Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina
Emerging antibiotic resistance among the pathogenic microorganism has triggered the discovery of various antimicrobial compounds from marine source. Secondary metabolites from plants especially mangroves is traditionally being used widely as antimicrobials. Hence, present study was aimed to determ...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
IDOSI Publication
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/32668/ http://irep.iium.edu.my/32668/ http://irep.iium.edu.my/32668/ http://irep.iium.edu.my/32668/1/paper_5.pdf |
| Summary: | Emerging antibiotic resistance among the pathogenic microorganism has triggered the discovery of
various antimicrobial compounds from marine source. Secondary metabolites from plants especially mangroves
is traditionally being used widely as antimicrobials. Hence, present study was aimed to determine the
antibacterial and cytotoxic properties of the selected mangrove plant leaves (Bruguiera cyclindrica, Sonneratia
caseolaris, Luminitzera racemosa, Rhizophora apiculata, Avicennia alba, Acrostichum aureum, Nypa
fruticans, Pandanus odoratissimus, Hibiscus tiliaceous and Derris trifoliate) against aquaculture pathogenic
strains of genus. Methanol and aqueous extracts of leaves samples were prepared. The antibacterial activity
was determined by using disc diffusion method against six pathogenic Gram-negative Vibrio species, such as
Vibrio alginolyticus and V. parahaemolyticus (isolated from Scylla serrata, mangrove crab), V. alginolyticus
and V. parahaemolyticus (from Giant prawn), V. alginolyticus (from Tiger prawn) and also V. parahaemolyticus
(from Reference strain ATCC 17802). Methanolic extracts of S. caseolaris produced significant inhibition zone
against all the pathogenic Vibrio spp. except for V. parahaemolyticus (from Giant prawn). Minimum inhibitory
concentration test (MIC) and cytotoxicity (LC50) test using Artemia salina as a test animal in sterile ELISA
(Enzyme-Linked Immunosorbent Assay) microplates showed the significant antimicrobial properties of
methanolic extract of S. caesolaris. Overall, present study proved the active antimicrobial compounds in
methanolic extract of S. caesolaris which could be used against Vibrio pathogens in aquaculture. |
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