In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load
Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV pati...
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| Format: | Conference or Workshop Item |
| Language: | English |
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2013
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| Online Access: | http://irep.iium.edu.my/34879/ http://irep.iium.edu.my/34879/1/IRIIE-2013-Abstact-Category.pdf |
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iium-34879 |
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eprints |
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iium-348792014-01-28T04:48:55Z http://irep.iium.edu.my/34879/ In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin QR355 Virology Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV patients and 53 from patients with negative anti-HCV. Viral RNA was extrcated and subjected for RT-PCR or semi-nested RT-PCR. Results showed high sensitivity of the assay which can detect up to 2.15 IU/ml of viral load. The specificity of the assay was also evaluated. Thus, the extablished protocol was reliable for the detection of HCV-RNA from serum. 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/34879/1/IRIIE-2013-Abstact-Category.pdf Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen and Abdullah, Nasuruddin (2013) In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load. In: IIUM Research, Invention and Innovation Exhibition 2013, 19 - 20 February 2013, Cultural Activity Centre (CAC) and KAED Gallery, IIUM. |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QR355 Virology |
| spellingShingle |
QR355 Virology Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| description |
Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV patients and 53 from patients with negative anti-HCV. Viral RNA was extrcated and subjected for RT-PCR or semi-nested RT-PCR. Results showed high sensitivity of the assay which can detect up to 2.15 IU/ml of viral load. The specificity of the assay was also evaluated. Thus, the extablished protocol was reliable for the detection of HCV-RNA from serum. |
| format |
Conference or Workshop Item |
| author |
Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin |
| author_facet |
Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin |
| author_sort |
Hamzah, Hairul Aini |
| title |
In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| title_short |
In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| title_full |
In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| title_fullStr |
In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| title_full_unstemmed |
In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| title_sort |
in-house semi-nested reverse transcription polymerase chain reaction for the detection of hcv-rna in serum with low viral load |
| publishDate |
2013 |
| url |
http://irep.iium.edu.my/34879/ http://irep.iium.edu.my/34879/1/IRIIE-2013-Abstact-Category.pdf |
| first_indexed |
2023-09-18T20:50:09Z |
| last_indexed |
2023-09-18T20:50:09Z |
| _version_ |
1777409940313341952 |