Response of Arabidopsis thaliana seedlings to lead exposure: effect of pre-treatment with sodium nitroprusside

Plants are regularly exposed to unfavorable conditions that impose stress. Lead (Pb), is one of the major pollutants in the environment that causes serious public health and environmental concerns. Increasing levels of lead could also have severe consequences for plants. Plants exposed to lead st...

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Main Authors:	Phang, Ing Chia, Leung, David W. M., Taylor, H. Harry, Burritt, David J.
Format:	Conference or Workshop Item
Language:	English English
Published:	2009
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/310/ http://irep.iium.edu.my/310/ http://irep.iium.edu.my/310/1/CONFERENCE_PROGRAM.pdf http://irep.iium.edu.my/310/2/Abstract_-_IngChia.pdf

id	iium-310
recordtype	eprints
spelling	iium-3102011-11-16T06:21:11Z http://irep.iium.edu.my/310/ Response of Arabidopsis thaliana seedlings to lead exposure: effect of pre-treatment with sodium nitroprusside Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J. Q Science (General) Plants are regularly exposed to unfavorable conditions that impose stress. Lead (Pb), is one of the major pollutants in the environment that causes serious public health and environmental concerns. Increasing levels of lead could also have severe consequences for plants. Plants exposed to lead stress initiate signaling pathways, and make specific changes in their cell physiology and metabolism to avoid or tolerate the stress. However, mechanisms to reduce the effects of lead may vary from plant to plant, developmental stage, and culture medium. Nitric oxide is an important signaling molecule in plant development and defense responses. It has been shown to play a major role in plant responses to several abiotic stresses, such as heat, chilling, drought, salt, UV irradiation and ozone exposure. The objective of this study is to examine the physiological responses to Pb exposure of Arabidopsis thaliana seeds pre-treated with sodium nitroprusside (SNP), a nitric oxide donor. All experiments were carried out using 7-day-old seedlings in a laboratory environment. Pb contents were determined using a graphite furnace spectrometer. Antioxidant assays and reactive oxygen species were carried out using a microplate reader. A major finding is that Pb treatment resulted in increased oxidative stress, which was counteracted by SNP pre-treatment. This and other results obtained are discussed in relation to a strategy to tolerate Pb accumulation in plant cells. 2009-09-20 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/310/1/CONFERENCE_PROGRAM.pdf application/pdf en http://irep.iium.edu.my/310/2/Abstract_-_IngChia.pdf Phang, Ing Chia and Leung, David W. M. and Taylor, H. Harry and Burritt, David J. (2009) Response of Arabidopsis thaliana seedlings to lead exposure: effect of pre-treatment with sodium nitroprusside. In: 13th Australasian Society for Ecotoxicology Conference, 20-23 September 2009, Adelaide, South Australia. http://www.clw.csiro.au/conferences/ase/posters.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J. Response of Arabidopsis thaliana seedlings to lead exposure: effect of pre-treatment with sodium nitroprusside
description	Plants are regularly exposed to unfavorable conditions that impose stress. Lead (Pb), is one of the major pollutants in the environment that causes serious public health and environmental concerns. Increasing levels of lead could also have severe consequences for plants. Plants exposed to lead stress initiate signaling pathways, and make specific changes in their cell physiology and metabolism to avoid or tolerate the stress. However, mechanisms to reduce the effects of lead may vary from plant to plant, developmental stage, and culture medium. Nitric oxide is an important signaling molecule in plant development and defense responses. It has been shown to play a major role in plant responses to several abiotic stresses, such as heat, chilling, drought, salt, UV irradiation and ozone exposure. The objective of this study is to examine the physiological responses to Pb exposure of Arabidopsis thaliana seeds pre-treated with sodium nitroprusside (SNP), a nitric oxide donor. All experiments were carried out using 7-day-old seedlings in a laboratory environment. Pb contents were determined using a graphite furnace spectrometer. Antioxidant assays and reactive oxygen species were carried out using a microplate reader. A major finding is that Pb treatment resulted in increased oxidative stress, which was counteracted by SNP pre-treatment. This and other results obtained are discussed in relation to a strategy to tolerate Pb accumulation in plant cells.
format	Conference or Workshop Item
author	Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J.
author_facet	Phang, Ing Chia Leung, David W. M. Taylor, H. Harry Burritt, David J.
author_sort	Phang, Ing Chia
title	Response of Arabidopsis thaliana seedlings to lead exposure: effect of pre-treatment with sodium nitroprusside
title_short	Response of Arabidopsis thaliana seedlings to lead exposure: effect of pre-treatment with sodium nitroprusside
title_full	Response of Arabidopsis thaliana seedlings to lead exposure: effect of pre-treatment with sodium nitroprusside
title_fullStr	Response of Arabidopsis thaliana seedlings to lead exposure: effect of pre-treatment with sodium nitroprusside
title_full_unstemmed	Response of Arabidopsis thaliana seedlings to lead exposure: effect of pre-treatment with sodium nitroprusside
title_sort	response of arabidopsis thaliana seedlings to lead exposure: effect of pre-treatment with sodium nitroprusside
publishDate	2009
url	http://irep.iium.edu.my/310/ http://irep.iium.edu.my/310/ http://irep.iium.edu.my/310/1/CONFERENCE_PROGRAM.pdf http://irep.iium.edu.my/310/2/Abstract_-_IngChia.pdf
first_indexed	2023-09-18T20:07:26Z
last_indexed	2023-09-18T20:07:26Z
_version_	1777407252909522944

Response of Arabidopsis thaliana seedlings to lead exposure: effect of pre-treatment with sodium nitroprusside

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