Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger

A number of media components influencing α-amylase production by Aspergillus niger O103A were studied using local bitter variety of cassava (Manihot escuelenta) as the main substrate. The media components (cassava, maltose, glucose, yeast extract, urea, NH4NO3, KH2PO4, CaCl2, MgSO4.7H2O, Na...

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Main Authors:	I.Khalaf, Abu Bakir, Alam, Md. Zahangir, Hamzah, Mohd. Salleh
Format:	Conference or Workshop Item
Language:	English
Published:	2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/34998/ http://irep.iium.edu.my/34998/1/ICBioE-14-abu_bakir.PDF

id	iium-34998
recordtype	eprints
spelling	iium-349982014-09-15T06:37:49Z http://irep.iium.edu.my/34998/ Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger I.Khalaf, Abu Bakir Alam, Md. Zahangir Hamzah, Mohd. Salleh TP248.13 Biotechnology A number of media components influencing α-amylase production by Aspergillus niger O103A were studied using local bitter variety of cassava (Manihot escuelenta) as the main substrate. The media components (cassava, maltose, glucose, yeast extract, urea, NH4NO3, KH2PO4, CaCl2, MgSO4.7H2O, NaCl, and FeSO4.7H2O) were analyzed using Plackett– Burman design. Optimal concentrations of the components contributing positively to enzyme production were further determined using one factor at a time (OFAT) method. Among different nitrogen and supplementary carbon sources tested, yeast extract 2 (g/l) and maltose 3 (g/L) were most effective for enzyme production. Where the optimized media resulted in higher enzyme titre of 42 U/ml. The results indicated the efficiency of using PB design and (OFAT) methodology for screening and optimization processes. 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/34998/1/ICBioE-14-abu_bakir.PDF I.Khalaf, Abu Bakir and Alam, Md. Zahangir and Hamzah, Mohd. Salleh (2013) Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger. In: 3rd International Conference on Biotechnology Engineering (ICBIoE 2013), 2-4 July 2013, Kuala Lumpur, Malaysia.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology I.Khalaf, Abu Bakir Alam, Md. Zahangir Hamzah, Mohd. Salleh Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger
description	A number of media components influencing α-amylase production by Aspergillus niger O103A were studied using local bitter variety of cassava (Manihot escuelenta) as the main substrate. The media components (cassava, maltose, glucose, yeast extract, urea, NH4NO3, KH2PO4, CaCl2, MgSO4.7H2O, NaCl, and FeSO4.7H2O) were analyzed using Plackett– Burman design. Optimal concentrations of the components contributing positively to enzyme production were further determined using one factor at a time (OFAT) method. Among different nitrogen and supplementary carbon sources tested, yeast extract 2 (g/l) and maltose 3 (g/L) were most effective for enzyme production. Where the optimized media resulted in higher enzyme titre of 42 U/ml. The results indicated the efficiency of using PB design and (OFAT) methodology for screening and optimization processes.
format	Conference or Workshop Item
author	I.Khalaf, Abu Bakir Alam, Md. Zahangir Hamzah, Mohd. Salleh
author_facet	I.Khalaf, Abu Bakir Alam, Md. Zahangir Hamzah, Mohd. Salleh
author_sort	I.Khalaf, Abu Bakir
title	Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger
title_short	Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger
title_full	Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger
title_fullStr	Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger
title_full_unstemmed	Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger
title_sort	optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger
publishDate	2013
url	http://irep.iium.edu.my/34998/ http://irep.iium.edu.my/34998/1/ICBioE-14-abu_bakir.PDF
first_indexed	2023-09-18T20:50:18Z
last_indexed	2023-09-18T20:50:18Z
_version_	1777409949427564544

Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger

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