Toxicity and antiplasmodial evaluation of Malaysian Plectranthus amboinicus leaves extract

This study was conducted to evaluate the toxicity and antiplasmodial properties of Malaysian Plectranthus amboinicus. Phytochemical analysis on the crude ethanolic extract of leaves of Malaysian P. amboinicus (Lour) Spreng was carried out to delineate the presence of its chemical constituents like A...

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Main Authors:	Ramli, Norazsida, Oothuman, Pakeer, Bakhtiar, M. Taher, Elhady, Hassan Mohamed
Format:	Conference or Workshop Item
Language:	English
Published:	2013
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/36161/ http://irep.iium.edu.my/36161/ http://irep.iium.edu.my/36161/1/Binder1.pdf

id	iium-36161
recordtype	eprints
spelling	iium-361612017-07-06T01:32:06Z http://irep.iium.edu.my/36161/ Toxicity and antiplasmodial evaluation of Malaysian Plectranthus amboinicus leaves extract Ramli, Norazsida Oothuman, Pakeer Bakhtiar, M. Taher Elhady, Hassan Mohamed RS Pharmacy and materia medica This study was conducted to evaluate the toxicity and antiplasmodial properties of Malaysian Plectranthus amboinicus. Phytochemical analysis on the crude ethanolic extract of leaves of Malaysian P. amboinicus (Lour) Spreng was carried out to delineate the presence of its chemical constituents like Alkaloid, Flavonoid, Saponin, Triterpenoid, Steroid, and Phenolic components. Acute oral toxicity at one dose level of 5000 mg/kg as per OECD Guideline for Testing of Chemicals 423 was conducted to evaluate the safeness of this extract to be consumed by humans. 20 mice were divided into one control group and one experimental group. All the mice were observed for signs of toxicity, mortality, weight changes and histopathological changes. Antimalarial activity of different extract doses of 50, 200, 400 and 1000 mg/kg were tested in vivo against Plasmodium berghei (PZZ1/100) infections in mice (5 mice for each group) during early, established and residual infections. The results revealed that the extract contained only flavonoid. The acute oral toxicity test revealed that no mortality or evidence of adverse effects was seen in the treated mice. Studies on histopathological examination of kidneys and livers showed normal architecture suggesting no morphological changes. The extract significantly reduced the parasitemia by the 50 (p=0.000), 200 (p=0.000) and 400 mg/kg doses (p=0.000) in the in vivo prophylactic assay. The %chemo-suppression was calculated as 83.33% for 50mg/kg dose, 75.62% for 200 mg/kg dose and 90.74% for 400 mg/kg dose. Body weight of all treatment groups; T1, T2, T3 and T4 also showed enhancement after 7 days post-treatment. Statistically no reduction of parasitemia calculated for curative and suppressive test. Thus, the isolation of active compound of this extract may give a promising drug molecule to be used as prophylactic agent of Plasmodium berghei infection in mice 2013-04 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/36161/1/Binder1.pdf Ramli, Norazsida and Oothuman, Pakeer and Bakhtiar, M. Taher and Elhady, Hassan Mohamed (2013) Toxicity and antiplasmodial evaluation of Malaysian Plectranthus amboinicus leaves extract. In: International Malaria Symposium 2013, 16-17 April 2013, Novotel, Kota Kinabalu. https://www.ums.edu.my/v5/index.php/en/archive/162-ims2013-161212.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Ramli, Norazsida Oothuman, Pakeer Bakhtiar, M. Taher Elhady, Hassan Mohamed Toxicity and antiplasmodial evaluation of Malaysian Plectranthus amboinicus leaves extract
description	This study was conducted to evaluate the toxicity and antiplasmodial properties of Malaysian Plectranthus amboinicus. Phytochemical analysis on the crude ethanolic extract of leaves of Malaysian P. amboinicus (Lour) Spreng was carried out to delineate the presence of its chemical constituents like Alkaloid, Flavonoid, Saponin, Triterpenoid, Steroid, and Phenolic components. Acute oral toxicity at one dose level of 5000 mg/kg as per OECD Guideline for Testing of Chemicals 423 was conducted to evaluate the safeness of this extract to be consumed by humans. 20 mice were divided into one control group and one experimental group. All the mice were observed for signs of toxicity, mortality, weight changes and histopathological changes. Antimalarial activity of different extract doses of 50, 200, 400 and 1000 mg/kg were tested in vivo against Plasmodium berghei (PZZ1/100) infections in mice (5 mice for each group) during early, established and residual infections. The results revealed that the extract contained only flavonoid. The acute oral toxicity test revealed that no mortality or evidence of adverse effects was seen in the treated mice. Studies on histopathological examination of kidneys and livers showed normal architecture suggesting no morphological changes. The extract significantly reduced the parasitemia by the 50 (p=0.000), 200 (p=0.000) and 400 mg/kg doses (p=0.000) in the in vivo prophylactic assay. The %chemo-suppression was calculated as 83.33% for 50mg/kg dose, 75.62% for 200 mg/kg dose and 90.74% for 400 mg/kg dose. Body weight of all treatment groups; T1, T2, T3 and T4 also showed enhancement after 7 days post-treatment. Statistically no reduction of parasitemia calculated for curative and suppressive test. Thus, the isolation of active compound of this extract may give a promising drug molecule to be used as prophylactic agent of Plasmodium berghei infection in mice
format	Conference or Workshop Item
author	Ramli, Norazsida Oothuman, Pakeer Bakhtiar, M. Taher Elhady, Hassan Mohamed
author_facet	Ramli, Norazsida Oothuman, Pakeer Bakhtiar, M. Taher Elhady, Hassan Mohamed
author_sort	Ramli, Norazsida
title	Toxicity and antiplasmodial evaluation of Malaysian Plectranthus amboinicus leaves extract
title_short	Toxicity and antiplasmodial evaluation of Malaysian Plectranthus amboinicus leaves extract
title_full	Toxicity and antiplasmodial evaluation of Malaysian Plectranthus amboinicus leaves extract
title_fullStr	Toxicity and antiplasmodial evaluation of Malaysian Plectranthus amboinicus leaves extract
title_full_unstemmed	Toxicity and antiplasmodial evaluation of Malaysian Plectranthus amboinicus leaves extract
title_sort	toxicity and antiplasmodial evaluation of malaysian plectranthus amboinicus leaves extract
publishDate	2013
url	http://irep.iium.edu.my/36161/ http://irep.iium.edu.my/36161/ http://irep.iium.edu.my/36161/1/Binder1.pdf
first_indexed	2023-09-18T20:51:46Z
last_indexed	2023-09-18T20:51:46Z
_version_	1777410041965445120

Toxicity and antiplasmodial evaluation of Malaysian Plectranthus amboinicus leaves extract

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