Assessment of ibuprofen, non-selective cox inhibitor, as a neuroprotective agent in Alzheimer’s model of rats

Aging related reduction in cerebral blood flow (CBF) has been associated with neurodegenerative disorders including Alzheimer’s disease and dementia. Experimentally, a condition of chronic cerebral hypoperfusion due to reduced CBF can be induced by permanent bilateral occlusion of common carotid art...

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Main Authors:	Saxena, Anil Kumar, Mohd, Fadly, Talib, Norlelawati A.
Format:	Conference or Workshop Item
Language:	English English
Published:	2013
Subjects:	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/29564/ http://irep.iium.edu.my/29564/ http://irep.iium.edu.my/29564/1/IRIIE_POSTER_1.pdf http://irep.iium.edu.my/29564/4/Assessment_of_ibuprofen%2C_non-selective_cox_inhibitor%2C_as_a_neuroprotective_agent_in_Alzheimer%E2%80%99s_model_of_rats.pdf

id	iium-29564
recordtype	eprints
spelling	iium-295642013-08-15T05:27:26Z http://irep.iium.edu.my/29564/ Assessment of ibuprofen, non-selective cox inhibitor, as a neuroprotective agent in Alzheimer’s model of rats Saxena, Anil Kumar Mohd, Fadly Talib, Norlelawati A. RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action RS Pharmacy and materia medica Aging related reduction in cerebral blood flow (CBF) has been associated with neurodegenerative disorders including Alzheimer’s disease and dementia. Experimentally, a condition of chronic cerebral hypoperfusion due to reduced CBF can be induced by permanent bilateral occlusion of common carotid arteries (2-vessel occlusion, 2VO) in rats. Since neuroinflammation, leading to neuronal apoptosis and death, is one of the mechanisms which is thought to play a significant role in chronic degenerative neurological disorders, the present study was planned to assess the neuroprotective role of ibuprofen in chronic cerebral hypoperfusion-induced neurodegeneration. After acclimatization, fifteen Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A – sham control, Group B – 2VO, and Group C – 2VO-I (treated daily with ibuprofen, 50 mg/kg, orally following 2VO). On 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal CA-1 region, hippocampal COX-2 mRNA expression and prostaglandin E2 (PGE-2) levels were estimated. There was a significant difference in neuronal cell death, increase in COX-2 mRNA expression and PGE-2 levels in 2VO group as compared to sham control group. In ibuprofen-treated 2VO (2VO-I) rats, the viable neuronal cell count of the hippocampal CA-1 region was significantly higher as compared to the untreated 2VO group. For the hippocampal COX-2 mRNA expression, the value in the ibuprofen treated group showed insignificant difference when compared to the untreated 2VO group. However, hippocampal PGE-2 levels were found to be significantly lower in the ibuprofen treated 2VO rats as compared to untreated 2VO rats. These results clearly indicate that ibuprofen is an effective neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats and can be fruitfully utilized in the management of Alzheimer’s disease. 2013-02-19 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/29564/1/IRIIE_POSTER_1.pdf application/pdf en http://irep.iium.edu.my/29564/4/Assessment_of_ibuprofen%2C_non-selective_cox_inhibitor%2C_as_a_neuroprotective_agent_in_Alzheimer%E2%80%99s_model_of_rats.pdf Saxena, Anil Kumar and Mohd, Fadly and Talib, Norlelawati A. (2013) Assessment of ibuprofen, non-selective cox inhibitor, as a neuroprotective agent in Alzheimer’s model of rats. In: IIUM Research, Invention and Innovation Exhibition (IRIIE) 2013, 19-20 Feb 2013, Cultural Activity Centre (CAC) and KAED Gallery, IIUM. http://www.iium.edu.my/irie/13/index.php/8-iriie/1-iium-research-invention-and-innovation-exhibition-2013
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action RS Pharmacy and materia medica
spellingShingle	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action RS Pharmacy and materia medica Saxena, Anil Kumar Mohd, Fadly Talib, Norlelawati A. Assessment of ibuprofen, non-selective cox inhibitor, as a neuroprotective agent in Alzheimer’s model of rats
description	Aging related reduction in cerebral blood flow (CBF) has been associated with neurodegenerative disorders including Alzheimer’s disease and dementia. Experimentally, a condition of chronic cerebral hypoperfusion due to reduced CBF can be induced by permanent bilateral occlusion of common carotid arteries (2-vessel occlusion, 2VO) in rats. Since neuroinflammation, leading to neuronal apoptosis and death, is one of the mechanisms which is thought to play a significant role in chronic degenerative neurological disorders, the present study was planned to assess the neuroprotective role of ibuprofen in chronic cerebral hypoperfusion-induced neurodegeneration. After acclimatization, fifteen Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A – sham control, Group B – 2VO, and Group C – 2VO-I (treated daily with ibuprofen, 50 mg/kg, orally following 2VO). On 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal CA-1 region, hippocampal COX-2 mRNA expression and prostaglandin E2 (PGE-2) levels were estimated. There was a significant difference in neuronal cell death, increase in COX-2 mRNA expression and PGE-2 levels in 2VO group as compared to sham control group. In ibuprofen-treated 2VO (2VO-I) rats, the viable neuronal cell count of the hippocampal CA-1 region was significantly higher as compared to the untreated 2VO group. For the hippocampal COX-2 mRNA expression, the value in the ibuprofen treated group showed insignificant difference when compared to the untreated 2VO group. However, hippocampal PGE-2 levels were found to be significantly lower in the ibuprofen treated 2VO rats as compared to untreated 2VO rats. These results clearly indicate that ibuprofen is an effective neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats and can be fruitfully utilized in the management of Alzheimer’s disease.
format	Conference or Workshop Item
author	Saxena, Anil Kumar Mohd, Fadly Talib, Norlelawati A.
author_facet	Saxena, Anil Kumar Mohd, Fadly Talib, Norlelawati A.
author_sort	Saxena, Anil Kumar
title	Assessment of ibuprofen, non-selective cox inhibitor, as a neuroprotective agent in Alzheimer’s model of rats
title_short	Assessment of ibuprofen, non-selective cox inhibitor, as a neuroprotective agent in Alzheimer’s model of rats
title_full	Assessment of ibuprofen, non-selective cox inhibitor, as a neuroprotective agent in Alzheimer’s model of rats
title_fullStr	Assessment of ibuprofen, non-selective cox inhibitor, as a neuroprotective agent in Alzheimer’s model of rats
title_full_unstemmed	Assessment of ibuprofen, non-selective cox inhibitor, as a neuroprotective agent in Alzheimer’s model of rats
title_sort	assessment of ibuprofen, non-selective cox inhibitor, as a neuroprotective agent in alzheimer’s model of rats
publishDate	2013
url	http://irep.iium.edu.my/29564/ http://irep.iium.edu.my/29564/ http://irep.iium.edu.my/29564/1/IRIIE_POSTER_1.pdf http://irep.iium.edu.my/29564/4/Assessment_of_ibuprofen%2C_non-selective_cox_inhibitor%2C_as_a_neuroprotective_agent_in_Alzheimer%E2%80%99s_model_of_rats.pdf
first_indexed	2023-09-18T20:43:25Z
last_indexed	2023-09-18T20:43:25Z
_version_	1777409516701220864

Assessment of ibuprofen, non-selective cox inhibitor, as a neuroprotective agent in Alzheimer’s model of rats

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