Isolation of fungi producing Cellulases from Cocoa Pod (Theobroma Cacao L.)
Cellulases are a complex inducible enzyme that able to degrade cellulose. This study investigates the isolation of fungi producing cellulase from mouldy cocoa pod (Theobroma cacao L.). Screening of cellulolytic isolates by using a congo red test were carried out on carboxymethyl cellulose (CMC) agar...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/34859/ http://irep.iium.edu.my/34859/1/EXTENDED_ABSTRACT-_1163.pdf http://irep.iium.edu.my/34859/4/ISOLATION_OF_FUNGI_PRODUCING_CELLULASES_FROM_COCOA_POD.pdf |
| Summary: | Cellulases are a complex inducible enzyme that able to degrade cellulose. This study investigates the isolation of fungi producing cellulase from mouldy cocoa pod (Theobroma cacao L.). Screening of cellulolytic isolates by using a congo red test were carried out on carboxymethyl cellulose (CMC) agar plates. The production of cellulases by isolated-cocoa pod fungi in fermentation using cocoa pod husks as substrate was performed. Fungal-cellulases activity of filter paper activity (FPase), carboxylmethyl cellulase (CMCase) and reducing sugar concentration were determined. |
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