Comparison of growth rate and viability of DF-1 cell line in different culture media

One of the commercial cell line that gained attention for the study of viral vaccine was an immortalized cell line derived from chicken embryo fibroblast (CEF) cells. Unlike CEF cells, DF-1 cells have higher proliferation rate and also can be subcultured infinite number of times. In this study, th...

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Main Authors: Arifin, Mohd Azmir, Mel, Maizirwan, Ahmad Raus, Raha, S. H., Sharifah, Ideris, Aini
Format: Conference or Workshop Item
Language:English
Published: 2008
Subjects:
Online Access:http://irep.iium.edu.my/29284/
http://irep.iium.edu.my/29284/1/zPaper_no_14_for_ICSTIE__Mohd_Azmir-edited_.pdf
id iium-29284
recordtype eprints
spelling iium-292842013-04-24T08:10:05Z http://irep.iium.edu.my/29284/ Comparison of growth rate and viability of DF-1 cell line in different culture media Arifin, Mohd Azmir Mel, Maizirwan Ahmad Raus, Raha S. H., Sharifah Ideris, Aini Q Science (General) SF Animal culture One of the commercial cell line that gained attention for the study of viral vaccine was an immortalized cell line derived from chicken embryo fibroblast (CEF) cells. Unlike CEF cells, DF-1 cells have higher proliferation rate and also can be subcultured infinite number of times. In this study, the growth profile of DF-1 cell line was investigated by undergoing series of optimization process by testing it out on several culture media and also by manipulating the components of culture media which were sodium bicarbonate and serum. At the end of this study it has been figured out that DF-1 achieved the highest cell culture density in Dulbecco’s Modification of Eagle’s Medium( DMEM) and with 1.24 g/L of NaHCO3 and 5.5 % serum, it yielded the shortest doubling time. 2008 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/29284/1/zPaper_no_14_for_ICSTIE__Mohd_Azmir-edited_.pdf Arifin, Mohd Azmir and Mel, Maizirwan and Ahmad Raus, Raha and S. H., Sharifah and Ideris, Aini (2008) Comparison of growth rate and viability of DF-1 cell line in different culture media. In: 2nd International Conference on Science and Technology. , 12 - 13 December 2008, Pulau Pinang.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic Q Science (General)
SF Animal culture
spellingShingle Q Science (General)
SF Animal culture
Arifin, Mohd Azmir
Mel, Maizirwan
Ahmad Raus, Raha
S. H., Sharifah
Ideris, Aini
Comparison of growth rate and viability of DF-1 cell line in different culture media
description One of the commercial cell line that gained attention for the study of viral vaccine was an immortalized cell line derived from chicken embryo fibroblast (CEF) cells. Unlike CEF cells, DF-1 cells have higher proliferation rate and also can be subcultured infinite number of times. In this study, the growth profile of DF-1 cell line was investigated by undergoing series of optimization process by testing it out on several culture media and also by manipulating the components of culture media which were sodium bicarbonate and serum. At the end of this study it has been figured out that DF-1 achieved the highest cell culture density in Dulbecco’s Modification of Eagle’s Medium( DMEM) and with 1.24 g/L of NaHCO3 and 5.5 % serum, it yielded the shortest doubling time.
format Conference or Workshop Item
author Arifin, Mohd Azmir
Mel, Maizirwan
Ahmad Raus, Raha
S. H., Sharifah
Ideris, Aini
author_facet Arifin, Mohd Azmir
Mel, Maizirwan
Ahmad Raus, Raha
S. H., Sharifah
Ideris, Aini
author_sort Arifin, Mohd Azmir
title Comparison of growth rate and viability of DF-1 cell line in different culture media
title_short Comparison of growth rate and viability of DF-1 cell line in different culture media
title_full Comparison of growth rate and viability of DF-1 cell line in different culture media
title_fullStr Comparison of growth rate and viability of DF-1 cell line in different culture media
title_full_unstemmed Comparison of growth rate and viability of DF-1 cell line in different culture media
title_sort comparison of growth rate and viability of df-1 cell line in different culture media
publishDate 2008
url http://irep.iium.edu.my/29284/
http://irep.iium.edu.my/29284/1/zPaper_no_14_for_ICSTIE__Mohd_Azmir-edited_.pdf
first_indexed 2023-09-18T20:42:58Z
last_indexed 2023-09-18T20:42:58Z
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