Development and validation of a high-performance liquid chromatographic method for bioanalytical application with rimonabant
A simple and feasible high-performance liquid chromatographic method with UV detection was developed and validated for the quantification of rimonabant in human plasma. The chromatographic separation was carried out in a Hypersil BDS, C18 column (250 mm×4.6 mm; 5�m). The mobile phase was a mixture...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/31917/ http://irep.iium.edu.my/31917/ http://irep.iium.edu.my/31917/ http://irep.iium.edu.my/31917/1/Paper10.pdf |
| Summary: | A simple and feasible high-performance liquid chromatographic method with UV detection was developed and validated for the quantification of rimonabant in human plasma. The chromatographic
separation was carried out in a Hypersil BDS, C18 column (250 mm×4.6 mm; 5�m). The mobile phase
was a mixture of 10 mM phosphate buffer and acetonitrile (30:70, v/v) at a flow rate of 1.0 ml/min. The
UV detection was set at 220 nm. The extraction recovery of rimonabant in plasma at three quality control
(QC) samples was ranged from 84.17% to 92.64%. The calibration curve was linear for the concentration
range of 20–400 ng/ml with the correlation coefficient (r
2
) above 0.9921. The method was specific and
sensitive with the limit of quantification of 20 ng/ml. The accuracy and precision values obtained from
six different sets of QC samples analyzed in separate occasions ranged from 88.13% to 106.48% and 0.13%
to 3.61%, respectively. In stability tests, rimonabant in human plasma was stable during storage and assay
procedure. The method is very simple, sensitive and economical and the assay was applied to human
plasma samples in a clinical (pharmacokinetic) study of rimonabant. |
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