1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus

1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus

medicinal herb to treat several maladies. Proton nuclear magnetic resonance (1H-NMR) combined with principal component analysis (PCA) and partial least-squares analysis (PLS) was applied to distinguish variations among C. caudatus materials processed with various drying techniques. A discriminator...

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Main Authors: Mediani, Ahmed, Abas, Faridah, Khatib, Alfi, Maulidiani, H, Shaari, Khozirah, Choi, Young Hae, Lajis, Nordin
Format: Article
Language:English
Published: Elsevier 2012
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/33293/
http://irep.iium.edu.my/33293/
http://irep.iium.edu.my/33293/
http://irep.iium.edu.my/33293/2/Mediani_et_al._2012.pdf
id iium-33293
recordtype eprints
spelling iium-332932013-12-13T08:48:39Z http://irep.iium.edu.my/33293/ 1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus Mediani, Ahmed Abas, Faridah Khatib, Alfi Maulidiani, H Shaari, Khozirah Choi, Young Hae Lajis, Nordin QD Chemistry medicinal herb to treat several maladies. Proton nuclear magnetic resonance (1H-NMR) combined with principal component analysis (PCA) and partial least-squares analysis (PLS) was applied to distinguish variations among C. caudatus materials processed with various drying techniques. A discriminatory report on the metabolites responsible for the variation between air (AD), oven (OD) and freeze (FD) dryings of C. caudatus samples was successfully achieved using 1H-NMR-based metabolomics. The correlation between antioxidant activity (AA) represented by radical DPPH scavenging activity and metabolite variation among processed samples dried in three different ways was also determined by partial least-squares analysis (PLS). The PCA and PLS scores showed a noticeable and clear separation into three clusters representing the three drying methods by PC1 and PC2, with an eigenvalue of 77.9%. Various assigned 1H-NMR chemical shifts referring to themetabolites responsible for sample variation were ascribed. The identified compounds were β- and α-glucose, chlorogenic acid, catechin, rutin, quercetin, quercetin 3-O-rhamnoside, quercetin 3-O-β-arabinofuranoside and quercetin 3-O-β-glucoside. This research demonstrates thatmost of the identified compounds were present in all samples from the three processing methods. However, there was marked variance in the concentration of the constituents present. Elsevier 2012-09-14 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33293/2/Mediani_et_al._2012.pdf Mediani, Ahmed and Abas, Faridah and Khatib, Alfi and Maulidiani, H and Shaari, Khozirah and Choi, Young Hae and Lajis, Nordin (2012) 1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus. Food Research International, 49 (2). pp. 763-770. ISSN 0963-9969 http://www.sciencedirect.com/science/article/pii/S096399691200381X 10.1016/j.foodres.2012.09.022
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Mediani, Ahmed
Abas, Faridah
Khatib, Alfi
Maulidiani, H
Shaari, Khozirah
Choi, Young Hae
Lajis, Nordin
1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus
description medicinal herb to treat several maladies. Proton nuclear magnetic resonance (1H-NMR) combined with principal component analysis (PCA) and partial least-squares analysis (PLS) was applied to distinguish variations among C. caudatus materials processed with various drying techniques. A discriminatory report on the metabolites responsible for the variation between air (AD), oven (OD) and freeze (FD) dryings of C. caudatus samples was successfully achieved using 1H-NMR-based metabolomics. The correlation between antioxidant activity (AA) represented by radical DPPH scavenging activity and metabolite variation among processed samples dried in three different ways was also determined by partial least-squares analysis (PLS). The PCA and PLS scores showed a noticeable and clear separation into three clusters representing the three drying methods by PC1 and PC2, with an eigenvalue of 77.9%. Various assigned 1H-NMR chemical shifts referring to themetabolites responsible for sample variation were ascribed. The identified compounds were β- and α-glucose, chlorogenic acid, catechin, rutin, quercetin, quercetin 3-O-rhamnoside, quercetin 3-O-β-arabinofuranoside and quercetin 3-O-β-glucoside. This research demonstrates thatmost of the identified compounds were present in all samples from the three processing methods. However, there was marked variance in the concentration of the constituents present.
format Article
author Mediani, Ahmed
Abas, Faridah
Khatib, Alfi
Maulidiani, H
Shaari, Khozirah
Choi, Young Hae
Lajis, Nordin
author_facet Mediani, Ahmed
Abas, Faridah
Khatib, Alfi
Maulidiani, H
Shaari, Khozirah
Choi, Young Hae
Lajis, Nordin
author_sort Mediani, Ahmed
title 1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus
title_short 1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus
title_full 1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus
title_fullStr 1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus
title_full_unstemmed 1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus
title_sort 1h-nmr-based metabolomics approach to understanding the drying effects on the phytochemicals in cosmos caudatus
publisher Elsevier
publishDate 2012
url http://irep.iium.edu.my/33293/
http://irep.iium.edu.my/33293/
http://irep.iium.edu.my/33293/
http://irep.iium.edu.my/33293/2/Mediani_et_al._2012.pdf
first_indexed 2023-09-18T20:48:07Z
last_indexed 2023-09-18T20:48:07Z
_version_ 1777409811976028160

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