Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy

Cell-based regenerative therapies, based on in vitro propagation of stem cells, offer tremendous hope to many individuals suffering from degenerative diseases that were previously deemed untreatable. Due to the self-renewal capacity, multilineage potential, and immunosuppressive property, mesenchyma...

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Main Authors:	Haque, Nazmul, Rahman, Mohammad Tariqur, Abu Kasim, Noor Hayaty, Alabsi, Aied Mohammed
Format:	Article
Language:	English
Published:	Hindawi Publishing Corporation 2013
Subjects:	RM270 Serum therapy. Immunotherapy
Online Access:	http://irep.iium.edu.my/35324/ http://irep.iium.edu.my/35324/ http://irep.iium.edu.my/35324/ http://irep.iium.edu.my/35324/1/632972.pdf

id	iium-35324
recordtype	eprints
spelling	iium-353242014-02-05T03:00:35Z http://irep.iium.edu.my/35324/ Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy Haque, Nazmul Rahman, Mohammad Tariqur Abu Kasim, Noor Hayaty Alabsi, Aied Mohammed RM270 Serum therapy. Immunotherapy Cell-based regenerative therapies, based on in vitro propagation of stem cells, offer tremendous hope to many individuals suffering from degenerative diseases that were previously deemed untreatable. Due to the self-renewal capacity, multilineage potential, and immunosuppressive property, mesenchymal stem cells (MSCs) are considered as an attractive source of stem cells for regenerative therapies. However, poor growth kinetics, early senescence, and genetic instability during in vitro expansion and poor engraftment after transplantation are considered to be among the major disadvantages of MSC-based regenerative therapies. A number of complex inter- and intracellular interactive signaling systems control growth, multiplication, and differentiation of MSCs in their niche. Common laboratory conditions for stem cell culture involve ambient O2 concentration (20%) in contrast to their niche where they usually reside in 2–9% O2. Notably, O2 plays an important role in maintaining stem cell fate in terms of proliferation and differentiation, by regulating hypoxia-inducible factor-1 (HIF-1) mediated expression of different genes. This paper aims to describe and compare the role of normoxia (20% O2) and hypoxia (2–9% O2) on the biology of MSCs. Finally it is concluded that a hypoxic environment can greatly improve growth kinetics, genetic stability, and expression of chemokine receptors during in vitro expansion and eventually can increase efficiency of MSC-based regenerative therapies. ,1,2 ,3 ,1,2 and Hindawi Publishing Corporation 2013-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/35324/1/632972.pdf Haque, Nazmul and Rahman, Mohammad Tariqur and Abu Kasim, Noor Hayaty and Alabsi, Aied Mohammed (2013) Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy. The Scientific World Journal , 2013 (632972). pp. 1-12. ISSN 1537-744X http://www.hindawi.com/journals/tswj/2013/632972/ http://dx.doi.org/10.1155/2013/632972
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM270 Serum therapy. Immunotherapy
spellingShingle	RM270 Serum therapy. Immunotherapy Haque, Nazmul Rahman, Mohammad Tariqur Abu Kasim, Noor Hayaty Alabsi, Aied Mohammed Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy
description	Cell-based regenerative therapies, based on in vitro propagation of stem cells, offer tremendous hope to many individuals suffering from degenerative diseases that were previously deemed untreatable. Due to the self-renewal capacity, multilineage potential, and immunosuppressive property, mesenchymal stem cells (MSCs) are considered as an attractive source of stem cells for regenerative therapies. However, poor growth kinetics, early senescence, and genetic instability during in vitro expansion and poor engraftment after transplantation are considered to be among the major disadvantages of MSC-based regenerative therapies. A number of complex inter- and intracellular interactive signaling systems control growth, multiplication, and differentiation of MSCs in their niche. Common laboratory conditions for stem cell culture involve ambient O2 concentration (20%) in contrast to their niche where they usually reside in 2–9% O2. Notably, O2 plays an important role in maintaining stem cell fate in terms of proliferation and differentiation, by regulating hypoxia-inducible factor-1 (HIF-1) mediated expression of different genes. This paper aims to describe and compare the role of normoxia (20% O2) and hypoxia (2–9% O2) on the biology of MSCs. Finally it is concluded that a hypoxic environment can greatly improve growth kinetics, genetic stability, and expression of chemokine receptors during in vitro expansion and eventually can increase efficiency of MSC-based regenerative therapies. ,1,2 ,3 ,1,2 and
format	Article
author	Haque, Nazmul Rahman, Mohammad Tariqur Abu Kasim, Noor Hayaty Alabsi, Aied Mohammed
author_facet	Haque, Nazmul Rahman, Mohammad Tariqur Abu Kasim, Noor Hayaty Alabsi, Aied Mohammed
author_sort	Haque, Nazmul
title	Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy
title_short	Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy
title_full	Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy
title_fullStr	Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy
title_full_unstemmed	Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy
title_sort	hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy
publisher	Hindawi Publishing Corporation
publishDate	2013
url	http://irep.iium.edu.my/35324/ http://irep.iium.edu.my/35324/ http://irep.iium.edu.my/35324/ http://irep.iium.edu.my/35324/1/632972.pdf
first_indexed	2023-09-18T20:50:39Z
last_indexed	2023-09-18T20:50:39Z
_version_	1777409971875479552

Hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy

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