Development of new media formulation for the improvement of recombinant protein expression in E.Coli

The optimum growth media for the recombinant protein expression has the added advantage of improved cost effectiveness, enhanced down-stream processing, reduced wastewater, lower production costs and reduced investment in equipment. In this study a series of statistical optimization involves screeni...

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Bibliographic Details
Main Authors:	Mohd. Salleh, Hamzah, Hashim, Yumi Zuhanis Has-Yun, Nuge, Tamrin
Format:	Monograph
Language:	English
Published:	s.n 2012
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/33684/ http://irep.iium.edu.my/33684/ http://irep.iium.edu.my/33684/1/EDW_A11-159-950.pdf

id	iium-33684
recordtype	eprints
spelling	iium-336842015-05-13T06:35:54Z http://irep.iium.edu.my/33684/ Development of new media formulation for the improvement of recombinant protein expression in E.Coli Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun Nuge, Tamrin TP248.13 Biotechnology The optimum growth media for the recombinant protein expression has the added advantage of improved cost effectiveness, enhanced down-stream processing, reduced wastewater, lower production costs and reduced investment in equipment. In this study a series of statistical optimization involves screening, classical optimization (OFAT) and response surface methodology (RSM) is employed to develop an optimum media for bacteria growth. E. coli DH5 harboring phytase gene from Mycobacterium smegmatis is used as a model to develop the modified growth media. At the end of the study, the effectiveness of the newly developed growth media was also tested on few recombinant proteins. s.n 2012-02-17 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/33684/1/EDW_A11-159-950.pdf Mohd. Salleh, Hamzah and Hashim, Yumi Zuhanis Has-Yun and Nuge, Tamrin (2012) Development of new media formulation for the improvement of recombinant protein expression in E.Coli. Research Report. s.n, Kuala Lumpur. (Unpublished) EDW A11-159-0950
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun Nuge, Tamrin Development of new media formulation for the improvement of recombinant protein expression in E.Coli
description	The optimum growth media for the recombinant protein expression has the added advantage of improved cost effectiveness, enhanced down-stream processing, reduced wastewater, lower production costs and reduced investment in equipment. In this study a series of statistical optimization involves screening, classical optimization (OFAT) and response surface methodology (RSM) is employed to develop an optimum media for bacteria growth. E. coli DH5 harboring phytase gene from Mycobacterium smegmatis is used as a model to develop the modified growth media. At the end of the study, the effectiveness of the newly developed growth media was also tested on few recombinant proteins.
format	Monograph
author	Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun Nuge, Tamrin
author_facet	Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun Nuge, Tamrin
author_sort	Mohd. Salleh, Hamzah
title	Development of new media formulation for the improvement of recombinant protein expression in E.Coli
title_short	Development of new media formulation for the improvement of recombinant protein expression in E.Coli
title_full	Development of new media formulation for the improvement of recombinant protein expression in E.Coli
title_fullStr	Development of new media formulation for the improvement of recombinant protein expression in E.Coli
title_full_unstemmed	Development of new media formulation for the improvement of recombinant protein expression in E.Coli
title_sort	development of new media formulation for the improvement of recombinant protein expression in e.coli
publisher	s.n
publishDate	2012
url	http://irep.iium.edu.my/33684/ http://irep.iium.edu.my/33684/ http://irep.iium.edu.my/33684/1/EDW_A11-159-950.pdf
first_indexed	2023-09-18T20:48:42Z
last_indexed	2023-09-18T20:48:42Z
_version_	1777409849121832960

Development of new media formulation for the improvement of recombinant protein expression in E.Coli

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