Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth

Metallothionein (MT), a metal and stress-induced protein, provides transport and storage of metal ions. The current study analyzed the influence of human cord blood leukocyte [Zn] and gravida on leukocyte MT-2A mRNA expression. Samples (n= 47) were collected at the labor rooms of a Government hospit...

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Main Authors:	Rahman, Mohammad Tariqur, Youssof, Ayman Lee, Abu Kassim , Noor Lide, Rashid, Siti Aishah, De Ley, Marc
Format:	Conference or Workshop Item
Language:	English
Published:	2013
Subjects:	QH301 Biology
Online Access:	http://irep.iium.edu.my/35414/ http://irep.iium.edu.my/35414/ http://irep.iium.edu.my/35414/1/MSBMB38_Doc_for_APAR2.pdf

id	iium-35414
recordtype	eprints
spelling	iium-354142014-03-06T03:25:59Z http://irep.iium.edu.my/35414/ Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth Rahman, Mohammad Tariqur Youssof, Ayman Lee Abu Kassim , Noor Lide Rashid, Siti Aishah De Ley, Marc QH301 Biology Metallothionein (MT), a metal and stress-induced protein, provides transport and storage of metal ions. The current study analyzed the influence of human cord blood leukocyte [Zn] and gravida on leukocyte MT-2A mRNA expression. Samples (n= 47) were collected at the labor rooms of a Government hospital in Kuantan, Malaysia. [Zn] in plasma, erythrocytes, and leukocytes were measured by AAS (λ=213. 9 nm). MT-2A isoform specific mRNA was quantified by real-time PCR using total leukocyte RNA. Quantification of MT-2A gene transcription was normalized to the housekeeping gene, β-actin. Significant correlations were found between plasma [Zn] and erythrocyte [Zn] (r = .304; p< .05) and between leukocyte [Zn] and plasma [Zn] (r = .295; p< .05). Leukocyte [Zn] exhibited a significant correlation with MT-2A mRNA level (r = .765; p< .001). Higher levels of MT-2A mRNA was found in primiparous mothers (n= 16; M= 1.108, SD= 1.273) compared to multiparous mothers (n= 30; M= 0.581, SD= 0.711). Path analysis showed that leukocyte [Zn] has a greater influence (regression weight .84; p= .000) on MT-2A mRNA expression compared to gravida (regression weight -.18; p= .021). Finally it is concluded that, in addition to circulatory Zn, gravida contributes to the MT-2A mRNA expression. 1, NOOR LIDE 2, 3, MARC DE LEY4, MOHAMMAD TARIQUR 1 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/35414/1/MSBMB38_Doc_for_APAR2.pdf Rahman, Mohammad Tariqur and Youssof, Ayman Lee and Abu Kassim , Noor Lide and Rashid, Siti Aishah and De Ley, Marc (2013) Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth. In: 38th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology, 28-29 August 2013, Putrajaya Marriott Hotel and Spa, Putrajaya, Malaysia. http://www.msbmb.org
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QH301 Biology
spellingShingle	QH301 Biology Rahman, Mohammad Tariqur Youssof, Ayman Lee Abu Kassim , Noor Lide Rashid, Siti Aishah De Ley, Marc Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth
description	Metallothionein (MT), a metal and stress-induced protein, provides transport and storage of metal ions. The current study analyzed the influence of human cord blood leukocyte [Zn] and gravida on leukocyte MT-2A mRNA expression. Samples (n= 47) were collected at the labor rooms of a Government hospital in Kuantan, Malaysia. [Zn] in plasma, erythrocytes, and leukocytes were measured by AAS (λ=213. 9 nm). MT-2A isoform specific mRNA was quantified by real-time PCR using total leukocyte RNA. Quantification of MT-2A gene transcription was normalized to the housekeeping gene, β-actin. Significant correlations were found between plasma [Zn] and erythrocyte [Zn] (r = .304; p< .05) and between leukocyte [Zn] and plasma [Zn] (r = .295; p< .05). Leukocyte [Zn] exhibited a significant correlation with MT-2A mRNA level (r = .765; p< .001). Higher levels of MT-2A mRNA was found in primiparous mothers (n= 16; M= 1.108, SD= 1.273) compared to multiparous mothers (n= 30; M= 0.581, SD= 0.711). Path analysis showed that leukocyte [Zn] has a greater influence (regression weight .84; p= .000) on MT-2A mRNA expression compared to gravida (regression weight -.18; p= .021). Finally it is concluded that, in addition to circulatory Zn, gravida contributes to the MT-2A mRNA expression. 1, NOOR LIDE 2, 3, MARC DE LEY4, MOHAMMAD TARIQUR 1
format	Conference or Workshop Item
author	Rahman, Mohammad Tariqur Youssof, Ayman Lee Abu Kassim , Noor Lide Rashid, Siti Aishah De Ley, Marc
author_facet	Rahman, Mohammad Tariqur Youssof, Ayman Lee Abu Kassim , Noor Lide Rashid, Siti Aishah De Ley, Marc
author_sort	Rahman, Mohammad Tariqur
title	Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth
title_short	Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth
title_full	Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth
title_fullStr	Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth
title_full_unstemmed	Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth
title_sort	human cord blood leukocyte metallothionein-2a mrna expression is associated with primiparaous and multiparous childbirth
publishDate	2013
url	http://irep.iium.edu.my/35414/ http://irep.iium.edu.my/35414/ http://irep.iium.edu.my/35414/1/MSBMB38_Doc_for_APAR2.pdf
first_indexed	2023-09-18T20:50:46Z
last_indexed	2023-09-18T20:50:46Z
_version_	1777409978968047616

Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth

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