A rapid and highly efficient method for transformation of sugarcane callus

Modern sugarcane cultivars have complex genetic characteristics and low fertility that render their genetic improvement through traditional breeding difficult. Genetic engineering methodology to introduce foreign genes provides new opportunities for the genetic improvement of sugarcane cultivars. On...

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Main Authors:	Santosa, Dwi Andreas, Hendroko, Roy, Farouk, Abdelazim, Greiner, Ralf
Format:	Article
Language:	English
Published:	Humana Press 2004
Subjects:	TA Engineering (General). Civil engineering (General)
Online Access:	http://irep.iium.edu.my/30844/ http://irep.iium.edu.my/30844/ http://irep.iium.edu.my/30844/ http://irep.iium.edu.my/30844/1/A_rapid_and_highly.pdf

id	iium-30844
recordtype	eprints
spelling	iium-308442017-06-22T04:45:44Z http://irep.iium.edu.my/30844/ A rapid and highly efficient method for transformation of sugarcane callus Santosa, Dwi Andreas Hendroko, Roy Farouk, Abdelazim Greiner, Ralf TA Engineering (General). Civil engineering (General) Modern sugarcane cultivars have complex genetic characteristics and low fertility that render their genetic improvement through traditional breeding difficult. Genetic engineering methodology to introduce foreign genes provides new opportunities for the genetic improvement of sugarcane cultivars. One of prerequisites for successful insertion of a gene cassette into the plant genome is the availability of an efficient transformation protocol. An improved protocol for Agrobacterium-mediated transformation of sugarcane is described. Between 85 and 100% of calli transformed using this procedure produced new calli, and 100% of them were positive for the inserted gene. The whole procedure permitted the production of transgenic calli in a short time (1.5 mo). The transformed calli can be cultured further for the production of the inserted gene-encoded enzyme by using cell culture, or they can be regenerated into transgenic plants. This protocol may be implemented also for the generation of transgenic plants from other species. Humana Press 2004-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/30844/1/A_rapid_and_highly.pdf Santosa, Dwi Andreas and Hendroko, Roy and Farouk, Abdelazim and Greiner, Ralf (2004) A rapid and highly efficient method for transformation of sugarcane callus. Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology, 28 (2). pp. 113-119. ISSN 1073-6085 E-ISSN 1559-0305 http://repository.ipb.ac.id/handle/123456789/28102 10.1385/MB:28:2:113
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA Engineering (General). Civil engineering (General)
spellingShingle	TA Engineering (General). Civil engineering (General) Santosa, Dwi Andreas Hendroko, Roy Farouk, Abdelazim Greiner, Ralf A rapid and highly efficient method for transformation of sugarcane callus
description	Modern sugarcane cultivars have complex genetic characteristics and low fertility that render their genetic improvement through traditional breeding difficult. Genetic engineering methodology to introduce foreign genes provides new opportunities for the genetic improvement of sugarcane cultivars. One of prerequisites for successful insertion of a gene cassette into the plant genome is the availability of an efficient transformation protocol. An improved protocol for Agrobacterium-mediated transformation of sugarcane is described. Between 85 and 100% of calli transformed using this procedure produced new calli, and 100% of them were positive for the inserted gene. The whole procedure permitted the production of transgenic calli in a short time (1.5 mo). The transformed calli can be cultured further for the production of the inserted gene-encoded enzyme by using cell culture, or they can be regenerated into transgenic plants. This protocol may be implemented also for the generation of transgenic plants from other species.
format	Article
author	Santosa, Dwi Andreas Hendroko, Roy Farouk, Abdelazim Greiner, Ralf
author_facet	Santosa, Dwi Andreas Hendroko, Roy Farouk, Abdelazim Greiner, Ralf
author_sort	Santosa, Dwi Andreas
title	A rapid and highly efficient method for transformation of sugarcane callus
title_short	A rapid and highly efficient method for transformation of sugarcane callus
title_full	A rapid and highly efficient method for transformation of sugarcane callus
title_fullStr	A rapid and highly efficient method for transformation of sugarcane callus
title_full_unstemmed	A rapid and highly efficient method for transformation of sugarcane callus
title_sort	rapid and highly efficient method for transformation of sugarcane callus
publisher	Humana Press
publishDate	2004
url	http://irep.iium.edu.my/30844/ http://irep.iium.edu.my/30844/ http://irep.iium.edu.my/30844/ http://irep.iium.edu.my/30844/1/A_rapid_and_highly.pdf
first_indexed	2023-09-18T20:45:06Z
last_indexed	2023-09-18T20:45:06Z
_version_	1777409621912190976

A rapid and highly efficient method for transformation of sugarcane callus

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