Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats

Background Melastoma malabathricum L. (Melastomaceae) is a small shrub with various medicinal uses. The present study was carried out to determine the hepatoprotective activity of methanol extract of M. malabathricum leaves (MEMM) against the paracetamol-induced liver toxicity in rats model. Metho...

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Main Authors:	Mamat, Siti Syariah, Kamarolzaman, Mohammad Fauzi Fahmi, Yahya, Farhana, Mahmood, Nur Diyana, Shahril, Mohamad Syahmi, Jakius, Krystal Fredolin, Mohtaruddin, Norhafizah, Darnis, Deny Susanti, Bakhtiar, M. Taher, Zakaria, Zainul Amiruddin
Format:	Article
Language:	English
Published:	BioMed Central 2013
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/33006/ http://irep.iium.edu.my/33006/ http://irep.iium.edu.my/33006/ http://irep.iium.edu.my/33006/1/BMC_Health_Melastoma.pdf

id	iium-33006
recordtype	eprints
spelling	iium-330062014-12-08T09:38:18Z http://irep.iium.edu.my/33006/ Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Yahya, Farhana Mahmood, Nur Diyana Shahril, Mohamad Syahmi Jakius, Krystal Fredolin Mohtaruddin, Norhafizah Darnis, Deny Susanti Bakhtiar, M. Taher Zakaria, Zainul Amiruddin RS Pharmacy and materia medica Background Melastoma malabathricum L. (Melastomaceae) is a small shrub with various medicinal uses. The present study was carried out to determine the hepatoprotective activity of methanol extract of M. malabathricum leaves (MEMM) against the paracetamol-induced liver toxicity in rats model. Methods The respective chemicals and herbal solutions (10% DMSO, 200 mg/kg silymarin or MEMM (50, 250 and 500 mg/kg)) were administered orally to rats once everyday for 7 days followed by the hepatotoxicity assay. The blood samples and livers were collected and subjected to biochemical and microscopical analysis. Prior to the hepatoprotective study, MEMM was subjected to determination of the total phenolic content (TPC) and the antioxidant properties using several standard assays (e.g. 2, 2-diphenyl-1-picrylhydrazyl- and superoxide anion- radical scavenging assay, and oxygen radical absorbance capacity assay). Results MEMM exerted significant (p < 0.05) and high antioxidant activity in which high TPC was recorded; while in the hepatotoxicity study, the extract exhibited significant hepatoprotective effects against the paracetamol-induced hepatotoxic model. The results observed for serum liver enzymes (ALT, ALP and AST) as well as the microscopic observations and microscopic scoring supported the hepatoprotective potential of MEMM. The phytochemical and HPLC analysis of MEMM demonstrated the presence of flavonoids as its major constituents. Conclusions The MEMM-induced hepatoprotective activity could be allied partly to its antioxidant activity and the presence of flavonoids. BioMed Central 2013-11-23 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33006/1/BMC_Health_Melastoma.pdf Mamat, Siti Syariah and Kamarolzaman, Mohammad Fauzi Fahmi and Yahya, Farhana and Mahmood, Nur Diyana and Shahril, Mohamad Syahmi and Jakius, Krystal Fredolin and Mohtaruddin, Norhafizah and Darnis, Deny Susanti and Bakhtiar, M. Taher and Zakaria, Zainul Amiruddin (2013) Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats. BMC Complementary and Alternative Medicine, 13. p. 326. ISSN 1472-6882 http://www.biomedcentral.com/1472-6882/13/326/abstract 10.1186/1472-6882-13-326
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Yahya, Farhana Mahmood, Nur Diyana Shahril, Mohamad Syahmi Jakius, Krystal Fredolin Mohtaruddin, Norhafizah Darnis, Deny Susanti Bakhtiar, M. Taher Zakaria, Zainul Amiruddin Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
description	Background Melastoma malabathricum L. (Melastomaceae) is a small shrub with various medicinal uses. The present study was carried out to determine the hepatoprotective activity of methanol extract of M. malabathricum leaves (MEMM) against the paracetamol-induced liver toxicity in rats model. Methods The respective chemicals and herbal solutions (10% DMSO, 200 mg/kg silymarin or MEMM (50, 250 and 500 mg/kg)) were administered orally to rats once everyday for 7 days followed by the hepatotoxicity assay. The blood samples and livers were collected and subjected to biochemical and microscopical analysis. Prior to the hepatoprotective study, MEMM was subjected to determination of the total phenolic content (TPC) and the antioxidant properties using several standard assays (e.g. 2, 2-diphenyl-1-picrylhydrazyl- and superoxide anion- radical scavenging assay, and oxygen radical absorbance capacity assay). Results MEMM exerted significant (p < 0.05) and high antioxidant activity in which high TPC was recorded; while in the hepatotoxicity study, the extract exhibited significant hepatoprotective effects against the paracetamol-induced hepatotoxic model. The results observed for serum liver enzymes (ALT, ALP and AST) as well as the microscopic observations and microscopic scoring supported the hepatoprotective potential of MEMM. The phytochemical and HPLC analysis of MEMM demonstrated the presence of flavonoids as its major constituents. Conclusions The MEMM-induced hepatoprotective activity could be allied partly to its antioxidant activity and the presence of flavonoids.
format	Article
author	Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Yahya, Farhana Mahmood, Nur Diyana Shahril, Mohamad Syahmi Jakius, Krystal Fredolin Mohtaruddin, Norhafizah Darnis, Deny Susanti Bakhtiar, M. Taher Zakaria, Zainul Amiruddin
author_facet	Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Yahya, Farhana Mahmood, Nur Diyana Shahril, Mohamad Syahmi Jakius, Krystal Fredolin Mohtaruddin, Norhafizah Darnis, Deny Susanti Bakhtiar, M. Taher Zakaria, Zainul Amiruddin
author_sort	Mamat, Siti Syariah
title	Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
title_short	Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
title_full	Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
title_fullStr	Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
title_full_unstemmed	Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
title_sort	methanol extract of melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
publisher	BioMed Central
publishDate	2013
url	http://irep.iium.edu.my/33006/ http://irep.iium.edu.my/33006/ http://irep.iium.edu.my/33006/ http://irep.iium.edu.my/33006/1/BMC_Health_Melastoma.pdf
first_indexed	2023-09-18T20:47:40Z
last_indexed	2023-09-18T20:47:40Z
_version_	1777409783582687232

Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats

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