Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina

Emerging antibiotic resistance among the pathogenic microorganism has triggered the discovery of various antimicrobial compounds from marine source. Secondary metabolites from plants especially mangroves is traditionally being used widely as antimicrobials. Hence, present study was aimed to determ...

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Main Authors:	Ahmad, Ahmad Shamsuddin, Musa, Najiah, Assaw, Suvik, Mohtar, Noor Azariyah, Yunus, Kamaruzzaman, Abd. Wahid, Mohd. Effendy, John, Akbar
Format:	Article
Language:	English
Published:	IDOSI Publication 2013
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/32668/ http://irep.iium.edu.my/32668/ http://irep.iium.edu.my/32668/ http://irep.iium.edu.my/32668/1/paper_5.pdf

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spelling	iium-326682013-11-12T04:42:36Z http://irep.iium.edu.my/32668/ Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina Ahmad, Ahmad Shamsuddin Musa, Najiah Assaw, Suvik Mohtar, Noor Azariyah Yunus, Kamaruzzaman Abd. Wahid, Mohd. Effendy John, Akbar Q Science (General) Emerging antibiotic resistance among the pathogenic microorganism has triggered the discovery of various antimicrobial compounds from marine source. Secondary metabolites from plants especially mangroves is traditionally being used widely as antimicrobials. Hence, present study was aimed to determine the antibacterial and cytotoxic properties of the selected mangrove plant leaves (Bruguiera cyclindrica, Sonneratia caseolaris, Luminitzera racemosa, Rhizophora apiculata, Avicennia alba, Acrostichum aureum, Nypa fruticans, Pandanus odoratissimus, Hibiscus tiliaceous and Derris trifoliate) against aquaculture pathogenic strains of genus. Methanol and aqueous extracts of leaves samples were prepared. The antibacterial activity was determined by using disc diffusion method against six pathogenic Gram-negative Vibrio species, such as Vibrio alginolyticus and V. parahaemolyticus (isolated from Scylla serrata, mangrove crab), V. alginolyticus and V. parahaemolyticus (from Giant prawn), V. alginolyticus (from Tiger prawn) and also V. parahaemolyticus (from Reference strain ATCC 17802). Methanolic extracts of S. caseolaris produced significant inhibition zone against all the pathogenic Vibrio spp. except for V. parahaemolyticus (from Giant prawn). Minimum inhibitory concentration test (MIC) and cytotoxicity (LC50) test using Artemia salina as a test animal in sterile ELISA (Enzyme-Linked Immunosorbent Assay) microplates showed the significant antimicrobial properties of methanolic extract of S. caesolaris. Overall, present study proved the active antimicrobial compounds in methanolic extract of S. caesolaris which could be used against Vibrio pathogens in aquaculture. IDOSI Publication 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32668/1/paper_5.pdf Ahmad, Ahmad Shamsuddin and Musa, Najiah and Assaw, Suvik and Mohtar, Noor Azariyah and Yunus, Kamaruzzaman and Abd. Wahid, Mohd. Effendy and John, Akbar (2013) Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina. World Applied Sciences Journal , 25 (2). pp. 333-340. ISSN 1818-4952 http://www.idosi.org/wasj/wasj25%282%2913/22.pdf 10.5829/idosi.wasj.2013.25.02.688
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Ahmad, Ahmad Shamsuddin Musa, Najiah Assaw, Suvik Mohtar, Noor Azariyah Yunus, Kamaruzzaman Abd. Wahid, Mohd. Effendy John, Akbar Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina
description	Emerging antibiotic resistance among the pathogenic microorganism has triggered the discovery of various antimicrobial compounds from marine source. Secondary metabolites from plants especially mangroves is traditionally being used widely as antimicrobials. Hence, present study was aimed to determine the antibacterial and cytotoxic properties of the selected mangrove plant leaves (Bruguiera cyclindrica, Sonneratia caseolaris, Luminitzera racemosa, Rhizophora apiculata, Avicennia alba, Acrostichum aureum, Nypa fruticans, Pandanus odoratissimus, Hibiscus tiliaceous and Derris trifoliate) against aquaculture pathogenic strains of genus. Methanol and aqueous extracts of leaves samples were prepared. The antibacterial activity was determined by using disc diffusion method against six pathogenic Gram-negative Vibrio species, such as Vibrio alginolyticus and V. parahaemolyticus (isolated from Scylla serrata, mangrove crab), V. alginolyticus and V. parahaemolyticus (from Giant prawn), V. alginolyticus (from Tiger prawn) and also V. parahaemolyticus (from Reference strain ATCC 17802). Methanolic extracts of S. caseolaris produced significant inhibition zone against all the pathogenic Vibrio spp. except for V. parahaemolyticus (from Giant prawn). Minimum inhibitory concentration test (MIC) and cytotoxicity (LC50) test using Artemia salina as a test animal in sterile ELISA (Enzyme-Linked Immunosorbent Assay) microplates showed the significant antimicrobial properties of methanolic extract of S. caesolaris. Overall, present study proved the active antimicrobial compounds in methanolic extract of S. caesolaris which could be used against Vibrio pathogens in aquaculture.
format	Article
author	Ahmad, Ahmad Shamsuddin Musa, Najiah Assaw, Suvik Mohtar, Noor Azariyah Yunus, Kamaruzzaman Abd. Wahid, Mohd. Effendy John, Akbar
author_facet	Ahmad, Ahmad Shamsuddin Musa, Najiah Assaw, Suvik Mohtar, Noor Azariyah Yunus, Kamaruzzaman Abd. Wahid, Mohd. Effendy John, Akbar
author_sort	Ahmad, Ahmad Shamsuddin
title	Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina
title_short	Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina
title_full	Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina
title_fullStr	Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina
title_full_unstemmed	Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina
title_sort	antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina
publisher	IDOSI Publication
publishDate	2013
url	http://irep.iium.edu.my/32668/ http://irep.iium.edu.my/32668/ http://irep.iium.edu.my/32668/ http://irep.iium.edu.my/32668/1/paper_5.pdf
first_indexed	2023-09-18T20:47:10Z
last_indexed	2023-09-18T20:47:10Z
_version_	1777409752346656768

Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina

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