Green synthesis of gold nanoparticles using candida cylindracea culture supernatant

The green synthesis of nanoparticles that have environmentally acceptable solvent systems and ecofriendly reducing agents is of great importance. Meanwhile, the synthesis of metal nanoparticles of different sizes, shapes, chemical composition and controlled monodispersity is a key area of researc...

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Main Authors: Noorbatcha, Ibrahim Ali, Zulkifli, Saifunnasr, Salleh, Mohd. Hamzah
Format: Conference or Workshop Item
Language:English
English
English
Published: 2013
Subjects:
Online Access:http://irep.iium.edu.my/34114/
http://irep.iium.edu.my/34114/3/ICBIOE_2013_Cover_Page_till_Table_of_contents.pdf
http://irep.iium.edu.my/34114/1/AuNP_-_ICBioE2013_proceedings_paper_pp270-273-.pdf
http://irep.iium.edu.my/34114/2/Brochure.pdf
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recordtype eprints
spelling iium-341142014-01-15T01:17:47Z http://irep.iium.edu.my/34114/ Green synthesis of gold nanoparticles using candida cylindracea culture supernatant Noorbatcha, Ibrahim Ali Zulkifli, Saifunnasr Salleh, Mohd. Hamzah TP248.13 Biotechnology The green synthesis of nanoparticles that have environmentally acceptable solvent systems and ecofriendly reducing agents is of great importance. Meanwhile, the synthesis of metal nanoparticles of different sizes, shapes, chemical composition and controlled monodispersity is a key area of research in nanotechnology because of their fascinating physical properties and technological applications. As such, biosynthesis of gold nanoparticles with small size and biostability is vital and used in various biomedical applications. In this paper we report the extracellular biosynthesis of gold nanoparticles (AuNPs) by using Candida cylindracea (also known as Candida rugosa), yeast. In the biosynthesis of AuNPs using this yeast, the cultured yeast was exposed to the chloroauric trihydrate. The bioreduction process involves Au+ ions which are reduced to metallic AuNPs through the catalytic effect of the extracellular enzyme. These gold nanoparticles were characterized by the means of UV–Vis spectroscopy, transmission electron microscopy (TEM), Xray Diffraction spectrum (XRD) and Fourier transform infrared spectroscopy (FTIR). UV–visible spectrum of the aqueous medium containing auric ion showed a peak at 530 nm corresponding to the surface plasmon resonance of gold nanoparticles. The intensity of the colour was found to be increases with respect of time. TEM micrograph showed the formation of well-dispersed gold nanoparticles in the range of 10–30 nm with spherical and triangular shape. Fourier transform infrared spectroscopy revealed possible involvement of reductive groups on the surfaces of nanoparticles. Hence the present study enlightens the green chemistry approach on the production of gold nanoparticles using a microorganism. In comparison to chemical synthesis, the synthesis of gold nanoparticles by microbial source is the most reliable method of production and yield. 2013-07-02 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/34114/3/ICBIOE_2013_Cover_Page_till_Table_of_contents.pdf application/pdf en http://irep.iium.edu.my/34114/1/AuNP_-_ICBioE2013_proceedings_paper_pp270-273-.pdf application/pdf en http://irep.iium.edu.my/34114/2/Brochure.pdf Noorbatcha, Ibrahim Ali and Zulkifli, Saifunnasr and Salleh, Mohd. Hamzah (2013) Green synthesis of gold nanoparticles using candida cylindracea culture supernatant. In: International Conference on Biotechnology Engineering (ICBioE 2013), 2-4 July 2013, Kuala Lumpur, Malaysia.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Noorbatcha, Ibrahim Ali
Zulkifli, Saifunnasr
Salleh, Mohd. Hamzah
Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
description The green synthesis of nanoparticles that have environmentally acceptable solvent systems and ecofriendly reducing agents is of great importance. Meanwhile, the synthesis of metal nanoparticles of different sizes, shapes, chemical composition and controlled monodispersity is a key area of research in nanotechnology because of their fascinating physical properties and technological applications. As such, biosynthesis of gold nanoparticles with small size and biostability is vital and used in various biomedical applications. In this paper we report the extracellular biosynthesis of gold nanoparticles (AuNPs) by using Candida cylindracea (also known as Candida rugosa), yeast. In the biosynthesis of AuNPs using this yeast, the cultured yeast was exposed to the chloroauric trihydrate. The bioreduction process involves Au+ ions which are reduced to metallic AuNPs through the catalytic effect of the extracellular enzyme. These gold nanoparticles were characterized by the means of UV–Vis spectroscopy, transmission electron microscopy (TEM), Xray Diffraction spectrum (XRD) and Fourier transform infrared spectroscopy (FTIR). UV–visible spectrum of the aqueous medium containing auric ion showed a peak at 530 nm corresponding to the surface plasmon resonance of gold nanoparticles. The intensity of the colour was found to be increases with respect of time. TEM micrograph showed the formation of well-dispersed gold nanoparticles in the range of 10–30 nm with spherical and triangular shape. Fourier transform infrared spectroscopy revealed possible involvement of reductive groups on the surfaces of nanoparticles. Hence the present study enlightens the green chemistry approach on the production of gold nanoparticles using a microorganism. In comparison to chemical synthesis, the synthesis of gold nanoparticles by microbial source is the most reliable method of production and yield.
format Conference or Workshop Item
author Noorbatcha, Ibrahim Ali
Zulkifli, Saifunnasr
Salleh, Mohd. Hamzah
author_facet Noorbatcha, Ibrahim Ali
Zulkifli, Saifunnasr
Salleh, Mohd. Hamzah
author_sort Noorbatcha, Ibrahim Ali
title Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
title_short Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
title_full Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
title_fullStr Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
title_full_unstemmed Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
title_sort green synthesis of gold nanoparticles using candida cylindracea culture supernatant
publishDate 2013
url http://irep.iium.edu.my/34114/
http://irep.iium.edu.my/34114/3/ICBIOE_2013_Cover_Page_till_Table_of_contents.pdf
http://irep.iium.edu.my/34114/1/AuNP_-_ICBioE2013_proceedings_paper_pp270-273-.pdf
http://irep.iium.edu.my/34114/2/Brochure.pdf
first_indexed 2023-09-18T20:49:13Z
last_indexed 2023-09-18T20:49:13Z
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