Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion

Several factors affecting lipase production by Candida cylindracea were studied using palm kernel cake (PKC) as the basal medium. The factors included for media components: peptone, yeast extract, glucose, NaNO3, MgSO4, olive oil and Tween-80 and process conditions: inoculum, temperature, pH...

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Main Authors:	Elgharbawy, Amal, Alam, Md. Zahangir, Hamzah, Mohd. Salleh
Format:	Conference or Workshop Item
Language:	English
Published:	2013
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/34999/ http://irep.iium.edu.my/34999/1/ICBioE-14-amal.PDF

id	iium-34999
recordtype	eprints
spelling	iium-349992014-01-28T03:00:28Z http://irep.iium.edu.my/34999/ Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion Elgharbawy, Amal Alam, Md. Zahangir Hamzah, Mohd. Salleh TP248.13 Biotechnology Several factors affecting lipase production by Candida cylindracea were studied using palm kernel cake (PKC) as the basal medium. The factors included for media components: peptone, yeast extract, glucose, NaNO3, MgSO4, olive oil and Tween-80 and process conditions: inoculum, temperature, pH and moisture content. Using Plackett–Burman (PB) design for screening, the highest lipase activity obtained was 9.41 U/ml in the absence of glucose, peptone and MgSO4. The factors that contributed the most in lipase production were further investigated using one-factor-at- a-time (OFAT) experiments in order to find the optimal conditions for the lipase productivity. An activity of 30.0 U/ml was obtained after 144h of fermentation by the end of this study which was used for optimization afterword. 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/34999/1/ICBioE-14-amal.PDF Elgharbawy, Amal and Alam, Md. Zahangir and Hamzah, Mohd. Salleh (2013) Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion. In: International Conference on Biotechnology Engineering (ICBioE 2013), 2 – 4 July 2013, Kuala Lumpur, Malaysia.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Elgharbawy, Amal Alam, Md. Zahangir Hamzah, Mohd. Salleh Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion
description	Several factors affecting lipase production by Candida cylindracea were studied using palm kernel cake (PKC) as the basal medium. The factors included for media components: peptone, yeast extract, glucose, NaNO3, MgSO4, olive oil and Tween-80 and process conditions: inoculum, temperature, pH and moisture content. Using Plackett–Burman (PB) design for screening, the highest lipase activity obtained was 9.41 U/ml in the absence of glucose, peptone and MgSO4. The factors that contributed the most in lipase production were further investigated using one-factor-at- a-time (OFAT) experiments in order to find the optimal conditions for the lipase productivity. An activity of 30.0 U/ml was obtained after 144h of fermentation by the end of this study which was used for optimization afterword.
format	Conference or Workshop Item
author	Elgharbawy, Amal Alam, Md. Zahangir Hamzah, Mohd. Salleh
author_facet	Elgharbawy, Amal Alam, Md. Zahangir Hamzah, Mohd. Salleh
author_sort	Elgharbawy, Amal
title	Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion
title_short	Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion
title_full	Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion
title_fullStr	Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion
title_full_unstemmed	Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion
title_sort	statistical screening and optimization of factors influence lipase production from palm kernel cake using candida cylindracea by solid-state bioconversion
publishDate	2013
url	http://irep.iium.edu.my/34999/ http://irep.iium.edu.my/34999/1/ICBioE-14-amal.PDF
first_indexed	2023-09-18T20:50:18Z
last_indexed	2023-09-18T20:50:18Z
_version_	1777409949575413760

Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion

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