Growth study of DF-1 cell line in microcarrier bioreactor

Animal cell culture is fundamental in the manufacturing of many biological products. Biological products produced in animal cell cultures include vaccines, enzymes, hormones, antibodies and anticancer agents. However majority of animal cells is anchorage-dependent and requires attachment to a surf...

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Main Authors:	Arifin, Mohd Azmir, Mel, Maizirwan, Ahmad Raus, Raha, S. H., Sharifah, Ideris, Aini
Format:	Conference or Workshop Item
Language:	English
Published:	2008
Subjects:	Q Science (General) SF Animal culture
Online Access:	http://irep.iium.edu.my/29285/ http://irep.iium.edu.my/29285/1/zPaper_no_31_for_ICSTIE__Maizirwan-edited_.pdf

id	iium-29285
recordtype	eprints
spelling	iium-292852013-04-24T08:05:51Z http://irep.iium.edu.my/29285/ Growth study of DF-1 cell line in microcarrier bioreactor Arifin, Mohd Azmir Mel, Maizirwan Ahmad Raus, Raha S. H., Sharifah Ideris, Aini Q Science (General) SF Animal culture Animal cell culture is fundamental in the manufacturing of many biological products. Biological products produced in animal cell cultures include vaccines, enzymes, hormones, antibodies and anticancer agents. However majority of animal cells is anchorage-dependent and requires attachment to a surface for their growth. Microcarrier is a small bead used to provide surface for the culture of anchorage-dependent animal cells. Microcarrier culture has very large surface area to volume ratio, thus able to produce larger number of cells when compared to conventional monolayer cultures which uses multiple glass or plastic bottles. This research was carried out mainly to study the growth performance of DF-1 cell culture by using microcarriers in a 1-L litre stirred tank bioreactor and compares it with conventional monolayer cultures grown in T-flasks bioreactor. At the end of the study it has been found that the maximum viable cell concentration achieved by the microcarrier culture was 1.315 × 106 cells/ml which was higher than maximum viable cell concentration achieved by the cell culture in T-flasks which was 1.25 × 106 cells/ml. 2008 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/29285/1/zPaper_no_31_for_ICSTIE__Maizirwan-edited_.pdf Arifin, Mohd Azmir and Mel, Maizirwan and Ahmad Raus, Raha and S. H., Sharifah and Ideris, Aini (2008) Growth study of DF-1 cell line in microcarrier bioreactor. In: 2nd International Conference on Science and Technology., 12 - 13 December 2008, Pulau Pinang.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) SF Animal culture
spellingShingle	Q Science (General) SF Animal culture Arifin, Mohd Azmir Mel, Maizirwan Ahmad Raus, Raha S. H., Sharifah Ideris, Aini Growth study of DF-1 cell line in microcarrier bioreactor
description	Animal cell culture is fundamental in the manufacturing of many biological products. Biological products produced in animal cell cultures include vaccines, enzymes, hormones, antibodies and anticancer agents. However majority of animal cells is anchorage-dependent and requires attachment to a surface for their growth. Microcarrier is a small bead used to provide surface for the culture of anchorage-dependent animal cells. Microcarrier culture has very large surface area to volume ratio, thus able to produce larger number of cells when compared to conventional monolayer cultures which uses multiple glass or plastic bottles. This research was carried out mainly to study the growth performance of DF-1 cell culture by using microcarriers in a 1-L litre stirred tank bioreactor and compares it with conventional monolayer cultures grown in T-flasks bioreactor. At the end of the study it has been found that the maximum viable cell concentration achieved by the microcarrier culture was 1.315 × 106 cells/ml which was higher than maximum viable cell concentration achieved by the cell culture in T-flasks which was 1.25 × 106 cells/ml.
format	Conference or Workshop Item
author	Arifin, Mohd Azmir Mel, Maizirwan Ahmad Raus, Raha S. H., Sharifah Ideris, Aini
author_facet	Arifin, Mohd Azmir Mel, Maizirwan Ahmad Raus, Raha S. H., Sharifah Ideris, Aini
author_sort	Arifin, Mohd Azmir
title	Growth study of DF-1 cell line in microcarrier bioreactor
title_short	Growth study of DF-1 cell line in microcarrier bioreactor
title_full	Growth study of DF-1 cell line in microcarrier bioreactor
title_fullStr	Growth study of DF-1 cell line in microcarrier bioreactor
title_full_unstemmed	Growth study of DF-1 cell line in microcarrier bioreactor
title_sort	growth study of df-1 cell line in microcarrier bioreactor
publishDate	2008
url	http://irep.iium.edu.my/29285/ http://irep.iium.edu.my/29285/1/zPaper_no_31_for_ICSTIE__Maizirwan-edited_.pdf
first_indexed	2023-09-18T20:42:58Z
last_indexed	2023-09-18T20:42:58Z
_version_	1777409487977578496

Growth study of DF-1 cell line in microcarrier bioreactor

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