Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis

Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis

The ‘Ulam’, a traditional Malay dish, are plants that can be eaten raw, as a form of local salad. The shoots and young leaves of Melicope ptelefolia are among the popular species, believed to be high in nutritional and medicinal values. The metabolomic fingerprinting analysis of the ethanolic extr...

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Main Authors: Shuib, Nor Hassifi, Shaari, Khozirah, Khatib, Alfi, Maulidiani, H, Kneer, Ralf, Zareen, Seema, Mohd. Raof, Salahudin, Lajis, Nordin, Neto, Victor
Format: Article
Language:English
Published: Elsevier 2011
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/33305/
http://irep.iium.edu.my/33305/
http://irep.iium.edu.my/33305/
http://irep.iium.edu.my/33305/1/Shuib_et_al%2C_2011.pdf
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recordtype eprints
spelling iium-333052013-12-13T03:10:25Z http://irep.iium.edu.my/33305/ Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis Shuib, Nor Hassifi Shaari, Khozirah Khatib, Alfi Maulidiani, H Kneer, Ralf Zareen, Seema Mohd. Raof, Salahudin Lajis, Nordin Neto, Victor QD Chemistry The ‘Ulam’, a traditional Malay dish, are plants that can be eaten raw, as a form of local salad. The shoots and young leaves of Melicope ptelefolia are among the popular species, believed to be high in nutritional and medicinal values. The metabolomic fingerprinting analysis of the ethanolic extracts of leaves of M. ptelefolia was carried out using 1H Nuclear Magnetic Resonance (NMR) spectroscopy and multivariate data analysis in order to differentiate young and mature leaves and to evaluate the variation of their chemical composition. Principle component analysis (PCA) of the 1H NMR spectra showed a clear discrimination between the young and mature leaves extracts by PC3 and PC4. The compounds responsible for the differentiation were identified by comparison of 1H NMR chemical shifts and qualitative HPLC. The young leaves were found to be richer in fatty acids and the levels of the three marker compounds, p-Ogeranylcoumaric acid, 2,4,6-trihydroxy-3-geranylacetophenone and 2,4,6-trihydroxy-3-prenylacetophenone, were clearly higher. The mature leaves contain higher levels of sugars and glycosidic components. Elsevier 2011-01-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33305/1/Shuib_et_al%2C_2011.pdf Shuib, Nor Hassifi and Shaari, Khozirah and Khatib, Alfi and Maulidiani, H and Kneer, Ralf and Zareen, Seema and Mohd. Raof, Salahudin and Lajis, Nordin and Neto, Victor (2011) Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis. Food Chemistry, 126 (2). pp. 640-645. ISSN 0308-8146 http://www.sciencedirect.com/science/journal/03088146 10.1016/j.foodchem.2010.10.043
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Shuib, Nor Hassifi
Shaari, Khozirah
Khatib, Alfi
Maulidiani, H
Kneer, Ralf
Zareen, Seema
Mohd. Raof, Salahudin
Lajis, Nordin
Neto, Victor
Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis
description The ‘Ulam’, a traditional Malay dish, are plants that can be eaten raw, as a form of local salad. The shoots and young leaves of Melicope ptelefolia are among the popular species, believed to be high in nutritional and medicinal values. The metabolomic fingerprinting analysis of the ethanolic extracts of leaves of M. ptelefolia was carried out using 1H Nuclear Magnetic Resonance (NMR) spectroscopy and multivariate data analysis in order to differentiate young and mature leaves and to evaluate the variation of their chemical composition. Principle component analysis (PCA) of the 1H NMR spectra showed a clear discrimination between the young and mature leaves extracts by PC3 and PC4. The compounds responsible for the differentiation were identified by comparison of 1H NMR chemical shifts and qualitative HPLC. The young leaves were found to be richer in fatty acids and the levels of the three marker compounds, p-Ogeranylcoumaric acid, 2,4,6-trihydroxy-3-geranylacetophenone and 2,4,6-trihydroxy-3-prenylacetophenone, were clearly higher. The mature leaves contain higher levels of sugars and glycosidic components.
format Article
author Shuib, Nor Hassifi
Shaari, Khozirah
Khatib, Alfi
Maulidiani, H
Kneer, Ralf
Zareen, Seema
Mohd. Raof, Salahudin
Lajis, Nordin
Neto, Victor
author_facet Shuib, Nor Hassifi
Shaari, Khozirah
Khatib, Alfi
Maulidiani, H
Kneer, Ralf
Zareen, Seema
Mohd. Raof, Salahudin
Lajis, Nordin
Neto, Victor
author_sort Shuib, Nor Hassifi
title Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis
title_short Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis
title_full Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis
title_fullStr Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis
title_full_unstemmed Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis
title_sort discrimination of young and mature leaves of melicope ptelefolia using 1h nmr and multivariate data analysis
publisher Elsevier
publishDate 2011
url http://irep.iium.edu.my/33305/
http://irep.iium.edu.my/33305/
http://irep.iium.edu.my/33305/
http://irep.iium.edu.my/33305/1/Shuib_et_al%2C_2011.pdf
first_indexed 2023-09-18T20:48:08Z
last_indexed 2023-09-18T20:48:08Z
_version_ 1777409813451374592

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