Microbial contamination of equipment in the dental laboratory

Objectives: To determine the extent of microbial contamination on the high touch areas of equipment in the dental laboratory. Method: A dental laboratory based cross-sectional study was carried out on four selected high touch areas, which were the vibrator (power switch), the cast trimmer (power sw...

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Main Authors:	Cheong, Joo Ming, Hussin, Akbar Sham, Ichwan, Solachuddin J. A., Khalid, Rafiq, Abidin, Sulhi
Format:	Conference or Workshop Item
Language:	English
Published:	2013
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/32825/ http://irep.iium.edu.my/32825/1/IADR_Abstract.pdf

id	iium-32825
recordtype	eprints
spelling	iium-328252013-11-25T06:34:18Z http://irep.iium.edu.my/32825/ Microbial contamination of equipment in the dental laboratory Cheong, Joo Ming Hussin, Akbar Sham Ichwan, Solachuddin J. A. Khalid, Rafiq Abidin, Sulhi RK Dentistry Objectives: To determine the extent of microbial contamination on the high touch areas of equipment in the dental laboratory. Method: A dental laboratory based cross-sectional study was carried out on four selected high touch areas, which were the vibrator (power switch), the cast trimmer (power switch) and the denture polisher (power switch & speed control knob). Sterile cotton swabs were used to swab these four areas, smeared onto nutrient agar media and then incubated for 72 hours. The colony forming units (CFU) were counted after 72 hours to determine the extent of microbial contamination. Sample collection was carried out for 3 consecutive days. As a control, the same areas were disinfected and samples were taken exactly as before. Investigation of similar procedure was carried out for another week. A total of 48 sample plates were collected, 24 before disinfection and another 24 after disinfection. Results: A before and after comparison of mean CFU count in the four high touch areas showed both speed control knob (216/54.33) and power switch (189.67/9.83) of the denture polishing machine had the highest CFU count, followed by the cast trimmer (68.17/18) and finally the vibrator (50/7). Before disinfection, the minimum, maximum and mean ± SD bacteria count in 24 plates were 19, 410 and 131.08 ± 105.77 respectively. The minimum, maximum and mean ± (SD) bacteria count in 24 plates after disinfection were 1, 102 and 21.96 ± 28.303 respectively. Reduction in mean of CFU count after surface disinfection using paired T-test was statistically significant (p<0.05). Conclusion: There is high level of bacterial contamination of the high touch areas in the absence of surface disinfection. Therefore, disinfection procedures as are conducted in dental clinic should be implemented in the dental laboratory. Keywords: Dental laboratory, High touch areas, Bacterial contamination, Disinfection 2013-08-21 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/32825/1/IADR_Abstract.pdf Cheong, Joo Ming and Hussin, Akbar Sham and Ichwan, Solachuddin J. A. and Khalid, Rafiq and Abidin, Sulhi (2013) Microbial contamination of equipment in the dental laboratory. In: 2nd Meeting of the International Association for Dental Research- Asia Pacific Region, 21-23 Aug 2013, Plaza Athenee, Bangkok, Thailand.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Cheong, Joo Ming Hussin, Akbar Sham Ichwan, Solachuddin J. A. Khalid, Rafiq Abidin, Sulhi Microbial contamination of equipment in the dental laboratory
description	Objectives: To determine the extent of microbial contamination on the high touch areas of equipment in the dental laboratory. Method: A dental laboratory based cross-sectional study was carried out on four selected high touch areas, which were the vibrator (power switch), the cast trimmer (power switch) and the denture polisher (power switch & speed control knob). Sterile cotton swabs were used to swab these four areas, smeared onto nutrient agar media and then incubated for 72 hours. The colony forming units (CFU) were counted after 72 hours to determine the extent of microbial contamination. Sample collection was carried out for 3 consecutive days. As a control, the same areas were disinfected and samples were taken exactly as before. Investigation of similar procedure was carried out for another week. A total of 48 sample plates were collected, 24 before disinfection and another 24 after disinfection. Results: A before and after comparison of mean CFU count in the four high touch areas showed both speed control knob (216/54.33) and power switch (189.67/9.83) of the denture polishing machine had the highest CFU count, followed by the cast trimmer (68.17/18) and finally the vibrator (50/7). Before disinfection, the minimum, maximum and mean ± SD bacteria count in 24 plates were 19, 410 and 131.08 ± 105.77 respectively. The minimum, maximum and mean ± (SD) bacteria count in 24 plates after disinfection were 1, 102 and 21.96 ± 28.303 respectively. Reduction in mean of CFU count after surface disinfection using paired T-test was statistically significant (p<0.05). Conclusion: There is high level of bacterial contamination of the high touch areas in the absence of surface disinfection. Therefore, disinfection procedures as are conducted in dental clinic should be implemented in the dental laboratory. Keywords: Dental laboratory, High touch areas, Bacterial contamination, Disinfection
format	Conference or Workshop Item
author	Cheong, Joo Ming Hussin, Akbar Sham Ichwan, Solachuddin J. A. Khalid, Rafiq Abidin, Sulhi
author_facet	Cheong, Joo Ming Hussin, Akbar Sham Ichwan, Solachuddin J. A. Khalid, Rafiq Abidin, Sulhi
author_sort	Cheong, Joo Ming
title	Microbial contamination of equipment in the dental laboratory
title_short	Microbial contamination of equipment in the dental laboratory
title_full	Microbial contamination of equipment in the dental laboratory
title_fullStr	Microbial contamination of equipment in the dental laboratory
title_full_unstemmed	Microbial contamination of equipment in the dental laboratory
title_sort	microbial contamination of equipment in the dental laboratory
publishDate	2013
url	http://irep.iium.edu.my/32825/ http://irep.iium.edu.my/32825/1/IADR_Abstract.pdf
first_indexed	2023-09-18T20:47:22Z
last_indexed	2023-09-18T20:47:22Z
_version_	1777409765252530176

Microbial contamination of equipment in the dental laboratory

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