Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone

The potential of thymoquinone, an active component of Nigella Sativa, in lowering chemotherapeuticassociated toxicity of cyclophosphamide in the testis, epididymis and sperm DNA of adolescent male mice was investigated. Cyclophosphamide and thymoquinone were administered intraperitoneally by a sin...

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Main Authors:	Saheera, Kamarzaman, Sha'ban, Munirah, Abdul Rahman, Suzanah
Format:	Article
Language:	English
Published:	Center for Enhancing Knowledge (CEK) 2013
Subjects:	Q Science (General) R Medicine (General)
Online Access:	http://irep.iium.edu.my/32053/ http://irep.iium.edu.my/32053/ http://irep.iium.edu.my/32053/1/EIJST_-_Saheera_et._al.pdf

id	iium-32053
recordtype	eprints
spelling	iium-320532016-02-02T04:57:48Z http://irep.iium.edu.my/32053/ Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone Saheera, Kamarzaman Sha'ban, Munirah Abdul Rahman, Suzanah Q Science (General) R Medicine (General) The potential of thymoquinone, an active component of Nigella Sativa, in lowering chemotherapeuticassociated toxicity of cyclophosphamide in the testis, epididymis and sperm DNA of adolescent male mice was investigated. Cyclophosphamide and thymoquinone were administered intraperitoneally by a single injection at 200 mg/kg and 10 mg/kg on alternate days, respectively. Effects were observed on days 5, 32 and 53. Results show that thirty-two days following exposure to cyclophosphamide, the spermatogonia cells was reduced by 52% than that in the control group, p<0.05. Treatment with cyclophosphamide also induced significant toxicity as shown by hypertrophy in the mean diameter of seminiferous tubules and tubular lumen (p<0.05), vacuolization of spermatogonial layers and destruction of interstitial tissues. Administration of thymoquinone exhibited significant reduction in overall toxicity caused by cyclophosphamide. The integrity of the seminiferous tubule was well preserved and the numbers of total DNA damaged cells were reduced from 49% to 4% at 32 days. Center for Enhancing Knowledge (CEK) 2013-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32053/1/EIJST_-_Saheera_et._al.pdf Saheera, Kamarzaman and Sha'ban, Munirah and Abdul Rahman, Suzanah (2013) Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone. European International Journal of Science and Technology, 2 (7). pp. 119-136. ISSN 2304-9693 http://nu.kku.ac.th/eng/index.php/all-news/92-european-international-journal-of-science-and-technology
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) R Medicine (General)
spellingShingle	Q Science (General) R Medicine (General) Saheera, Kamarzaman Sha'ban, Munirah Abdul Rahman, Suzanah Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone
description	The potential of thymoquinone, an active component of Nigella Sativa, in lowering chemotherapeuticassociated toxicity of cyclophosphamide in the testis, epididymis and sperm DNA of adolescent male mice was investigated. Cyclophosphamide and thymoquinone were administered intraperitoneally by a single injection at 200 mg/kg and 10 mg/kg on alternate days, respectively. Effects were observed on days 5, 32 and 53. Results show that thirty-two days following exposure to cyclophosphamide, the spermatogonia cells was reduced by 52% than that in the control group, p<0.05. Treatment with cyclophosphamide also induced significant toxicity as shown by hypertrophy in the mean diameter of seminiferous tubules and tubular lumen (p<0.05), vacuolization of spermatogonial layers and destruction of interstitial tissues. Administration of thymoquinone exhibited significant reduction in overall toxicity caused by cyclophosphamide. The integrity of the seminiferous tubule was well preserved and the numbers of total DNA damaged cells were reduced from 49% to 4% at 32 days.
format	Article
author	Saheera, Kamarzaman Sha'ban, Munirah Abdul Rahman, Suzanah
author_facet	Saheera, Kamarzaman Sha'ban, Munirah Abdul Rahman, Suzanah
author_sort	Saheera, Kamarzaman
title	Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone
title_short	Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone
title_full	Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone
title_fullStr	Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone
title_full_unstemmed	Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone
title_sort	effects on mouse spermatogenesis and dna fragmentation following exposure to cyclophosphamide and thymoquinone
publisher	Center for Enhancing Knowledge (CEK)
publishDate	2013
url	http://irep.iium.edu.my/32053/ http://irep.iium.edu.my/32053/ http://irep.iium.edu.my/32053/1/EIJST_-_Saheera_et._al.pdf
first_indexed	2023-09-18T20:46:15Z
last_indexed	2023-09-18T20:46:15Z
_version_	1777409694361452544

Effects on mouse spermatogenesis and DNA fragmentation following exposure to cyclophosphamide and thymoquinone

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