Isolation of pigment-producing strain of Staphylococcus kloosii from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) from Malaysian waters
Staphylococcus kloosii, an orange pigment-producing bacterium, was isolated from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt 1835) from Teluk Nipah, Pangkor Island, Perak, Malaysia. This report is the first documentation of this Gram-positive strain, referred to a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Sains Malaysia
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/30575/ http://irep.iium.edu.my/30575/ http://irep.iium.edu.my/30575/4/2013_TLSR_Kamarudin_et_al__Staphylococcus_kloosii_pigment.pdf |
| Summary: | Staphylococcus kloosii, an orange pigment-producing bacterium, was isolated
from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt 1835) from
Teluk Nipah, Pangkor Island, Perak, Malaysia. This report is the first documentation of this
Gram-positive strain, referred to as Strain 68 in Malaysia. A partial 16S ribosomal RNA
gene sequence of the mesophilic strain has been registered with GenBank (National
Center for Biotechnology Information, US National Library of Medicine) with accession
number JX102547. Phylogenetic analysis using the neighbour-joining method further
supported the identification of Strain 68 as S. kloosii. The circular strain produced orange
pigments on tryptone glucose yeast extract agar (TGYEA) and in nutrient broth (NB) at
approximately pH 7. The visible spectra of ethanolic and methanolic pigment extracts of
the bacterial strain were considered identical with λmax at 426, 447 and 475 nm and λmax at
426, 445 and 473 nm, respectively. Both visible spectra resemble the visible spectra of
lutein, which is a commercial carotenoid; however, further analyses are required to confirm the identity of this pigment. The methanolic extracts of the intracellular pigments
comprised at least three pigment compounds: an orange pigment compound (major
compound), a yellow pigment compound (the least polar) and a pink pigment compound
(the most polar). These findings are the first documentation of the pigment composition of
S. kloosii as no such record could be found to date. |
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