Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi

Persicaria odorata is a common plant and well known locally as “daun kesum” that is usually used in culinary and have various medicinal properties. This study is conducted to investigate the antimicrobial activity and the chemical constituent of the most active extract. The leaves were extracted usi...

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Main Authors:	Pauzi, Muhd Ridzuan, Hamzah, Hairul Aini, Shah, Anis, Mohd Hassan, Norazian, ., Roesnita, K. S., Aminah
Format:	Article
Language:	English
Published:	Bentham Open 2013
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/35015/ http://irep.iium.edu.my/35015/ http://irep.iium.edu.my/35015/ http://irep.iium.edu.my/35015/5/Ridzuan_paper.pdf

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recordtype	eprints
spelling	iium-350152014-07-23T07:37:02Z http://irep.iium.edu.my/35015/ Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi Pauzi, Muhd Ridzuan Hamzah, Hairul Aini Shah, Anis Mohd Hassan, Norazian ., Roesnita K. S., Aminah QR Microbiology Persicaria odorata is a common plant and well known locally as “daun kesum” that is usually used in culinary and have various medicinal properties. This study is conducted to investigate the antimicrobial activity and the chemical constituent of the most active extract. The leaves were extracted using n-hexane, dichloromethane, methanol and water to produce the respective extracts. All extracts were tested against eight bacterial strains which include gram positive and gram negative bacteria and one fungal strain by using disc diffusion method. In this research erythromycin 15 µg, vancomycin 30 µg and gentamicin 10 µg were used as antibacterial standard whereas miconazole 50 µg were used as antifungal. The antimicrobial activity of the active extract is evaluated quantitatively by using broth microdilution assay. Gas Chromatography-Mass (GCMS) Spectroscopy analysis was used to analyze the chemical constituent of the most active extract. n-Hexane extract was found to be the most active extract which was active against S. aureus (29.3±0.57), S. epidermidis (32.6±1.52), S. pneumonia (11.3±1.52) and S. pyogenes (15.6±1.15). However, the all extracts are inactive against fungi. The extract produced minimum inhibitory concentration (MIC) of 100µg/ml against S. aureus and 50µg/ml each against S. epidermidis, S. pneumonia and S. pyogenes. GCMS analysis has identified Decanal, Caryophyllene, Dodecanal the major constituents of n-hexane extract. The results obtained in this study showed that P. odorata leaves have high potential to be used as natural antibacterial agent against bacterial infections. Bentham Open 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/35015/5/Ridzuan_paper.pdf Pauzi, Muhd Ridzuan and Hamzah, Hairul Aini and Shah, Anis and Mohd Hassan, Norazian and ., Roesnita and K. S., Aminah (2013) Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi. The Open Conference Proceedings Journal, 4 (supp-2). pp. 71-74. ISSN 2210-2892 http://www.benthamscience.com/open/openaccess.php?toprocj/articles/V004/SS0201TOPROCJ/71TOPROCJ.htm 10.2174/2210289201304020071
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Pauzi, Muhd Ridzuan Hamzah, Hairul Aini Shah, Anis Mohd Hassan, Norazian ., Roesnita K. S., Aminah Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi
description	Persicaria odorata is a common plant and well known locally as “daun kesum” that is usually used in culinary and have various medicinal properties. This study is conducted to investigate the antimicrobial activity and the chemical constituent of the most active extract. The leaves were extracted using n-hexane, dichloromethane, methanol and water to produce the respective extracts. All extracts were tested against eight bacterial strains which include gram positive and gram negative bacteria and one fungal strain by using disc diffusion method. In this research erythromycin 15 µg, vancomycin 30 µg and gentamicin 10 µg were used as antibacterial standard whereas miconazole 50 µg were used as antifungal. The antimicrobial activity of the active extract is evaluated quantitatively by using broth microdilution assay. Gas Chromatography-Mass (GCMS) Spectroscopy analysis was used to analyze the chemical constituent of the most active extract. n-Hexane extract was found to be the most active extract which was active against S. aureus (29.3±0.57), S. epidermidis (32.6±1.52), S. pneumonia (11.3±1.52) and S. pyogenes (15.6±1.15). However, the all extracts are inactive against fungi. The extract produced minimum inhibitory concentration (MIC) of 100µg/ml against S. aureus and 50µg/ml each against S. epidermidis, S. pneumonia and S. pyogenes. GCMS analysis has identified Decanal, Caryophyllene, Dodecanal the major constituents of n-hexane extract. The results obtained in this study showed that P. odorata leaves have high potential to be used as natural antibacterial agent against bacterial infections.
format	Article
author	Pauzi, Muhd Ridzuan Hamzah, Hairul Aini Shah, Anis Mohd Hassan, Norazian ., Roesnita K. S., Aminah
author_facet	Pauzi, Muhd Ridzuan Hamzah, Hairul Aini Shah, Anis Mohd Hassan, Norazian ., Roesnita K. S., Aminah
author_sort	Pauzi, Muhd Ridzuan
title	Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi
title_short	Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi
title_full	Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi
title_fullStr	Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi
title_full_unstemmed	Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi
title_sort	antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi
publisher	Bentham Open
publishDate	2013
url	http://irep.iium.edu.my/35015/ http://irep.iium.edu.my/35015/ http://irep.iium.edu.my/35015/ http://irep.iium.edu.my/35015/5/Ridzuan_paper.pdf
first_indexed	2023-09-18T20:50:20Z
last_indexed	2023-09-18T20:50:20Z
_version_	1777409951317098496

Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi

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