Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma

Background: Paraoxonase 1 (PON1) is a high density lipoprotein (HDL) associated enzyme that is known for its function to hydrolyze organophosphate (OPs) into a relatively harmless substance and inhibit oxidative modification of low density lipoprotein (LDL). It is among the commonly studied biochemi...

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Main Authors:	Abdullah, Nor Zamzila, Ahmad Sufi, Nur Farhah, A. Talib, Norlelawati, Ku Zaifah, Norsidah, Muhammad, Naznin, Mohamed, Abdul Hadi
Format:	Article
Language:	English
Published:	College of Pathologists, Academy of Medicine Malaysia 2012
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/34288/ http://irep.iium.edu.my/34288/ http://irep.iium.edu.my/34288/1/abstracts-annual-meeting_46.pdf

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spelling	iium-342882015-04-07T03:17:36Z http://irep.iium.edu.my/34288/ Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma Abdullah, Nor Zamzila Ahmad Sufi, Nur Farhah A. Talib, Norlelawati Ku Zaifah, Norsidah Muhammad, Naznin Mohamed, Abdul Hadi RB Pathology Background: Paraoxonase 1 (PON1) is a high density lipoprotein (HDL) associated enzyme that is known for its function to hydrolyze organophosphate (OPs) into a relatively harmless substance and inhibit oxidative modification of low density lipoprotein (LDL). It is among the commonly studied biochemical markers for cardiovascular diseases. PON 1 activity is usually measured in serum (from plain container) while some other relevant cardiovascular parameters require anti-coagulated plasma. Collection of blood in many types of container may increase the cost and require a larger amount of blood to be collected. Apart from plasma in lithium heparin, the reliability of plasma in other anti-coagulated container such as potassium-citrate, potassium- oxalate/sodium-fluoride and CPDA (from blood collection bag) on PON 1 activity have not yet been clearly ascertained. The aim of this study was to compare and to study the correlation between the PON1 activity in serum and in plasma collected in various anti-coagulated containers. Methods: An experimental study was carried out on 50 volunteers. Blood samples were collected in plain container and containers with potassium-EDTA, lithium-heparin, potassium-citrate, potassium-oxalate/sodium-fluoride and CPDA. Serum and plasma were analyzed for PON1 activity spectrophotometrically after the hydrolysis of substrates paraoxon. Results: The PON1 activity in plasma from lithium-heparin container was slightly reduced but not statistically different (p=0.062) from that of serum (272.95U/L±85.11 vs 288.95±91). However, the PON1 activity was significantly reduced (p<0.001) to 224.13±74.78 U/L, 211.46±64.18 U/L, 184.32±60.06 U/L and 48.00±45.91 U/L respectively in plasma from potassium-citrate, CPDA, potassium-oxalate/sodium-flouride and potassium-EDTA container. There were significant positive correlations (p<0.001) between PON1 activity in serum with PON1 activity in all anti-coagulated plasma except for plasma in potassium-EDTA container (r=0.27, p=0.06). Conclusion: Our finding suggested that only serum and plasma in lithium-heparin container are suitable for the analysis of PON1 activity. College of Pathologists, Academy of Medicine Malaysia 2012-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/34288/1/abstracts-annual-meeting_46.pdf Abdullah, Nor Zamzila and Ahmad Sufi, Nur Farhah and A. Talib, Norlelawati and Ku Zaifah, Norsidah and Muhammad, Naznin and Mohamed, Abdul Hadi (2012) Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma. The Malaysian Journal of Pathology, 34 (2). p. 216. ISSN 0126-8635 http://www.mjpath.org.my/2012.2/index.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology Abdullah, Nor Zamzila Ahmad Sufi, Nur Farhah A. Talib, Norlelawati Ku Zaifah, Norsidah Muhammad, Naznin Mohamed, Abdul Hadi Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
description	Background: Paraoxonase 1 (PON1) is a high density lipoprotein (HDL) associated enzyme that is known for its function to hydrolyze organophosphate (OPs) into a relatively harmless substance and inhibit oxidative modification of low density lipoprotein (LDL). It is among the commonly studied biochemical markers for cardiovascular diseases. PON 1 activity is usually measured in serum (from plain container) while some other relevant cardiovascular parameters require anti-coagulated plasma. Collection of blood in many types of container may increase the cost and require a larger amount of blood to be collected. Apart from plasma in lithium heparin, the reliability of plasma in other anti-coagulated container such as potassium-citrate, potassium- oxalate/sodium-fluoride and CPDA (from blood collection bag) on PON 1 activity have not yet been clearly ascertained. The aim of this study was to compare and to study the correlation between the PON1 activity in serum and in plasma collected in various anti-coagulated containers. Methods: An experimental study was carried out on 50 volunteers. Blood samples were collected in plain container and containers with potassium-EDTA, lithium-heparin, potassium-citrate, potassium-oxalate/sodium-fluoride and CPDA. Serum and plasma were analyzed for PON1 activity spectrophotometrically after the hydrolysis of substrates paraoxon. Results: The PON1 activity in plasma from lithium-heparin container was slightly reduced but not statistically different (p=0.062) from that of serum (272.95U/L±85.11 vs 288.95±91). However, the PON1 activity was significantly reduced (p<0.001) to 224.13±74.78 U/L, 211.46±64.18 U/L, 184.32±60.06 U/L and 48.00±45.91 U/L respectively in plasma from potassium-citrate, CPDA, potassium-oxalate/sodium-flouride and potassium-EDTA container. There were significant positive correlations (p<0.001) between PON1 activity in serum with PON1 activity in all anti-coagulated plasma except for plasma in potassium-EDTA container (r=0.27, p=0.06). Conclusion: Our finding suggested that only serum and plasma in lithium-heparin container are suitable for the analysis of PON1 activity.
format	Article
author	Abdullah, Nor Zamzila Ahmad Sufi, Nur Farhah A. Talib, Norlelawati Ku Zaifah, Norsidah Muhammad, Naznin Mohamed, Abdul Hadi
author_facet	Abdullah, Nor Zamzila Ahmad Sufi, Nur Farhah A. Talib, Norlelawati Ku Zaifah, Norsidah Muhammad, Naznin Mohamed, Abdul Hadi
author_sort	Abdullah, Nor Zamzila
title	Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
title_short	Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
title_full	Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
title_fullStr	Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
title_full_unstemmed	Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
title_sort	paraoxonase-1 (pon1) activity in serum and various anticoagulated-plasma
publisher	College of Pathologists, Academy of Medicine Malaysia
publishDate	2012
url	http://irep.iium.edu.my/34288/ http://irep.iium.edu.my/34288/ http://irep.iium.edu.my/34288/1/abstracts-annual-meeting_46.pdf
first_indexed	2023-09-18T20:49:27Z
last_indexed	2023-09-18T20:49:27Z
_version_	1777409895918731264

Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma

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