Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients

Loss of heterozygosity (LOH) on several loci and mutations on PTEN tumor suppressor gene (10q23.3) occur frequently in sporadic gliomas. We have performed polymerase chain reaction (PCR)-LOH analysis using microsatellite markers and single-stranded conformational polymorphism (SSCP) analysis to dete...

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Main Authors:	Zainuddin, Norafiza, Jaafar, Hasnan, Isa, Mohd. Nizam, Abdullah, Jafri Malin
Format:	Article
Language:	English
Published:	Malaysian Medical Associations 2004
Subjects:	RC0254 Neoplasms. Tumors. Oncology (including Cancer) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Online Access:	http://irep.iium.edu.my/31130/ http://irep.iium.edu.my/31130/ http://irep.iium.edu.my/31130/1/MJM_Malignant_Gliomas.pdf

id	iium-31130
recordtype	eprints
spelling	iium-311302013-08-13T02:39:05Z http://irep.iium.edu.my/31130/ Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin RC0254 Neoplasms. Tumors. Oncology (including Cancer) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Loss of heterozygosity (LOH) on several loci and mutations on PTEN tumor suppressor gene (10q23.3) occur frequently in sporadic gliomas. We have performed polymerase chain reaction (PCR)-LOH analysis using microsatellite markers and single-stranded conformational polymorphism (SSCP) analysis to determine the incidence of allelic losses on chromosome 10q, 9p, 17p and 13q and mutations of exons 5, 6 and 8 of the PTEN gene in malignant gliomas. Twelve of 23 (52.2%) malignant glioma cases showed allelic losses whereas 7 of 23 (30.4%) samples showed aberrant band patterns and mutations of the PTEN gene. Four of these cases showed LOH on 10q23 and mutations of the PTEN gene. The data on LOH indicated the involvement of different genes in gliomagenesis whereas mutations of the PTEN gene indicated the role of PTEN tumor suppressor gene in the progression of glioma in Malay population. Malaysian Medical Associations 2004-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/31130/1/MJM_Malignant_Gliomas.pdf Zainuddin, Norafiza and Jaafar, Hasnan and Isa, Mohd. Nizam and Abdullah, Jafri Malin (2004) Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients. Medical Journal of Malaysia, 59 (4). pp. 468-479. ISSN 0300-5283 http://www.mma.org.my/Publications/MedicalJournalofMalaysia/tabid/69/Default.aspx
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC0254 Neoplasms. Tumors. Oncology (including Cancer) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
spellingShingle	RC0254 Neoplasms. Tumors. Oncology (including Cancer) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients
description	Loss of heterozygosity (LOH) on several loci and mutations on PTEN tumor suppressor gene (10q23.3) occur frequently in sporadic gliomas. We have performed polymerase chain reaction (PCR)-LOH analysis using microsatellite markers and single-stranded conformational polymorphism (SSCP) analysis to determine the incidence of allelic losses on chromosome 10q, 9p, 17p and 13q and mutations of exons 5, 6 and 8 of the PTEN gene in malignant gliomas. Twelve of 23 (52.2%) malignant glioma cases showed allelic losses whereas 7 of 23 (30.4%) samples showed aberrant band patterns and mutations of the PTEN gene. Four of these cases showed LOH on 10q23 and mutations of the PTEN gene. The data on LOH indicated the involvement of different genes in gliomagenesis whereas mutations of the PTEN gene indicated the role of PTEN tumor suppressor gene in the progression of glioma in Malay population.
format	Article
author	Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin
author_facet	Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin
author_sort	Zainuddin, Norafiza
title	Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients
title_short	Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients
title_full	Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients
title_fullStr	Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients
title_full_unstemmed	Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients
title_sort	presence of allelic loss and pten mutations in malignant gliomas from malay patients
publisher	Malaysian Medical Associations
publishDate	2004
url	http://irep.iium.edu.my/31130/ http://irep.iium.edu.my/31130/ http://irep.iium.edu.my/31130/1/MJM_Malignant_Gliomas.pdf
first_indexed	2023-09-18T20:45:22Z
last_indexed	2023-09-18T20:45:22Z
_version_	1777409639592230912

Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients

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