Lignin modifying enzyme activities by some Malaysian white rot fungi

The possible biotechnological application of ligninolytic enzyme in a range of different fields has stimulated isolation and screening of potential microorganisms from local sources. Twelve white rot fungi (WRF) were isolated from around Gombak area (Malaysia) and sub cultured few times in order to...

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Main Authors:	Ruqayyah, Tijani I.D., Jamal, Parveen, Alam, Md. Zahangir, Mirghani, Mohamed Elwathig Saeed
Format:	Article
Language:	English
Published:	Africa City of Technology (ACT), 2011
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/3251/ http://irep.iium.edu.my/3251/ http://irep.iium.edu.my/3251/1/IndusMicrobiol-3.pdf

id	iium-3251
recordtype	eprints
spelling	iium-32512011-09-20T04:41:04Z http://irep.iium.edu.my/3251/ Lignin modifying enzyme activities by some Malaysian white rot fungi Ruqayyah, Tijani I.D. Jamal, Parveen Alam, Md. Zahangir Mirghani, Mohamed Elwathig Saeed TA164 Bioengineering The possible biotechnological application of ligninolytic enzyme in a range of different fields has stimulated isolation and screening of potential microorganisms from local sources. Twelve white rot fungi (WRF) were isolated from around Gombak area (Malaysia) and sub cultured few times in order to obtain pure cultures. These strains were first screened on solid media plates for their lignin modifying enzymes by using Poly R-478 and ABTS (2, 2’azinobis-(3-ethylbenzothiazoline- 6-sulfonic acid). Of the twelve white rot fungi tested seven were able to decolourize Poly R-478 and eleven showed significant ABTS-oxidation activities. The positive strains were then tested on liquid media for lignin peroxidases (LiP), manganese peroxidases (MnP) and laccase (Lac) activity. Six strains showed potential to secrete all the three enzymes. One strain was able to secrete only two enzymes and five strains secreted only one of the three enzymes. Africa City of Technology (ACT), 2011-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3251/1/IndusMicrobiol-3.pdf Ruqayyah, Tijani I.D. and Jamal, Parveen and Alam, Md. Zahangir and Mirghani, Mohamed Elwathig Saeed (2011) Lignin modifying enzyme activities by some Malaysian white rot fungi. ACT-Biotechnology Research Communications, 1 (1). pp. 14-19. ISSN 1858-5965 http://act.sd/arabic/act-brcs/index.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Ruqayyah, Tijani I.D. Jamal, Parveen Alam, Md. Zahangir Mirghani, Mohamed Elwathig Saeed Lignin modifying enzyme activities by some Malaysian white rot fungi
description	The possible biotechnological application of ligninolytic enzyme in a range of different fields has stimulated isolation and screening of potential microorganisms from local sources. Twelve white rot fungi (WRF) were isolated from around Gombak area (Malaysia) and sub cultured few times in order to obtain pure cultures. These strains were first screened on solid media plates for their lignin modifying enzymes by using Poly R-478 and ABTS (2, 2’azinobis-(3-ethylbenzothiazoline- 6-sulfonic acid). Of the twelve white rot fungi tested seven were able to decolourize Poly R-478 and eleven showed significant ABTS-oxidation activities. The positive strains were then tested on liquid media for lignin peroxidases (LiP), manganese peroxidases (MnP) and laccase (Lac) activity. Six strains showed potential to secrete all the three enzymes. One strain was able to secrete only two enzymes and five strains secreted only one of the three enzymes.
format	Article
author	Ruqayyah, Tijani I.D. Jamal, Parveen Alam, Md. Zahangir Mirghani, Mohamed Elwathig Saeed
author_facet	Ruqayyah, Tijani I.D. Jamal, Parveen Alam, Md. Zahangir Mirghani, Mohamed Elwathig Saeed
author_sort	Ruqayyah, Tijani I.D.
title	Lignin modifying enzyme activities by some Malaysian white rot fungi
title_short	Lignin modifying enzyme activities by some Malaysian white rot fungi
title_full	Lignin modifying enzyme activities by some Malaysian white rot fungi
title_fullStr	Lignin modifying enzyme activities by some Malaysian white rot fungi
title_full_unstemmed	Lignin modifying enzyme activities by some Malaysian white rot fungi
title_sort	lignin modifying enzyme activities by some malaysian white rot fungi
publisher	Africa City of Technology (ACT),
publishDate	2011
url	http://irep.iium.edu.my/3251/ http://irep.iium.edu.my/3251/ http://irep.iium.edu.my/3251/1/IndusMicrobiol-3.pdf
first_indexed	2023-09-18T20:11:00Z
last_indexed	2023-09-18T20:11:00Z
_version_	1777407477240823808

Lignin modifying enzyme activities by some Malaysian white rot fungi

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