Investigation of effects of different media on CHO cell growth and production of IGFI
Different media is formulated with varied concentration of nutrients resulting in different cell growth behaviour and productivity of the system. The objective of the study was therefore to investigate the effects of different media on CHO-K1 cell growth and production of Insulin-like Growth Factor...
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| Format: | Monograph |
| Language: | English English |
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2012
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| Online Access: | http://irep.iium.edu.my/31214/ http://irep.iium.edu.my/31214/1/Full_report_EDWB10-111-0450_%28Yumi%29.pdf http://irep.iium.edu.my/31214/2/End_of_Project_Report_EDWB10-111-0450%28Yumi%29.pdf |
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iium-312142013-09-30T06:00:04Z http://irep.iium.edu.my/31214/ Investigation of effects of different media on CHO cell growth and production of IGFI Hashim, Yumi Zuhanis Has-Yun T Technology (General) Different media is formulated with varied concentration of nutrients resulting in different cell growth behaviour and productivity of the system. The objective of the study was therefore to investigate the effects of different media on CHO-K1 cell growth and production of Insulin-like Growth Factor-1 (IGF-1) towards identifying the optimal media for further use. CHO-K1 were cultured in Ham's 12 and RPMI media respectively. Samples were collected at designated time intervals for cell counting, biochemical analysis and determination of biomass, total protein as well as IGF-1 concentration. Both univariate and multivariate data analysis (MVDA) approach were used to analyse data. Using univariate analysis, RPMI gave higher saturation density (9.13 x 10^5 cells/ml) and IGF-1 (31.56 pg/ml) concentration when compared to Ham's 12 (saturation density: 8.65 x 10^5 cells/ml; IGF-1: 29.87 pg/ml). Meanwhile MVDA showed that culture was clearly separated by types of media. Interestingly, glucose utilization was shown to possibly cause the separation rather than cell number related observations. Taken together, RPMI was suggested as a better choice for CHO-K1 culture since it enabled the culture to efficiently consume glucose to yield high cell saturation density and IGF-1. In addition, glutamine and IGF-1 was observed to have positive correlation which warrants further research. [s.n] 2012-05-16 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/31214/1/Full_report_EDWB10-111-0450_%28Yumi%29.pdf application/pdf en http://irep.iium.edu.my/31214/2/End_of_Project_Report_EDWB10-111-0450%28Yumi%29.pdf Hashim, Yumi Zuhanis Has-Yun (2012) Investigation of effects of different media on CHO cell growth and production of IGFI. Research Report. [s.n]. (Unpublished) |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
T Technology (General) |
| spellingShingle |
T Technology (General) Hashim, Yumi Zuhanis Has-Yun Investigation of effects of different media on CHO cell growth and production of IGFI |
| description |
Different media is formulated with varied concentration of nutrients resulting in different cell growth behaviour and productivity of the system. The objective of the study was therefore to investigate the effects of different media on CHO-K1 cell growth and production of Insulin-like Growth Factor-1 (IGF-1) towards identifying the optimal media for further use. CHO-K1 were cultured in Ham's 12 and RPMI media respectively. Samples were collected at designated time intervals for cell counting, biochemical analysis and determination of biomass, total protein as well as IGF-1 concentration. Both univariate and multivariate data analysis (MVDA) approach were used to analyse data. Using univariate analysis, RPMI gave higher saturation density (9.13 x 10^5 cells/ml) and IGF-1 (31.56 pg/ml) concentration when compared to Ham's 12 (saturation density: 8.65 x 10^5 cells/ml; IGF-1: 29.87 pg/ml). Meanwhile MVDA showed that culture was clearly separated by types of media. Interestingly, glucose utilization was shown to possibly cause the separation rather than cell number related observations. Taken together, RPMI was suggested as a better choice for CHO-K1 culture since it enabled the culture to efficiently consume glucose to yield high cell saturation density and IGF-1. In addition, glutamine and IGF-1 was observed to have positive correlation which warrants further research.
|
| format |
Monograph |
| author |
Hashim, Yumi Zuhanis Has-Yun |
| author_facet |
Hashim, Yumi Zuhanis Has-Yun |
| author_sort |
Hashim, Yumi Zuhanis Has-Yun |
| title |
Investigation of effects of different media on CHO cell growth and production of IGFI |
| title_short |
Investigation of effects of different media on CHO cell growth and production of IGFI |
| title_full |
Investigation of effects of different media on CHO cell growth and production of IGFI |
| title_fullStr |
Investigation of effects of different media on CHO cell growth and production of IGFI |
| title_full_unstemmed |
Investigation of effects of different media on CHO cell growth and production of IGFI |
| title_sort |
investigation of effects of different media on cho cell growth and production of igfi |
| publisher |
[s.n] |
| publishDate |
2012 |
| url |
http://irep.iium.edu.my/31214/ http://irep.iium.edu.my/31214/1/Full_report_EDWB10-111-0450_%28Yumi%29.pdf http://irep.iium.edu.my/31214/2/End_of_Project_Report_EDWB10-111-0450%28Yumi%29.pdf |
| first_indexed |
2023-09-18T20:45:27Z |
| last_indexed |
2023-09-18T20:45:27Z |
| _version_ |
1777409644725010432 |