Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice

Plants leaves of Coleus amboinicus from Indonesia (CAL-I) and from Malaysia, Coleus aromaticus (CAT-M), Pogostemon cablin (PC-M), Coleus blumei–red leaves (CBR-M), Coleus amboinicus– (CAL-M) Coleus blumei –purple leaves (CBP-M) were collected, freeze dried and extracted with aqueous methanol. The b...

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Main Authors:	Khattak, Muhammad Muzaffar Ali Khan, Bakhtiar, M. Taher, Abdul Rahman, Suzanah, Damanik , Rizal, Yahaya, Azhary
Format:	Conference or Workshop Item
Language:	English English
Published:	2012
Subjects:	TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/29733/ http://irep.iium.edu.my/29733/1/Muhammad_Muzaffar_Ali_Khan_Khattak-1.pdf http://irep.iium.edu.my/29733/2/MSTC_SCHEDULE.pdf

id	iium-29733
recordtype	eprints
spelling	iium-297332014-12-09T01:04:26Z http://irep.iium.edu.my/29733/ Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Abdul Rahman, Suzanah Damanik , Rizal Yahaya, Azhary TX341 Nutrition. Foods and food supply Plants leaves of Coleus amboinicus from Indonesia (CAL-I) and from Malaysia, Coleus aromaticus (CAT-M), Pogostemon cablin (PC-M), Coleus blumei–red leaves (CBR-M), Coleus amboinicus– (CAL-M) Coleus blumei –purple leaves (CBP-M) were collected, freeze dried and extracted with aqueous methanol. The biological activity in vitro, in relation to total phenolic, flavonoid & antioxidants contents and antimicrobial & antifungal activities were assessed. Total phenolics & antioxidants activities, antimicrobial & anti-fungal activities and toxicity were determined by using appropriate methods. The phenolic content among the extracts of leaves showed significant (p<0.05) difference ranging from 55.21 - 95.17 mg GAE/g of dried samples. The PC-M had the highest contents of phenolics followed by CBP-M. There were significant (p<0.05) differences in IC50 values ranging from 10.5 – 34.1 µg/ml. Among the species studied, CAL-M and PC-M showed higher antioxidants activities compared to the rest of the extracts. The extracts showed activities against bacteria and fungus and resulted in significant (p<0.05) differences between activities against microorganisms and fungus. The minimum inhibitory concentration of all leaves extracts ranged from 1.0–2.0 mg/ml in inhibiting the growth of S. aureus, E. coli, P. aeruginosa and B.subtilis. The acute toxicity test revealed that there was no mortality even at the highest doses levels used in mice i.e. 5000 mg/kg body weight. This study shows that the extracts can be used as antioxidants and antimicrobial agent without having any toxic effect. Furthermore, the anti- antimicrobial & anti-fungal activities relates to the concentration of total phenolic, flavonoid and antioxidants in the extracts. 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/29733/1/Muhammad_Muzaffar_Ali_Khan_Khattak-1.pdf application/pdf en http://irep.iium.edu.my/29733/2/MSTC_SCHEDULE.pdf Khattak, Muhammad Muzaffar Ali Khan and Bakhtiar, M. Taher and Abdul Rahman, Suzanah and Damanik , Rizal and Yahaya, Azhary (2012) Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice. In: Malaysian Sciences and Technology Congress 2012 , 20 November 2012. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TX341 Nutrition. Foods and food supply
spellingShingle	TX341 Nutrition. Foods and food supply Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Abdul Rahman, Suzanah Damanik , Rizal Yahaya, Azhary Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice
description	Plants leaves of Coleus amboinicus from Indonesia (CAL-I) and from Malaysia, Coleus aromaticus (CAT-M), Pogostemon cablin (PC-M), Coleus blumei–red leaves (CBR-M), Coleus amboinicus– (CAL-M) Coleus blumei –purple leaves (CBP-M) were collected, freeze dried and extracted with aqueous methanol. The biological activity in vitro, in relation to total phenolic, flavonoid & antioxidants contents and antimicrobial & antifungal activities were assessed. Total phenolics & antioxidants activities, antimicrobial & anti-fungal activities and toxicity were determined by using appropriate methods. The phenolic content among the extracts of leaves showed significant (p<0.05) difference ranging from 55.21 - 95.17 mg GAE/g of dried samples. The PC-M had the highest contents of phenolics followed by CBP-M. There were significant (p<0.05) differences in IC50 values ranging from 10.5 – 34.1 µg/ml. Among the species studied, CAL-M and PC-M showed higher antioxidants activities compared to the rest of the extracts. The extracts showed activities against bacteria and fungus and resulted in significant (p<0.05) differences between activities against microorganisms and fungus. The minimum inhibitory concentration of all leaves extracts ranged from 1.0–2.0 mg/ml in inhibiting the growth of S. aureus, E. coli, P. aeruginosa and B.subtilis. The acute toxicity test revealed that there was no mortality even at the highest doses levels used in mice i.e. 5000 mg/kg body weight. This study shows that the extracts can be used as antioxidants and antimicrobial agent without having any toxic effect. Furthermore, the anti- antimicrobial & anti-fungal activities relates to the concentration of total phenolic, flavonoid and antioxidants in the extracts.
format	Conference or Workshop Item
author	Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Abdul Rahman, Suzanah Damanik , Rizal Yahaya, Azhary
author_facet	Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Abdul Rahman, Suzanah Damanik , Rizal Yahaya, Azhary
author_sort	Khattak, Muhammad Muzaffar Ali Khan
title	Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice
title_short	Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice
title_full	Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice
title_fullStr	Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice
title_full_unstemmed	Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice
title_sort	coleus amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice
publishDate	2012
url	http://irep.iium.edu.my/29733/ http://irep.iium.edu.my/29733/1/Muhammad_Muzaffar_Ali_Khan_Khattak-1.pdf http://irep.iium.edu.my/29733/2/MSTC_SCHEDULE.pdf
first_indexed	2023-09-18T20:43:42Z
last_indexed	2023-09-18T20:43:42Z
_version_	1777409533779378176

Coleus Amboinicus exhibits anti-microbial and anti-fungus activities and having no toxicity at highest doses assessed in mice

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