Transcriptional regulation of aldehyde dehydrogenase 1A1 gene by alternative spliced forms of nuclear factor Y in tumorigenic population of endometrial adenocarcinoma

High activity of aldehyde dehydrogenase (ALDH) is characteristic of normal and cancerous stem cells. Recently, high ALDH expression was shown to be associated with poor prognosis in uterine endometrial adenocarcinoma. The population with high ALDH activity (ALDH-hi) was more invasive, anti-apoptotic...

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Main Authors:	Mamat, Suhana, Ikeda, Jun-Ichiro, Tian, Tian, Wang, Yi, Luo, Wenjuan, Aozasa, Katsuyuki, Morii, Eiichi
Format:	Article
Language:	English
Published:	Sage Publications 2011
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/34093/ http://irep.iium.edu.my/34093/ http://irep.iium.edu.my/34093/ http://irep.iium.edu.my/34093/1/1.pdf

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spelling	iium-340932014-01-10T09:09:20Z http://irep.iium.edu.my/34093/ Transcriptional regulation of aldehyde dehydrogenase 1A1 gene by alternative spliced forms of nuclear factor Y in tumorigenic population of endometrial adenocarcinoma Mamat, Suhana Ikeda, Jun-Ichiro Tian, Tian Wang, Yi Luo, Wenjuan Aozasa, Katsuyuki Morii, Eiichi R Medicine (General) High activity of aldehyde dehydrogenase (ALDH) is characteristic of normal and cancerous stem cells. Recently, high ALDH expression was shown to be associated with poor prognosis in uterine endometrial adenocarcinoma. The population with high ALDH activity (ALDH-hi) was more invasive, anti-apoptotic, and tumorigenic than that with low activity (ALDH-lo). Here, the transcriptional regulation of ALDH1A1 gene, which is responsible for ALDH activity, was examined in endometrial adenocarcinoma. The promoter region of ALDH1A1 contained CCAAT and octamer binding motifs, and their mutation diminished promoter activity. Among CCAAT-recognizing transcription factors, nuclear factor YA (NFYA) was involved in ALDH1A1 transcription. Two alternatively spliced isoforms of NFYA (NFYA-long and NFYA-short) have been reported. The sorted ALDH-hi population of endometrial adenocarcinoma preferentially expressed NFYA-short, whereas ALDH-lo dominantly expressed NFYA-long. NFYA-short possessed higher transactivation ability than did NFYA-long. In addition, an additive effect of NFYA with Oct-1, which recognizes octamer binding motif, was observed in ALDH1A1 transactivation. These results indicate that the alternatively spliced isoforms of NFYA, in cooperation with Oct-1, play an important role in ALDH1A1 expression in endometrial adenocarcinoma. Sage Publications 2011-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/34093/1/1.pdf Mamat, Suhana and Ikeda, Jun-Ichiro and Tian, Tian and Wang, Yi and Luo, Wenjuan and Aozasa, Katsuyuki and Morii, Eiichi (2011) Transcriptional regulation of aldehyde dehydrogenase 1A1 gene by alternative spliced forms of nuclear factor Y in tumorigenic population of endometrial adenocarcinoma. Genes & Cancer, 2 (10). pp. 979-984. ISSN 1947-6027 (O), 1947-6019 (P) http://gan.sagepub.com/content/2/10/979.abstract 10.1177/1947601911436009
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Mamat, Suhana Ikeda, Jun-Ichiro Tian, Tian Wang, Yi Luo, Wenjuan Aozasa, Katsuyuki Morii, Eiichi Transcriptional regulation of aldehyde dehydrogenase 1A1 gene by alternative spliced forms of nuclear factor Y in tumorigenic population of endometrial adenocarcinoma
description	High activity of aldehyde dehydrogenase (ALDH) is characteristic of normal and cancerous stem cells. Recently, high ALDH expression was shown to be associated with poor prognosis in uterine endometrial adenocarcinoma. The population with high ALDH activity (ALDH-hi) was more invasive, anti-apoptotic, and tumorigenic than that with low activity (ALDH-lo). Here, the transcriptional regulation of ALDH1A1 gene, which is responsible for ALDH activity, was examined in endometrial adenocarcinoma. The promoter region of ALDH1A1 contained CCAAT and octamer binding motifs, and their mutation diminished promoter activity. Among CCAAT-recognizing transcription factors, nuclear factor YA (NFYA) was involved in ALDH1A1 transcription. Two alternatively spliced isoforms of NFYA (NFYA-long and NFYA-short) have been reported. The sorted ALDH-hi population of endometrial adenocarcinoma preferentially expressed NFYA-short, whereas ALDH-lo dominantly expressed NFYA-long. NFYA-short possessed higher transactivation ability than did NFYA-long. In addition, an additive effect of NFYA with Oct-1, which recognizes octamer binding motif, was observed in ALDH1A1 transactivation. These results indicate that the alternatively spliced isoforms of NFYA, in cooperation with Oct-1, play an important role in ALDH1A1 expression in endometrial adenocarcinoma.
format	Article
author	Mamat, Suhana Ikeda, Jun-Ichiro Tian, Tian Wang, Yi Luo, Wenjuan Aozasa, Katsuyuki Morii, Eiichi
author_facet	Mamat, Suhana Ikeda, Jun-Ichiro Tian, Tian Wang, Yi Luo, Wenjuan Aozasa, Katsuyuki Morii, Eiichi
author_sort	Mamat, Suhana
title	Transcriptional regulation of aldehyde dehydrogenase 1A1 gene by alternative spliced forms of nuclear factor Y in tumorigenic population of endometrial adenocarcinoma
title_short	Transcriptional regulation of aldehyde dehydrogenase 1A1 gene by alternative spliced forms of nuclear factor Y in tumorigenic population of endometrial adenocarcinoma
title_full	Transcriptional regulation of aldehyde dehydrogenase 1A1 gene by alternative spliced forms of nuclear factor Y in tumorigenic population of endometrial adenocarcinoma
title_fullStr	Transcriptional regulation of aldehyde dehydrogenase 1A1 gene by alternative spliced forms of nuclear factor Y in tumorigenic population of endometrial adenocarcinoma
title_full_unstemmed	Transcriptional regulation of aldehyde dehydrogenase 1A1 gene by alternative spliced forms of nuclear factor Y in tumorigenic population of endometrial adenocarcinoma
title_sort	transcriptional regulation of aldehyde dehydrogenase 1a1 gene by alternative spliced forms of nuclear factor y in tumorigenic population of endometrial adenocarcinoma
publisher	Sage Publications
publishDate	2011
url	http://irep.iium.edu.my/34093/ http://irep.iium.edu.my/34093/ http://irep.iium.edu.my/34093/ http://irep.iium.edu.my/34093/1/1.pdf
first_indexed	2023-09-18T20:49:12Z
last_indexed	2023-09-18T20:49:12Z
_version_	1777409879754932224

Transcriptional regulation of aldehyde dehydrogenase 1A1 gene by alternative spliced forms of nuclear factor Y in tumorigenic population of endometrial adenocarcinoma

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