Heterologous expression of Bromelain in Escherichia coli

In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple. This makes the production of bromelain very diffic...

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Main Authors:	M., Bala, Mohd. Salleh, Hamzah, Amid, Azura, Mel, Maizirwan, Jami, Mohammed Saedi
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/3635/ http://irep.iium.edu.my/3635/ http://irep.iium.edu.my/3635/1/Paper_Bromelain_Bala.pdf

id	iium-3635
recordtype	eprints
spelling	iium-36352011-11-16T02:18:44Z http://irep.iium.edu.my/3635/ Heterologous expression of Bromelain in Escherichia coli M., Bala Mohd. Salleh, Hamzah Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi TP248.13 Biotechnology In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple. This makes the production of bromelain very difficult, less reliable, often contaminated and expensive. In this study, a recombinant bromelain from BL21 A clone was expressed as soluble and insoluble active enzyme. Maximum activity was observed at 4-hour post induction with 0.2% L-arabinose and over 60% of the enzyme was found to be expressed in soluble form. The enzyme fractions were purified using Nickel-NTA spin column. Purification fold and % yield of the purified lysate were found to be 35 and 75% respectively. SDS-PAGE results showed that the purified bromelain exhibited a single band with molecular weight of about 45kDa. 2011-05-17 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/3635/1/Paper_Bromelain_Bala.pdf M., Bala and Mohd. Salleh, Hamzah and Amid, Azura and Mel, Maizirwan and Jami, Mohammed Saedi (2011) Heterologous expression of Bromelain in Escherichia coli. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May, 2011, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology M., Bala Mohd. Salleh, Hamzah Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi Heterologous expression of Bromelain in Escherichia coli
description	In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple. This makes the production of bromelain very difficult, less reliable, often contaminated and expensive. In this study, a recombinant bromelain from BL21 A clone was expressed as soluble and insoluble active enzyme. Maximum activity was observed at 4-hour post induction with 0.2% L-arabinose and over 60% of the enzyme was found to be expressed in soluble form. The enzyme fractions were purified using Nickel-NTA spin column. Purification fold and % yield of the purified lysate were found to be 35 and 75% respectively. SDS-PAGE results showed that the purified bromelain exhibited a single band with molecular weight of about 45kDa.
format	Conference or Workshop Item
author	M., Bala Mohd. Salleh, Hamzah Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi
author_facet	M., Bala Mohd. Salleh, Hamzah Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi
author_sort	M., Bala
title	Heterologous expression of Bromelain in Escherichia coli
title_short	Heterologous expression of Bromelain in Escherichia coli
title_full	Heterologous expression of Bromelain in Escherichia coli
title_fullStr	Heterologous expression of Bromelain in Escherichia coli
title_full_unstemmed	Heterologous expression of Bromelain in Escherichia coli
title_sort	heterologous expression of bromelain in escherichia coli
publishDate	2011
url	http://irep.iium.edu.my/3635/ http://irep.iium.edu.my/3635/ http://irep.iium.edu.my/3635/1/Paper_Bromelain_Bala.pdf
first_indexed	2023-09-18T20:11:32Z
last_indexed	2023-09-18T20:11:32Z
_version_	1777407510210150400

Heterologous expression of Bromelain in Escherichia coli

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