Green synthesis of gold nanoparticles using candida cylindracea culture supernatant

The green synthesis of nanoparticles that have environmentally acceptable solvent systems and ecofriendly reducing agents is of great importance. Meanwhile, the synthesis of metal nanoparticles of different sizes, shapes, chemical composition and controlled monodispersity is a key area of researc...

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Main Authors:	Noorbatcha, Ibrahim Ali, Zulkifli, Saifunnasr, Salleh, Mohd. Hamzah
Format:	Conference or Workshop Item
Language:	English English English
Published:	2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/34114/ http://irep.iium.edu.my/34114/3/ICBIOE_2013_Cover_Page_till_Table_of_contents.pdf http://irep.iium.edu.my/34114/1/AuNP_-_ICBioE2013_proceedings_paper_pp270-273-.pdf http://irep.iium.edu.my/34114/2/Brochure.pdf

id	iium-34114
recordtype	eprints
spelling	iium-341142014-01-15T01:17:47Z http://irep.iium.edu.my/34114/ Green synthesis of gold nanoparticles using candida cylindracea culture supernatant Noorbatcha, Ibrahim Ali Zulkifli, Saifunnasr Salleh, Mohd. Hamzah TP248.13 Biotechnology The green synthesis of nanoparticles that have environmentally acceptable solvent systems and ecofriendly reducing agents is of great importance. Meanwhile, the synthesis of metal nanoparticles of different sizes, shapes, chemical composition and controlled monodispersity is a key area of research in nanotechnology because of their fascinating physical properties and technological applications. As such, biosynthesis of gold nanoparticles with small size and biostability is vital and used in various biomedical applications. In this paper we report the extracellular biosynthesis of gold nanoparticles (AuNPs) by using Candida cylindracea (also known as Candida rugosa), yeast. In the biosynthesis of AuNPs using this yeast, the cultured yeast was exposed to the chloroauric trihydrate. The bioreduction process involves Au+ ions which are reduced to metallic AuNPs through the catalytic effect of the extracellular enzyme. These gold nanoparticles were characterized by the means of UV–Vis spectroscopy, transmission electron microscopy (TEM), Xray Diffraction spectrum (XRD) and Fourier transform infrared spectroscopy (FTIR). UV–visible spectrum of the aqueous medium containing auric ion showed a peak at 530 nm corresponding to the surface plasmon resonance of gold nanoparticles. The intensity of the colour was found to be increases with respect of time. TEM micrograph showed the formation of well-dispersed gold nanoparticles in the range of 10–30 nm with spherical and triangular shape. Fourier transform infrared spectroscopy revealed possible involvement of reductive groups on the surfaces of nanoparticles. Hence the present study enlightens the green chemistry approach on the production of gold nanoparticles using a microorganism. In comparison to chemical synthesis, the synthesis of gold nanoparticles by microbial source is the most reliable method of production and yield. 2013-07-02 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/34114/3/ICBIOE_2013_Cover_Page_till_Table_of_contents.pdf application/pdf en http://irep.iium.edu.my/34114/1/AuNP_-_ICBioE2013_proceedings_paper_pp270-273-.pdf application/pdf en http://irep.iium.edu.my/34114/2/Brochure.pdf Noorbatcha, Ibrahim Ali and Zulkifli, Saifunnasr and Salleh, Mohd. Hamzah (2013) Green synthesis of gold nanoparticles using candida cylindracea culture supernatant. In: International Conference on Biotechnology Engineering (ICBioE 2013), 2-4 July 2013, Kuala Lumpur, Malaysia.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Noorbatcha, Ibrahim Ali Zulkifli, Saifunnasr Salleh, Mohd. Hamzah Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
description	The green synthesis of nanoparticles that have environmentally acceptable solvent systems and ecofriendly reducing agents is of great importance. Meanwhile, the synthesis of metal nanoparticles of different sizes, shapes, chemical composition and controlled monodispersity is a key area of research in nanotechnology because of their fascinating physical properties and technological applications. As such, biosynthesis of gold nanoparticles with small size and biostability is vital and used in various biomedical applications. In this paper we report the extracellular biosynthesis of gold nanoparticles (AuNPs) by using Candida cylindracea (also known as Candida rugosa), yeast. In the biosynthesis of AuNPs using this yeast, the cultured yeast was exposed to the chloroauric trihydrate. The bioreduction process involves Au+ ions which are reduced to metallic AuNPs through the catalytic effect of the extracellular enzyme. These gold nanoparticles were characterized by the means of UV–Vis spectroscopy, transmission electron microscopy (TEM), Xray Diffraction spectrum (XRD) and Fourier transform infrared spectroscopy (FTIR). UV–visible spectrum of the aqueous medium containing auric ion showed a peak at 530 nm corresponding to the surface plasmon resonance of gold nanoparticles. The intensity of the colour was found to be increases with respect of time. TEM micrograph showed the formation of well-dispersed gold nanoparticles in the range of 10–30 nm with spherical and triangular shape. Fourier transform infrared spectroscopy revealed possible involvement of reductive groups on the surfaces of nanoparticles. Hence the present study enlightens the green chemistry approach on the production of gold nanoparticles using a microorganism. In comparison to chemical synthesis, the synthesis of gold nanoparticles by microbial source is the most reliable method of production and yield.
format	Conference or Workshop Item
author	Noorbatcha, Ibrahim Ali Zulkifli, Saifunnasr Salleh, Mohd. Hamzah
author_facet	Noorbatcha, Ibrahim Ali Zulkifli, Saifunnasr Salleh, Mohd. Hamzah
author_sort	Noorbatcha, Ibrahim Ali
title	Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
title_short	Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
title_full	Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
title_fullStr	Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
title_full_unstemmed	Green synthesis of gold nanoparticles using candida cylindracea culture supernatant
title_sort	green synthesis of gold nanoparticles using candida cylindracea culture supernatant
publishDate	2013
url	http://irep.iium.edu.my/34114/ http://irep.iium.edu.my/34114/3/ICBIOE_2013_Cover_Page_till_Table_of_contents.pdf http://irep.iium.edu.my/34114/1/AuNP_-_ICBioE2013_proceedings_paper_pp270-273-.pdf http://irep.iium.edu.my/34114/2/Brochure.pdf
first_indexed	2023-09-18T20:49:13Z
last_indexed	2023-09-18T20:49:13Z
_version_	1777409881651806208

Green synthesis of gold nanoparticles using candida cylindracea culture supernatant

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