Evaluation of different extraction method for the isolation of cimetidine and ranitidine from human plasma for HPLC analysis

Cimetidine and ranitidine are classified as the H2-receptor antagonists (H2 antagonist). These classes of drugs used to block the action of histamine on parietal cells in the stomach, decreasing the production of acid by these cells. H2 antagonists are widely used in the treatment of gastro oesophag...

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Main Authors:	Helaluddin , Abul Bashar Mohammed, Awang, Mohamed, Rahman, Md. Mokhlesur, Khan, Umeed Ali
Format:	Conference or Workshop Item
Language:	English English
Published:	2012
Subjects:	QD Chemistry RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/32317/ http://irep.iium.edu.my/32317/1/PROCEEDING_COVER.pdf http://irep.iium.edu.my/32317/2/ID_197_-_pp_669-673_ABM_Helal_Uddin.pdf

id	iium-32317
recordtype	eprints
spelling	iium-323172017-09-05T04:40:50Z http://irep.iium.edu.my/32317/ Evaluation of different extraction method for the isolation of cimetidine and ranitidine from human plasma for HPLC analysis Helaluddin , Abul Bashar Mohammed Awang, Mohamed Rahman, Md. Mokhlesur Khan, Umeed Ali QD Chemistry RS Pharmacy and materia medica Cimetidine and ranitidine are classified as the H2-receptor antagonists (H2 antagonist). These classes of drugs used to block the action of histamine on parietal cells in the stomach, decreasing the production of acid by these cells. H2 antagonists are widely used in the treatment of gastro oesophageal reflux, gastric and duodenal ulceration. Malaysian government has implemented bio equivalence requirements for manufacturing all generic drugs, cimetidine and ranitidine are also listed with other high demand generic drugs. The study focused on the preliminary chromatographic detection and determination of cimetidine and ranitidine in human plasma which eventually can be used for bioequivalence study of generic formulation of cimetidine and ranitidine. Different sample extraction process were evaluated namely protein precipitation, solvent extraction and solid phase extraction (SPE). It was found for both cimetidine (89%) and ranitidine (87%) showed a better recovery using SPE. With solvent extraction cimetidine showed comparable recovery (85%) using ethyl acetate as a solvent of choice and dichloromethane for ranitidine (82%). For the protein precipitation technique both cimetidine (75%) and ranitidine (73%) showed a lower recovery compared to the other method. HPLC method was used for the analysis of the spiked plasma samples. Chromatographic separation was obtained using a C18 column and mobile phase was a combination of phosphate buffer and acetonitrile. Elution of cimetidine and ranitidine were detected with a UV-Vis detector at 230 nm wavelength. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/32317/1/PROCEEDING_COVER.pdf application/pdf en http://irep.iium.edu.my/32317/2/ID_197_-_pp_669-673_ABM_Helal_Uddin.pdf Helaluddin , Abul Bashar Mohammed and Awang, Mohamed and Rahman, Md. Mokhlesur and Khan, Umeed Ali (2012) Evaluation of different extraction method for the isolation of cimetidine and ranitidine from human plasma for HPLC analysis. In: Proceeding of International Conference on Science, Technology & Social Sciences, 20-22 Nov 2012, Kuantan, Pahang.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry RS Pharmacy and materia medica
spellingShingle	QD Chemistry RS Pharmacy and materia medica Helaluddin , Abul Bashar Mohammed Awang, Mohamed Rahman, Md. Mokhlesur Khan, Umeed Ali Evaluation of different extraction method for the isolation of cimetidine and ranitidine from human plasma for HPLC analysis
description	Cimetidine and ranitidine are classified as the H2-receptor antagonists (H2 antagonist). These classes of drugs used to block the action of histamine on parietal cells in the stomach, decreasing the production of acid by these cells. H2 antagonists are widely used in the treatment of gastro oesophageal reflux, gastric and duodenal ulceration. Malaysian government has implemented bio equivalence requirements for manufacturing all generic drugs, cimetidine and ranitidine are also listed with other high demand generic drugs. The study focused on the preliminary chromatographic detection and determination of cimetidine and ranitidine in human plasma which eventually can be used for bioequivalence study of generic formulation of cimetidine and ranitidine. Different sample extraction process were evaluated namely protein precipitation, solvent extraction and solid phase extraction (SPE). It was found for both cimetidine (89%) and ranitidine (87%) showed a better recovery using SPE. With solvent extraction cimetidine showed comparable recovery (85%) using ethyl acetate as a solvent of choice and dichloromethane for ranitidine (82%). For the protein precipitation technique both cimetidine (75%) and ranitidine (73%) showed a lower recovery compared to the other method. HPLC method was used for the analysis of the spiked plasma samples. Chromatographic separation was obtained using a C18 column and mobile phase was a combination of phosphate buffer and acetonitrile. Elution of cimetidine and ranitidine were detected with a UV-Vis detector at 230 nm wavelength.
format	Conference or Workshop Item
author	Helaluddin , Abul Bashar Mohammed Awang, Mohamed Rahman, Md. Mokhlesur Khan, Umeed Ali
author_facet	Helaluddin , Abul Bashar Mohammed Awang, Mohamed Rahman, Md. Mokhlesur Khan, Umeed Ali
author_sort	Helaluddin , Abul Bashar Mohammed
title	Evaluation of different extraction method for the isolation of cimetidine and ranitidine from human plasma for HPLC analysis
title_short	Evaluation of different extraction method for the isolation of cimetidine and ranitidine from human plasma for HPLC analysis
title_full	Evaluation of different extraction method for the isolation of cimetidine and ranitidine from human plasma for HPLC analysis
title_fullStr	Evaluation of different extraction method for the isolation of cimetidine and ranitidine from human plasma for HPLC analysis
title_full_unstemmed	Evaluation of different extraction method for the isolation of cimetidine and ranitidine from human plasma for HPLC analysis
title_sort	evaluation of different extraction method for the isolation of cimetidine and ranitidine from human plasma for hplc analysis
publishDate	2012
url	http://irep.iium.edu.my/32317/ http://irep.iium.edu.my/32317/1/PROCEEDING_COVER.pdf http://irep.iium.edu.my/32317/2/ID_197_-_pp_669-673_ABM_Helal_Uddin.pdf
first_indexed	2023-09-18T20:46:39Z
last_indexed	2023-09-18T20:46:39Z
_version_	1777409720131256320

Evaluation of different extraction method for the isolation of cimetidine and ranitidine from human plasma for HPLC analysis

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