Antioxidant activity of papaya extract during wound healing is enhanced by Se2+ addition
The healing efficiency of cutaneous wound increases when Se2+ is added to the unripe papaya phosphate buffered saline (PBS) extract. This study investigated protection against oxidative damage in cutaneous wound by using Se2+ or Zn2+ added unripe papaya PBS extract; Induced cutaneous wound on rat we...
| Main Authors: | , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/35982/ http://irep.iium.edu.my/35982/ http://irep.iium.edu.my/35982/1/Antioxidant_activity_of_papaya_extract.pdf |
| id |
iium-35982 |
|---|---|
| recordtype |
eprints |
| spelling |
iium-359822014-03-12T09:14:03Z http://irep.iium.edu.my/35982/ Antioxidant activity of papaya extract during wound healing is enhanced by Se2+ addition Nafiu, Bidemi Abdulrazaq Rahman, Mohammad Tariqur Q Science (General) The healing efficiency of cutaneous wound increases when Se2+ is added to the unripe papaya phosphate buffered saline (PBS) extract. This study investigated protection against oxidative damage in cutaneous wound by using Se2+ or Zn2+ added unripe papaya PBS extract; Induced cutaneous wound on rat were topically treated twice/day with 20 μL of extract dissolved in deionised water (5 mg/mL) where Se2+ and Zn2+ was separately added at 0.1 μg/mg extract and 100 μM concentrations respectively. Control groups were treated with deionised water (negative) and solcoseryl (positive). The excised wound homogenate at day 4 and 10 post wounding was used to analyse antioxidants enzyme activities (catalase, superoxide dismutase, glutathione peroxidase) and lipid peroxidation. Se2+ added extract significantly (p< 0.05) reduced the wound size as compared to negative and positive controls throughout the observation period and on days 1 and 7 respectively. Positive control and the extract significantly (p< 0.05) reduced wound size on days 1-5 and 7-9 respectively. Se2+ added extract significantly (p< 0.05) increased the enzymes’ activities on day 4 and 10 compared to negative control and day 4 only when compared to positive control. Remaining treatments significantly (p< 0.05) increased activity of catalase on day 4 and 10 and superoxide dismutase on day 4 only when compared to negative control. All treatments significantly (p< 0.05) decreased lipid peroxidation at day 4 and 10 post wounding. Thus, Se2+ added papaya extracts provide increased protection against oxidative damage during wound repair. Keywords: Papaya extract, wound healing, Se, Zn and antioxidant enzymes 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/35982/1/Antioxidant_activity_of_papaya_extract.pdf Nafiu, Bidemi Abdulrazaq and Rahman, Mohammad Tariqur (2014) Antioxidant activity of papaya extract during wound healing is enhanced by Se2+ addition. In: 1st International Conference on Food Properties (iCFP2014), 24-26 January 2014, Kuala Lumpur, Malaysia. http://www.ieomrs.com/icfp/cfp.html |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
Q Science (General) |
| spellingShingle |
Q Science (General) Nafiu, Bidemi Abdulrazaq Rahman, Mohammad Tariqur Antioxidant activity of papaya extract during wound healing is enhanced by Se2+ addition |
| description |
The healing efficiency of cutaneous wound increases when Se2+ is added to the unripe papaya phosphate buffered saline (PBS) extract. This study investigated protection against oxidative damage in cutaneous wound by using Se2+ or Zn2+ added unripe papaya PBS extract; Induced cutaneous wound on rat were topically treated twice/day with 20 μL of extract dissolved in deionised water (5 mg/mL) where Se2+ and Zn2+ was separately added at 0.1 μg/mg extract and 100 μM concentrations respectively. Control groups were treated with deionised water (negative) and solcoseryl (positive). The excised wound homogenate at day 4 and 10 post wounding was used to analyse antioxidants enzyme activities (catalase, superoxide dismutase, glutathione peroxidase) and lipid peroxidation. Se2+ added extract significantly (p< 0.05) reduced the wound size as compared to negative and positive controls throughout the observation period and on days 1 and 7 respectively. Positive control and the extract significantly (p< 0.05) reduced wound size on days 1-5 and 7-9 respectively. Se2+ added extract significantly (p< 0.05) increased the enzymes’ activities on day 4 and 10 compared to negative control and day 4 only when compared to positive control. Remaining treatments significantly (p< 0.05) increased activity of catalase on day 4 and 10 and superoxide dismutase on day 4 only when compared to negative control. All treatments significantly (p< 0.05) decreased lipid peroxidation at day 4 and 10 post wounding. Thus, Se2+ added papaya extracts provide increased protection against oxidative damage during wound repair.
Keywords: Papaya extract, wound healing, Se, Zn and antioxidant enzymes
|
| format |
Conference or Workshop Item |
| author |
Nafiu, Bidemi Abdulrazaq Rahman, Mohammad Tariqur |
| author_facet |
Nafiu, Bidemi Abdulrazaq Rahman, Mohammad Tariqur |
| author_sort |
Nafiu, Bidemi Abdulrazaq |
| title |
Antioxidant activity of papaya extract during wound healing is enhanced by Se2+ addition |
| title_short |
Antioxidant activity of papaya extract during wound healing is enhanced by Se2+ addition |
| title_full |
Antioxidant activity of papaya extract during wound healing is enhanced by Se2+ addition |
| title_fullStr |
Antioxidant activity of papaya extract during wound healing is enhanced by Se2+ addition |
| title_full_unstemmed |
Antioxidant activity of papaya extract during wound healing is enhanced by Se2+ addition |
| title_sort |
antioxidant activity of papaya extract during wound healing is enhanced by se2+ addition |
| publishDate |
2014 |
| url |
http://irep.iium.edu.my/35982/ http://irep.iium.edu.my/35982/ http://irep.iium.edu.my/35982/1/Antioxidant_activity_of_papaya_extract.pdf |
| first_indexed |
2023-09-18T20:51:30Z |
| last_indexed |
2023-09-18T20:51:30Z |
| _version_ |
1777410025274212352 |