Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion
Several factors affecting lipase production by Candida cylindracea were studied using palm kernel cake (PKC) as the basal medium. The factors included for media components: peptone, yeast extract, glucose, NaNO3, MgSO4, olive oil and Tween-80 and process conditions: inoculum, temperature, pH...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/34999/ http://irep.iium.edu.my/34999/1/ICBioE-14-amal.PDF |
| Summary: | Several factors affecting lipase production by Candida cylindracea were studied using palm kernel
cake (PKC) as the basal medium. The factors included for media components: peptone, yeast
extract, glucose, NaNO3, MgSO4, olive oil and Tween-80 and process conditions: inoculum,
temperature, pH and moisture content. Using Plackett–Burman (PB) design for screening, the
highest lipase activity obtained was 9.41 U/ml in the absence of glucose, peptone and MgSO4. The
factors that contributed the most in lipase production were further investigated using one-factor-at-
a-time (OFAT) experiments in order to find the optimal conditions for the lipase productivity. An
activity of 30.0 U/ml was obtained after 144h of fermentation by the end of this study which was
used for optimization afterword. |
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