Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land

Aims: This study was carried out to further characterize fungal species that could degrade 3-chloropropionic acid (3CP)as sole source of carbon and energy. Methodology and Results: Both fungi were able to grow on 3CP after 10 days on solid minimal media. Based on sequencing of its segment of 18S rR...

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Main Authors:	Parvizpour, Sepideh, Tengku Abdul Hamid, Tengku Haziyamin, Huyop, Fahrul Zaman
Format:	Article
Language:	English
Published:	Malaysian Society for Microbiology 2012
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/29815/ http://irep.iium.edu.my/29815/ http://irep.iium.edu.my/29815/1/Paper_UTM_Fungal_Sepideh.pdf

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recordtype	eprints
spelling	iium-298152014-11-27T08:42:49Z http://irep.iium.edu.my/29815/ Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land Parvizpour, Sepideh Tengku Abdul Hamid, Tengku Haziyamin Huyop, Fahrul Zaman Q Science (General) Aims: This study was carried out to further characterize fungal species that could degrade 3-chloropropionic acid (3CP)as sole source of carbon and energy. Methodology and Results: Both fungi were able to grow on 3CP after 10 days on solid minimal media. Based on sequencing of its segment of 18S rRNA these isolates were identified as Mucor sp. SP1 and Trichoderma sp. SP2. The isolated strains were not able to grow on media plates containing 10 mM of 2,2-dichloropropionate (2,2DCP) as sole source of carbon. 3CP degradation was observed in liquid minimal medium containing 10 mM 3CP after 18 days culture period. The chloride ion released was detected in both growth medium containing Mucor sp. SP1 and Trichoderma sp. SP2. At least 80% of 10 mM 3CP was utilized in the growth medium. Conclusion, significance and impact of study: Dehalogenase enzyme that can degrade α-chloro-substituted haloalkanoic acids for example 2,2DCP is well studied up to protein crystallization. Very few reports on the degradation of β-chloro-substituted haloalkanoic acids such as 3CP and none from fungi. This study is considered important because it can be compared to that of well-documented α-chloro-substituted haloalkanoic acids degradation. This is the first study to indicate fungal growth on 3CP as sole carbon and energy sources. Malaysian Society for Microbiology 2012-07-27 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29815/1/Paper_UTM_Fungal_Sepideh.pdf Parvizpour, Sepideh and Tengku Abdul Hamid, Tengku Haziyamin and Huyop, Fahrul Zaman (2012) Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land. Malaysian Journal of Microbiology, 9 (1). pp. 120-124. ISSN 1823-8262 (P), 2231-7538 (O) http://web.usm.my/mjm/issues/vol9/Short%203.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Parvizpour, Sepideh Tengku Abdul Hamid, Tengku Haziyamin Huyop, Fahrul Zaman Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land
description	Aims: This study was carried out to further characterize fungal species that could degrade 3-chloropropionic acid (3CP)as sole source of carbon and energy. Methodology and Results: Both fungi were able to grow on 3CP after 10 days on solid minimal media. Based on sequencing of its segment of 18S rRNA these isolates were identified as Mucor sp. SP1 and Trichoderma sp. SP2. The isolated strains were not able to grow on media plates containing 10 mM of 2,2-dichloropropionate (2,2DCP) as sole source of carbon. 3CP degradation was observed in liquid minimal medium containing 10 mM 3CP after 18 days culture period. The chloride ion released was detected in both growth medium containing Mucor sp. SP1 and Trichoderma sp. SP2. At least 80% of 10 mM 3CP was utilized in the growth medium. Conclusion, significance and impact of study: Dehalogenase enzyme that can degrade α-chloro-substituted haloalkanoic acids for example 2,2DCP is well studied up to protein crystallization. Very few reports on the degradation of β-chloro-substituted haloalkanoic acids such as 3CP and none from fungi. This study is considered important because it can be compared to that of well-documented α-chloro-substituted haloalkanoic acids degradation. This is the first study to indicate fungal growth on 3CP as sole carbon and energy sources.
format	Article
author	Parvizpour, Sepideh Tengku Abdul Hamid, Tengku Haziyamin Huyop, Fahrul Zaman
author_facet	Parvizpour, Sepideh Tengku Abdul Hamid, Tengku Haziyamin Huyop, Fahrul Zaman
author_sort	Parvizpour, Sepideh
title	Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land
title_short	Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land
title_full	Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land
title_fullStr	Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land
title_full_unstemmed	Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land
title_sort	molecular identification and biodegradation of 3-chloropropionic acid (3cp) by filamentous fungi-mucor and trichoderma species isolated from utm agricultural land
publisher	Malaysian Society for Microbiology
publishDate	2012
url	http://irep.iium.edu.my/29815/ http://irep.iium.edu.my/29815/ http://irep.iium.edu.my/29815/1/Paper_UTM_Fungal_Sepideh.pdf
first_indexed	2023-09-18T20:43:48Z
last_indexed	2023-09-18T20:43:48Z
_version_	1777409540501798912

Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land

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