Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay

Dinoflagellates belonging to the genus Prorocentrum were studied for toxicological and toxin production properties by cell assays and liquid chromatographic methods, using fluorescence and mass-spectrometry detection (LC-FD, LC-MS). Cytotoxicity characterised by IC50 and morphological changes was ex...

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Main Authors:	Caillaud, A., Canete, E., Mallat, E., Fernandez, M., Mohammad Noor, Normawaty, Moestrup, Ojvind, Franco, J.M.
Format:	Conference or Workshop Item
Language:	English
Published:	2006
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/32129/ http://irep.iium.edu.my/32129/1/Evaluation_of_the_toxicity_of_Prorocentrum_by_LCMS.pdf

id	iium-32129
recordtype	eprints
spelling	iium-321292016-05-12T00:19:50Z http://irep.iium.edu.my/32129/ Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay Caillaud, A. Canete, E. Mallat, E. Fernandez, M. Mohammad Noor, Normawaty Moestrup, Ojvind Franco, J.M. Q Science (General) Dinoflagellates belonging to the genus Prorocentrum were studied for toxicological and toxin production properties by cell assays and liquid chromatographic methods, using fluorescence and mass-spectrometry detection (LC-FD, LC-MS). Cytotoxicity characterised by IC50 and morphological changes was examined. IC50 ranged from 2.103 cell equivalents mL-1 for P. cf faustiae to 133.103 for P. rhathymum. Morphological changes such as rounding and membrane blebbing were observed in fibroblasts exposed to Prorocentrum belizeanum and all Prorocentrum lima from Malaysia suggesting the presence of okadaic acid (OA). Analytical measurements confirmed the production of OA. However toxicity not correlated with the presence of OA suggests the production of derivates of OA or others toxin. 2006-09 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/32129/1/Evaluation_of_the_toxicity_of_Prorocentrum_by_LCMS.pdf Caillaud, A. and Canete, E. and Mallat, E. and Fernandez, M. and Mohammad Noor, Normawaty and Moestrup, Ojvind and Franco, J.M. (2006) Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay. In: 12th International Conference on Harmful Algae, 4-8 September 2006, Copenhagen, Denmark.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Caillaud, A. Canete, E. Mallat, E. Fernandez, M. Mohammad Noor, Normawaty Moestrup, Ojvind Franco, J.M. Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay
description	Dinoflagellates belonging to the genus Prorocentrum were studied for toxicological and toxin production properties by cell assays and liquid chromatographic methods, using fluorescence and mass-spectrometry detection (LC-FD, LC-MS). Cytotoxicity characterised by IC50 and morphological changes was examined. IC50 ranged from 2.103 cell equivalents mL-1 for P. cf faustiae to 133.103 for P. rhathymum. Morphological changes such as rounding and membrane blebbing were observed in fibroblasts exposed to Prorocentrum belizeanum and all Prorocentrum lima from Malaysia suggesting the presence of okadaic acid (OA). Analytical measurements confirmed the production of OA. However toxicity not correlated with the presence of OA suggests the production of derivates of OA or others toxin.
format	Conference or Workshop Item
author	Caillaud, A. Canete, E. Mallat, E. Fernandez, M. Mohammad Noor, Normawaty Moestrup, Ojvind Franco, J.M.
author_facet	Caillaud, A. Canete, E. Mallat, E. Fernandez, M. Mohammad Noor, Normawaty Moestrup, Ojvind Franco, J.M.
author_sort	Caillaud, A.
title	Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay
title_short	Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay
title_full	Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay
title_fullStr	Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay
title_full_unstemmed	Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay
title_sort	evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay
publishDate	2006
url	http://irep.iium.edu.my/32129/ http://irep.iium.edu.my/32129/1/Evaluation_of_the_toxicity_of_Prorocentrum_by_LCMS.pdf
first_indexed	2023-09-18T20:46:22Z
last_indexed	2023-09-18T20:46:22Z
_version_	1777409701524275200

Evaluation of the toxicity of prorocentrum species by liquid chromatography mass-spectrometry and cell-based assay

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