Evaluation of antimicrobial activity exploration of the leaves extracts of Borreria articularis (Linn. F.)

In this study the plant of Borreria articularies (Linn. F) (family Rubiaceae) were subjected for phytochemical, antibacterial and antifungal evaluations. The sample powder was subjected to cold maceration by ethanol and methanol for 48 h at room temperature, concentrated by using a rotary vacuum...

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Main Authors:	Azad, Abdul Kalam, Wan Sulaiman, Wan Mohd. Azizi, Tengku Zakaria, Tengku Muhammad Faris
Format:	Article
Language:	English
Published:	Academic Journals 2013
Subjects:	QD Chemistry RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine
Online Access:	http://irep.iium.edu.my/30530/ http://irep.iium.edu.my/30530/ http://irep.iium.edu.my/30530/ http://irep.iium.edu.my/30530/1/Azad_et_al_%284%29_African_Journal_Biotecnology_2013.pdf

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spelling	iium-305302013-07-01T01:05:49Z http://irep.iium.edu.my/30530/ Evaluation of antimicrobial activity exploration of the leaves extracts of Borreria articularis (Linn. F.) Azad, Abdul Kalam Wan Sulaiman, Wan Mohd. Azizi Tengku Zakaria, Tengku Muhammad Faris QD Chemistry RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine In this study the plant of Borreria articularies (Linn. F) (family Rubiaceae) were subjected for phytochemical, antibacterial and antifungal evaluations. The sample powder was subjected to cold maceration by ethanol and methanol for 48 h at room temperature, concentrated by using a rotary vacuum evaporator (BUCHI R-205) and drying at −50 °C using bench top freeze dryer (ALPHA 1-4LD-2) and then kept in the fridge (4°C). Compound identification by comparing the NIST library data of the peaks with those reported in mass spectra of the peaks with literature data. The in vitro antimicrobial activities were determined by disc diffusion method and poisoned food technique respectively. Mueller- Hinton and Sabouraud medium was used for culture of bacteria and fungi. 5% ethanolic solution of the crude extracts and pure compound were used as the test material. The studies revealed that alkaloids, glycosides, tanins, flavonoids, saponins and reducing sugars are present in both the extracts. The pure compound, 6-methyl-5-cyclodecen-1-ol, exhibited good antimicrobial activity against all the pathogens tested. The highest zone of inhibition 20 mm was exhibited by ethanol and 6-methyl-5-cyclodecen-1-ol at a concentration of 2,000 μg/disc against both Escherichia coli and Vibrio cholera. The highest inhibition observed was 55.17 and 53.33% of fungal radial mycelial growth at a concentration of (100 μg/ml) against Aspergillus ustus and Aspergillus ochraceus respectively. The extracts exhibited antimicrobial activity which could serve as an alternative traditional medicine for treatment as antimicrobial agent. Keywords: Borreria articularis (Linn. F), 6-methyl 5-cyclodecen-1-ol, phytochemical screening, antimicrobial activity. Academic Journals 2013-06-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/30530/1/Azad_et_al_%284%29_African_Journal_Biotecnology_2013.pdf Azad, Abdul Kalam and Wan Sulaiman, Wan Mohd. Azizi and Tengku Zakaria, Tengku Muhammad Faris (2013) Evaluation of antimicrobial activity exploration of the leaves extracts of Borreria articularis (Linn. F.). African Journal of Biotechnology, 12 (23). pp. 3662-3668. ISSN 1684-5315 http://www.academicjournals.org/AJB/ 10.5897/AJB2013.12502
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine
spellingShingle	QD Chemistry RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine Azad, Abdul Kalam Wan Sulaiman, Wan Mohd. Azizi Tengku Zakaria, Tengku Muhammad Faris Evaluation of antimicrobial activity exploration of the leaves extracts of Borreria articularis (Linn. F.)
description	In this study the plant of Borreria articularies (Linn. F) (family Rubiaceae) were subjected for phytochemical, antibacterial and antifungal evaluations. The sample powder was subjected to cold maceration by ethanol and methanol for 48 h at room temperature, concentrated by using a rotary vacuum evaporator (BUCHI R-205) and drying at −50 °C using bench top freeze dryer (ALPHA 1-4LD-2) and then kept in the fridge (4°C). Compound identification by comparing the NIST library data of the peaks with those reported in mass spectra of the peaks with literature data. The in vitro antimicrobial activities were determined by disc diffusion method and poisoned food technique respectively. Mueller- Hinton and Sabouraud medium was used for culture of bacteria and fungi. 5% ethanolic solution of the crude extracts and pure compound were used as the test material. The studies revealed that alkaloids, glycosides, tanins, flavonoids, saponins and reducing sugars are present in both the extracts. The pure compound, 6-methyl-5-cyclodecen-1-ol, exhibited good antimicrobial activity against all the pathogens tested. The highest zone of inhibition 20 mm was exhibited by ethanol and 6-methyl-5-cyclodecen-1-ol at a concentration of 2,000 μg/disc against both Escherichia coli and Vibrio cholera. The highest inhibition observed was 55.17 and 53.33% of fungal radial mycelial growth at a concentration of (100 μg/ml) against Aspergillus ustus and Aspergillus ochraceus respectively. The extracts exhibited antimicrobial activity which could serve as an alternative traditional medicine for treatment as antimicrobial agent. Keywords: Borreria articularis (Linn. F), 6-methyl 5-cyclodecen-1-ol, phytochemical screening, antimicrobial activity.
format	Article
author	Azad, Abdul Kalam Wan Sulaiman, Wan Mohd. Azizi Tengku Zakaria, Tengku Muhammad Faris
author_facet	Azad, Abdul Kalam Wan Sulaiman, Wan Mohd. Azizi Tengku Zakaria, Tengku Muhammad Faris
author_sort	Azad, Abdul Kalam
title	Evaluation of antimicrobial activity exploration of the leaves extracts of Borreria articularis (Linn. F.)
title_short	Evaluation of antimicrobial activity exploration of the leaves extracts of Borreria articularis (Linn. F.)
title_full	Evaluation of antimicrobial activity exploration of the leaves extracts of Borreria articularis (Linn. F.)
title_fullStr	Evaluation of antimicrobial activity exploration of the leaves extracts of Borreria articularis (Linn. F.)
title_full_unstemmed	Evaluation of antimicrobial activity exploration of the leaves extracts of Borreria articularis (Linn. F.)
title_sort	evaluation of antimicrobial activity exploration of the leaves extracts of borreria articularis (linn. f.)
publisher	Academic Journals
publishDate	2013
url	http://irep.iium.edu.my/30530/ http://irep.iium.edu.my/30530/ http://irep.iium.edu.my/30530/ http://irep.iium.edu.my/30530/1/Azad_et_al_%284%29_African_Journal_Biotecnology_2013.pdf
first_indexed	2023-09-18T20:44:42Z
last_indexed	2023-09-18T20:44:42Z
_version_	1777409597149020160

Evaluation of antimicrobial activity exploration of the leaves extracts of Borreria articularis (Linn. F.)

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