Isolation of pigment-producing strain of Staphylococcus kloosii from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) from Malaysian waters

Staphylococcus kloosii, an orange pigment-producing bacterium, was isolated from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt 1835) from Teluk Nipah, Pangkor Island, Perak, Malaysia. This report is the first documentation of this Gram-positive strain, referred to a...

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Main Authors:	Kamarudin, Kamarul Rahim, Ngah, Nurziana, Tengku Abdul Hamid, Tengku Haziyamin, Darnis, Deny Susanti
Format:	Article
Language:	English
Published:	Penerbit Universiti Sains Malaysia 2013
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/30575/ http://irep.iium.edu.my/30575/ http://irep.iium.edu.my/30575/4/2013_TLSR_Kamarudin_et_al__Staphylococcus_kloosii_pigment.pdf

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spelling	iium-305752013-08-22T00:12:42Z http://irep.iium.edu.my/30575/ Isolation of pigment-producing strain of Staphylococcus kloosii from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) from Malaysian waters Kamarudin, Kamarul Rahim Ngah, Nurziana Tengku Abdul Hamid, Tengku Haziyamin Darnis, Deny Susanti QR Microbiology Staphylococcus kloosii, an orange pigment-producing bacterium, was isolated from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt 1835) from Teluk Nipah, Pangkor Island, Perak, Malaysia. This report is the first documentation of this Gram-positive strain, referred to as Strain 68 in Malaysia. A partial 16S ribosomal RNA gene sequence of the mesophilic strain has been registered with GenBank (National Center for Biotechnology Information, US National Library of Medicine) with accession number JX102547. Phylogenetic analysis using the neighbour-joining method further supported the identification of Strain 68 as S. kloosii. The circular strain produced orange pigments on tryptone glucose yeast extract agar (TGYEA) and in nutrient broth (NB) at approximately pH 7. The visible spectra of ethanolic and methanolic pigment extracts of the bacterial strain were considered identical with λmax at 426, 447 and 475 nm and λmax at 426, 445 and 473 nm, respectively. Both visible spectra resemble the visible spectra of lutein, which is a commercial carotenoid; however, further analyses are required to confirm the identity of this pigment. The methanolic extracts of the intracellular pigments comprised at least three pigment compounds: an orange pigment compound (major compound), a yellow pigment compound (the least polar) and a pink pigment compound (the most polar). These findings are the first documentation of the pigment composition of S. kloosii as no such record could be found to date. Penerbit Universiti Sains Malaysia 2013-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/30575/4/2013_TLSR_Kamarudin_et_al__Staphylococcus_kloosii_pigment.pdf Kamarudin, Kamarul Rahim and Ngah, Nurziana and Tengku Abdul Hamid, Tengku Haziyamin and Darnis, Deny Susanti (2013) Isolation of pigment-producing strain of Staphylococcus kloosii from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) from Malaysian waters. Tropical Life Sciences Research, 24 (1). pp. 85-100. ISSN 1985-3718 http://www.tlsr.usm.my/abstract.asp?kod_title=776
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Kamarudin, Kamarul Rahim Ngah, Nurziana Tengku Abdul Hamid, Tengku Haziyamin Darnis, Deny Susanti Isolation of pigment-producing strain of Staphylococcus kloosii from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) from Malaysian waters
description	Staphylococcus kloosii, an orange pigment-producing bacterium, was isolated from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt 1835) from Teluk Nipah, Pangkor Island, Perak, Malaysia. This report is the first documentation of this Gram-positive strain, referred to as Strain 68 in Malaysia. A partial 16S ribosomal RNA gene sequence of the mesophilic strain has been registered with GenBank (National Center for Biotechnology Information, US National Library of Medicine) with accession number JX102547. Phylogenetic analysis using the neighbour-joining method further supported the identification of Strain 68 as S. kloosii. The circular strain produced orange pigments on tryptone glucose yeast extract agar (TGYEA) and in nutrient broth (NB) at approximately pH 7. The visible spectra of ethanolic and methanolic pigment extracts of the bacterial strain were considered identical with λmax at 426, 447 and 475 nm and λmax at 426, 445 and 473 nm, respectively. Both visible spectra resemble the visible spectra of lutein, which is a commercial carotenoid; however, further analyses are required to confirm the identity of this pigment. The methanolic extracts of the intracellular pigments comprised at least three pigment compounds: an orange pigment compound (major compound), a yellow pigment compound (the least polar) and a pink pigment compound (the most polar). These findings are the first documentation of the pigment composition of S. kloosii as no such record could be found to date.
format	Article
author	Kamarudin, Kamarul Rahim Ngah, Nurziana Tengku Abdul Hamid, Tengku Haziyamin Darnis, Deny Susanti
author_facet	Kamarudin, Kamarul Rahim Ngah, Nurziana Tengku Abdul Hamid, Tengku Haziyamin Darnis, Deny Susanti
author_sort	Kamarudin, Kamarul Rahim
title	Isolation of pigment-producing strain of Staphylococcus kloosii from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) from Malaysian waters
title_short	Isolation of pigment-producing strain of Staphylococcus kloosii from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) from Malaysian waters
title_full	Isolation of pigment-producing strain of Staphylococcus kloosii from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) from Malaysian waters
title_fullStr	Isolation of pigment-producing strain of Staphylococcus kloosii from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) from Malaysian waters
title_full_unstemmed	Isolation of pigment-producing strain of Staphylococcus kloosii from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) from Malaysian waters
title_sort	isolation of pigment-producing strain of staphylococcus kloosii from the respiratory tree of holothuria (mertensiothuria) leucospilota (brandt, 1835) from malaysian waters
publisher	Penerbit Universiti Sains Malaysia
publishDate	2013
url	http://irep.iium.edu.my/30575/ http://irep.iium.edu.my/30575/ http://irep.iium.edu.my/30575/4/2013_TLSR_Kamarudin_et_al__Staphylococcus_kloosii_pigment.pdf
first_indexed	2023-09-18T20:44:46Z
last_indexed	2023-09-18T20:44:46Z
_version_	1777409600945913856

Isolation of pigment-producing strain of Staphylococcus kloosii from the respiratory tree of Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) from Malaysian waters

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