelling is used to identify and examine the active site of phytase. The factors influencing the ligand binding strength in the active site is analyzed and computational site directed mutagenesis experiments were carried out to evaluate the effects of mutations on the binding strength before and after mutation. From the directive results of computational studies, point mutation was introduced by site directed mutagenesis using polymerase chain reaction (PCR). The activity was measured by kinetic characterization with phytate as a substrate. Decrease in KM notable in all functional mutants indicates that all mutant shows increment in substrate binding. Two functional mutant shows improvement in activity of phytase. 2011 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/29245/1/hamzah_site_directed.pdf Mohd. Salleh, Hamzah and Samsuddin , Nurhusna (2011) Site directed mutagenesis to improve phyfauia1 phytase activity for animal feed. In: International Conference on Biotechnology Engineering, ICBioE’11, 17-19 May, 2011 , Kuala Lumpur. |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
TP248.13 Biotechnology TP368 Food processing and manufacture |
| spellingShingle |
TP248.13 Biotechnology TP368 Food processing and manufacture Mohd. Salleh, Hamzah Samsuddin , Nurhusna Site directed mutagenesis to improve phyfauia1 phytase activity for animal feed |
| description |
Phytate is largely unavailable to monogastric animal such as swine, poultry and fish, as they lack of sufficient endogenous enzymatic activity to hydrolyze phytate. The result is the elimination of precious nutrients that would be beneficial to their growth; furthermore, they will excrete most of the indigestive phytate which can contribute to phosphorus being over applied to the land. Phosphorus has a beneficial impact on vegetative growth on land as well as marine vegetation, causing an increased growth o weeds. This enhanced vegetation consumes large amounts of oxygen, resulting in the loss of aquatic life and ultimately contributes to water pollution and eutrophication of ground water and aquatic environment. Phytase, a type of histidine acid phosphatase hydrolyzes phytin phosphorus and when present in an animal's digestive tract, benefits the animal while reducing total phosphorus levels in manure. Computer modelling is used to identify and examine the active site of phytase. The factors influencing the ligand binding strength in the active site is analyzed and computational site directed mutagenesis experiments were carried out to evaluate the effects of mutations on the binding strength before and after mutation. From the directive results of computational studies, point mutation was introduced by site directed mutagenesis using polymerase chain reaction (PCR). The activity was measured by kinetic characterization with phytate as a substrate. Decrease in KM notable in all functional mutants indicates that all mutant shows increment in substrate binding. Two functional mutant shows improvement in activity of phytase. |
| format |
Conference or Workshop Item |
| author |
Mohd. Salleh, Hamzah Samsuddin , Nurhusna |
| author_facet |
Mohd. Salleh, Hamzah Samsuddin , Nurhusna |
| author_sort |
Mohd. Salleh, Hamzah |
| title |
Site directed mutagenesis to improve phyfauia1 phytase activity for animal feed
|
| title_short |
Site directed mutagenesis to improve phyfauia1 phytase activity for animal feed
|
| title_full |
Site directed mutagenesis to improve phyfauia1 phytase activity for animal feed
|
| title_fullStr |
Site directed mutagenesis to improve phyfauia1 phytase activity for animal feed
|
| title_full_unstemmed |
Site directed mutagenesis to improve phyfauia1 phytase activity for animal feed
|
| title_sort |
site directed mutagenesis to improve phyfauia1 phytase activity for animal feed |
| publishDate |
2011 |
| url |
http://irep.iium.edu.my/29245/ http://irep.iium.edu.my/29245/1/hamzah_site_directed.pdf |
| first_indexed |
2023-09-18T20:42:55Z |
| last_indexed |
2023-09-18T20:42:55Z |
| _version_ |
1777409484410322944 |