Antioxidant, antimicrobial and total phenolic contents of Calophyllum symingtonianum
Objectives: To determine the total phenolic content, antioxidant and antimicrobial activities of the extracts from Calophyllum symingtonianum. Methods: The extracts were tested for their antioxidant activity by the DPPH radical scavenging assay and the β-carotene bleaching assay, while the anti...
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| Format: | Article |
| Language: | English |
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Coastal Life Medicine Media co., Limited
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/32204/ http://irep.iium.edu.my/32204/ http://irep.iium.edu.my/32204/1/Journal_of_coastal_medicine.pdf |
| Summary: | Objectives: To determine the total phenolic content, antioxidant and antimicrobial activities of the extracts from Calophyllum symingtonianum.
Methods: The extracts were tested for their antioxidant activity by the DPPH radical scavenging assay and the β-carotene bleaching assay, while the antimicrobial activity was determined by disc diffusion method.
Results: All the tested extracts showed antioxidant and antimicrobial properties. The extracts showed moderate antimicrobial activity against S. aureus with zone of inhibition values of 10, 11 and 12 mm for n-hexane, DCM and methanol extracts respectively at 30 µg/disc. n-Hexane showed low antimicrobial activity against P. aeruginosa (5 mm) at 30 µg/disc. The total phenolic test showed that methanol has high phenolic content (162.25 mg GAE/g of extract) compared to the other extracts.
Conclusion: The ability of the extracts to inhibit microbial growth at a concentration of 30 µg/disc indicated the its potent antimicrobial activity.
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