LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma

A rapid, simple, sensitive and specific liquid chromatography–tandem mass spectrometry method was developed and validated for simultaneous quantification of itopride hydrochloride and domperidone in human plasma. Both drugs were extracted by liquid–liquid extraction with ethyl acetate and saturated...

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Main Authors:	Bose, Anirbandeep, Bhaumik, Uttam, Ghosh, Animesh, Chatterjee, Bappaditya, Chakraborty, Uday Sankar, Sarkar, Amlan Kanti, Pal, Tapan Kumar
Format:	Article
Language:	English
Published:	Vieweg Verlag 2009
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/31912/ http://irep.iium.edu.my/31912/ http://irep.iium.edu.my/31912/ http://irep.iium.edu.my/31912/1/Paper8.pdf

id	iium-31912
recordtype	eprints
spelling	iium-319122013-09-20T06:04:41Z http://irep.iium.edu.my/31912/ LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma Bose, Anirbandeep Bhaumik, Uttam Ghosh, Animesh Chatterjee, Bappaditya Chakraborty, Uday Sankar Sarkar, Amlan Kanti Pal, Tapan Kumar R Medicine (General) A rapid, simple, sensitive and specific liquid chromatography–tandem mass spectrometry method was developed and validated for simultaneous quantification of itopride hydrochloride and domperidone in human plasma. Both drugs were extracted by liquid–liquid extraction with ethyl acetate and saturated borax solution. The chromatographic separation was performed on a reversed-phase C18 column with a mobile phase of water–methanol (2:98, v/v) containing 0.5% formic acid. The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The assay exhibited linearity over the concentration range of 3.33–500 ng mL−1 for itopride hydrochloride and 3.33–100 ng mL−1 for domperidone in human plasma. The precursor to product ion transitions of m/z 359.1–72.3 and 426.0–147.2 were used to measure itopride hydrochloride and domperidone respectively. The method was found suitable for the analysis of plasma samples collected during phase 1 pharmacokinetics study of itopride HCl 50 mg and domperidone 20 mg in 12 healthy volunteers after single oral doses of the combination drug. Vieweg Verlag 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/31912/1/Paper8.pdf Bose, Anirbandeep and Bhaumik, Uttam and Ghosh, Animesh and Chatterjee, Bappaditya and Chakraborty, Uday Sankar and Sarkar, Amlan Kanti and Pal, Tapan Kumar (2009) LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma. Chromatographia, 69 (11/12). pp. 1233-1241. ISSN 1612-1112 (O), 0009-5893 (P) http://link.springer.com/article/10.1365%2Fs10337-009-1032-0 10.1365/s10337-009-1032-0
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Bose, Anirbandeep Bhaumik, Uttam Ghosh, Animesh Chatterjee, Bappaditya Chakraborty, Uday Sankar Sarkar, Amlan Kanti Pal, Tapan Kumar LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma
description	A rapid, simple, sensitive and specific liquid chromatography–tandem mass spectrometry method was developed and validated for simultaneous quantification of itopride hydrochloride and domperidone in human plasma. Both drugs were extracted by liquid–liquid extraction with ethyl acetate and saturated borax solution. The chromatographic separation was performed on a reversed-phase C18 column with a mobile phase of water–methanol (2:98, v/v) containing 0.5% formic acid. The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The assay exhibited linearity over the concentration range of 3.33–500 ng mL−1 for itopride hydrochloride and 3.33–100 ng mL−1 for domperidone in human plasma. The precursor to product ion transitions of m/z 359.1–72.3 and 426.0–147.2 were used to measure itopride hydrochloride and domperidone respectively. The method was found suitable for the analysis of plasma samples collected during phase 1 pharmacokinetics study of itopride HCl 50 mg and domperidone 20 mg in 12 healthy volunteers after single oral doses of the combination drug.
format	Article
author	Bose, Anirbandeep Bhaumik, Uttam Ghosh, Animesh Chatterjee, Bappaditya Chakraborty, Uday Sankar Sarkar, Amlan Kanti Pal, Tapan Kumar
author_facet	Bose, Anirbandeep Bhaumik, Uttam Ghosh, Animesh Chatterjee, Bappaditya Chakraborty, Uday Sankar Sarkar, Amlan Kanti Pal, Tapan Kumar
author_sort	Bose, Anirbandeep
title	LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma
title_short	LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma
title_full	LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma
title_fullStr	LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma
title_full_unstemmed	LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma
title_sort	lc-ms/ms simulatneous determination of itopride hydrochloride and domperidone in human plasma
publisher	Vieweg Verlag
publishDate	2009
url	http://irep.iium.edu.my/31912/ http://irep.iium.edu.my/31912/ http://irep.iium.edu.my/31912/ http://irep.iium.edu.my/31912/1/Paper8.pdf
first_indexed	2023-09-18T20:46:03Z
last_indexed	2023-09-18T20:46:03Z
_version_	1777409682393006080

LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma

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