Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis
The ‘Ulam’, a traditional Malay dish, are plants that can be eaten raw, as a form of local salad. The shoots and young leaves of Melicope ptelefolia are among the popular species, believed to be high in nutritional and medicinal values. The metabolomic fingerprinting analysis of the ethanolic extr...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/33305/ http://irep.iium.edu.my/33305/ http://irep.iium.edu.my/33305/ http://irep.iium.edu.my/33305/1/Shuib_et_al%2C_2011.pdf |
| Summary: | The ‘Ulam’, a traditional Malay dish, are plants that can be eaten raw, as a form of local salad. The shoots
and young leaves of Melicope ptelefolia are among the popular species, believed to be high in nutritional
and medicinal values. The metabolomic fingerprinting analysis of the ethanolic extracts of leaves of
M. ptelefolia was carried out using 1H Nuclear Magnetic Resonance (NMR) spectroscopy and multivariate
data analysis in order to differentiate young and mature leaves and to evaluate the variation of their
chemical composition. Principle component analysis (PCA) of the 1H NMR spectra showed a clear discrimination
between the young and mature leaves extracts by PC3 and PC4. The compounds responsible
for the differentiation were identified by comparison of 1H NMR chemical shifts and qualitative HPLC. The
young leaves were found to be richer in fatty acids and the levels of the three marker compounds, p-Ogeranylcoumaric
acid, 2,4,6-trihydroxy-3-geranylacetophenone and 2,4,6-trihydroxy-3-prenylacetophenone,
were clearly higher. The mature leaves contain higher levels of sugars and glycosidic components. |
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